Difference between revisions of "Team:China Tongji/Project"

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         <div class="navOne" id="divProject"><a class="navOneA" id="Project" href="https://2015.igem.org/Team:China_Tongji/Project">Project <img src="https://static.igem.org/mediawiki/2015/0/07/China_Tongji_2015_dropdown_arrow.png"></a>
 
         <div class="navOne" id="divProject"><a class="navOneA" id="Project" href="https://2015.igem.org/Team:China_Tongji/Project">Project <img src="https://static.igem.org/mediawiki/2015/0/07/China_Tongji_2015_dropdown_arrow.png"></a>
 
             <div class="navDrop">
 
             <div class="navDrop">
                     <li class="navTwo" id="navOverview"><a href="https://2015.igem.org/Team:China_Tongji/Project">Overview</a></li>
+
                     <li class="navTwo" id="navOverview"><a>Overview</a></li>
                     <li class="navTwo" id="navBackground"><a href="https://2015.igem.org/Team:China_Tongji/Project">Background</a></li>
+
                     <li class="navTwo" id="navBackground"><a>Background</a></li>
                     <li class="navTwo" id="navDesign"><a href="https://2015.igem.org/Team:China_Tongji/Project">Design</a></li>
+
                     <li class="navTwo" id="navDesign"><a>Design</a></li>
                     <li class="navTwo" id="navProtocol"><a href="https://2015.igem.org/Team:China_Tongji/Project">Protocol</a></li>
+
                     <li class="navTwo" id="navProtocol"><a>Protocol</a></li>
                     <li class="navTwo" id="navSummaryResult"><a href="https://2015.igem.org/Team:China_Tongji/Project">Summary and Result</a></li>
+
                     <li class="navTwo" id="navSummaryResult"><a>Summary and Result</a></li>
 
             </div>
 
             </div>
 
         </div>
 
         </div>
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         <div class="navOne" id="divTeam"><a class="navOneA" id="Team" href="https://2015.igem.org/Team:China_Tongji/Team">Team <img src="https://static.igem.org/mediawiki/2015/0/07/China_Tongji_2015_dropdown_arrow.png"></a>
 
         <div class="navOne" id="divTeam"><a class="navOneA" id="Team" href="https://2015.igem.org/Team:China_Tongji/Team">Team <img src="https://static.igem.org/mediawiki/2015/0/07/China_Tongji_2015_dropdown_arrow.png"></a>
 
             <div class="navDrop">
 
             <div class="navDrop">
                 <li class="navTwo" id="navMembers"><a>Members</a></li>
+
                 <li class="navTwo" id="navMembers"><a href="https://2015.igem.org/Team:China_Tongji/Team">Members</a></li>
                 <li class="navTwo" id="navAdvisor"><a>Advisor</a></li>
+
                 <li class="navTwo" id="navAttributions"><a href="https://2015.igem.org/Team:China_Tongji/Team">Attributions</a></li>
                 <li class="navTwo" id="navInstructor"><a>Instructor</a></li>
+
                 <li class="navTwo" id="navAdvisor"><a href="https://2015.igem.org/Team:China_Tongji/Team">Advisor</a></li>
                 <li class="navTwo" id="navSponsor"><a>Sponsor</a></li>
+
                 <li class="navTwo" id="navSponsor"><a href="https://2015.igem.org/Team:China_Tongji/Team">Sponsor</a></li>
                 <li class="navTwo" id="navContact"><a>Contact</a></li>
+
                 <li class="navTwo" id="navContact"><a href="https://2015.igem.org/Team:China_Tongji/Team">Contact</a></li>
 
             </div>
 
             </div>
 
         </div>
 
         </div>
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<!--content start-->
 
<!--content start-->
 
<div class="bigName">
 
<div class="bigName">
<p align="left">Team<img class="contentImg" width="250px" src="https://static.igem.org/mediawiki/2015/a/a3/China-Tongji-Team-police.png" align="right"></p>
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<p align="left">Project</p>
 
</div>
 
</div>
  
 
<div class="myContent">
 
<div class="myContent">
 
<div id="contentList">
 
<div id="contentList">
         <li class="listOne"><p id="listMembers">1. Members</p>
+
         <li class="listOne"><p id="listOverview">Overview</p></li>
            <ul class="listOneDrop" id="listDropMembers">
+
        <li class="listOne"><p id="listBackground">Background</p>
                <li class="listTwo" onClick="window.scrollTo(0,document.getElementById('first1').offsetTop)">1.1 Xinhong Chen</li>
+
        <ul class="listOneDrop" id="listDropBackground">
                <li class="listTwo" onClick="window.scrollTo(0,document.getElementById('first2').offsetTop)">1.2 Yu Feng</li>
+
                 <li class="listTwo" onClick="window.scrollTo(0,document.getElementById('Challenges').offsetTop)">Challenges</li>
                <li class="listTwo" onClick="window.scrollTo(0,document.getElementById('first3').offsetTop)">1.3 Geyang Zhang</li>
+
                 <li class="listTwo" onClick="window.scrollTo(0,document.getElementById('Solution').offsetTop)">Solution</li>
                <li class="listTwo" onClick="window.scrollTo(0,document.getElementById('first4').offsetTop)">1.4 Wenqi Cao</li>
+
                 <li class="listTwo" onClick="window.scrollTo(0,document.getElementById('first5').offsetTop)">1.5 Zikang Xing</li>
+
                 <li class="listTwo" onClick="window.scrollTo(0,document.getElementById('first6').offsetTop)">1.6 Yini Yang</li>
+
                <li class="listTwo" onClick="window.scrollTo(0,document.getElementById('first7').offsetTop)">1.7 Chenyu Pi</li>
+
                <li class="listTwo" onClick="window.scrollTo(0,document.getElementById('first8').offsetTop)">1.8 Shan qi</li>
+
 
             </ul>
 
             </ul>
 
         </li>
 
         </li>
         <li class="listOne"><p id="listAdvisor">2. Advisor</p></li>
+
         <li class="listOne"><p id="listDesign">Design</p></li>
         <li class="listOne"><p id="listInstructor">3. Instructor</p>
+
         <li class="listOne"><p id="listProtocol">Protocol</p></li>
            <ul class="listOneDrop" id="listDropInstructor">
+
         <li class="listOne"><p id="listSummaryResult">Summary and Result</p></li>
                <li class="listTwo" onClick="window.scrollTo(0,document.getElementById('third1').offsetTop)">3.1 Songcheng Zhu</li>
+
                <li class="listTwo" onClick="window.scrollTo(0,document.getElementById('third2').offsetTop)">3.2 Wei Li</li>
+
            </ul>
+
        </li>
+
        <li class="listOne"><p id="listSponsor">4. Sponsor</p>
+
            <ul class="listOneDrop" id="listDropSponsor">
+
                <li class="listTwo" onClick="window.scrollTo(0,document.getElementById('fourth1').offsetTop)">4.1 Genewiz</li>
+
                <li class="listTwo" onClick="window.scrollTo(0,document.getElementById('fourth2').offsetTop)">4.2 Thorlabs</li>
+
            </ul>
+
        </li>
+
         <li class="listOne"><p id="listContact">5. Contact</p></li>
+
 
     </div>
 
     </div>
 
     <div id=mainContent>
 
     <div id=mainContent>
 
     <!-- maincontent start here -->
 
     <!-- maincontent start here -->
         <div class="divider"></div><div class="fivePx"></div>
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         <center>
        <div>
+
         <img class="contentImg" width="350px" src="https://static.igem.org/mediawiki/2015/c/c4/China-Tongji-teamBug.png"/>
         <img class="contentImg" width="550px" src="https://static.igem.org/mediawiki/2015/a/a3/China-Tongji-Team-police.png" align="center"/>
+
         </center>
         </div>
+
 
     
+
         <p class="titleOne" id="Overview">1. Overview</p>   
        <div class="fivePx"></div>
+
         <p class="titleOne" id="Members">1. Members</p>
+
        <div id="membersWarpper"> 
+
            <div class="membersBlock" id="first1">
+
            <img class="memberImg hoverHand" src="https://static.igem.org/mediawiki/2015/7/77/China-Tongji-Team-chenxinhong.jpg" alt="Xinhong Chen"/>
+
                <div class="fivePx"></div>
+
                <div class="wordWrapper">
+
                    <p class="memberName">Xinhong Chen</p>
+
                    <p class="memberWork">[ The leader of China_Tongji ]</p>
+
                    <p class="memberIntro">A sophomore in Tongji University majoring in Life science.</p>   
+
                    <p class="memberIntro">Filled of crazy ideas,  the “idea maker”.</p>
+
                    <p class="memberIntro">Born in February,  the most creative Aquarius.</p>
+
                    <p class="memberIntro">Constantly exploring new fantasy.</p>
+
                    <p class="memberIntro">Not easy to fall in love but single-minded when it happens.</p>
+
                    <p class="memberIntro">Meet with optogenetic this summer, fall in love with C,elegans.</p>
+
                    <p class="memberIntro">“Igemer” becomes my new mark.</p>
+
                    <p class="memberIntro">Tough but memorable.</p>
+
                </div> 
+
            </div>
+
            <div class="membersBlock" id="first2"> 
+
            <img class="memberImg hoverHand" src="https://static.igem.org/mediawiki/2015/a/ae/China-Tongji-Team-fengyu.jpg" alt="Yu Feng"/>
+
                <div class="fivePx"></div>
+
                <div class="wordWrapper">
+
                    <p class="memberName">Yu Feng</p>
+
                    <p class="memberWork">[ The organizer of China_Tongji, also be in charge of team connection ]</p>
+
                    <p class="memberIntro">A sophomore student who is major in life science.</p>
+
                    <p class="memberIntro">Fall in love with biology when I was a high school student.</p>
+
                    <p class="memberIntro">Always dreaming to be a neurobiologist and study of how the brain works.</p>
+
                    <p class="memberIntro">Be a member of Tongji team last year as an intern student.</p>
+
                    <p class="memberIntro">So proud to be an iGEMer and lead our team to finish the project this time!</p>
+
                    <p class="memberIntro">Good at organizing the team and the whole project.</p>
+
                    <p class="memberIntro">Thoughtfulness and carefulness is my major virtue.</p>
+
                    <p class="memberIntro">9 months hardworking with my lovely friends, I hope we can get what we want in the final competition!</p>   
+
                </div>
+
            </div>
+
            <div class="membersBlock" id="first3">
+
            <img class="memberImg hoverHand" src="https://static.igem.org/mediawiki/2015/0/0c/China-Tongji-Team-zhangeyang.jpg" alt="Geyang Zhang"/>
+
                <div class="fivePx"></div>
+
                <div class="wordWrapper">
+
                    <p class="memberName">Geyang Zhang</p>
+
                    <p class="memberWork">[ C.elegans, the art director ]</p>
+
                    <p class="memberIntro">I’m Geyang Zhang, a girl with song and sunshine;</p>
+
                    <p class="memberIntro">China is my homeland, I value all the creatures it breeds;</p>
+
                    <p class="memberIntro">Nature pushed me into science, I explore deep in its mind;</p>
+
                    <p class="memberIntro">Grown up as time passed by, Tongji is a new place to play;</p>
+
                    <p class="memberIntro">Then I meet with IGEM, where my ideas would be made;</p>
+
                    <p class="memberIntro">Friends gathered for the same goal, we are pursuing for a medal of gold;</p>
+
                    <p class="memberIntro">Worms became our road to go, and light in cells are things we want to know;</p>
+
                    <p class="memberIntro">Some changes may be made by my hands, which is the most exciting thought filled my head.</p>
+
                </div>     
+
            </div>
+
            <div class="membersBlock" id="first4">
+
            <img class="memberImg hoverHand" src="https://static.igem.org/mediawiki/2015/b/b8/China-Tongji-Team-caowenqi.jpg" alt="Wenqi Cao"/>
+
                <div class="fivePx"></div>
+
                <div class="wordWrapper">
+
                    <p class="memberName">Wenqi Cao</p>
+
                    <p class="memberWork">[ The expert in C.elegans’ microinjection ]</p>
+
                    <p class="memberIntro">I am Wenqi cao. A girl.</p>
+
                    <p class="memberIntro">Like reading, travelling, gardening but not microinjecting.</p>
+
                    <p class="memberIntro">Like all lovely creatures including C.elegans.</p>
+
                    <p class="memberIntro">Like the sky when sakura blossoming.</p>
+
                    <p class="memberIntro">Like the wind when stars sparkling.</p>
+
                    <p class="memberIntro">Especially like the microscope when worm fluorescing.</p>
+
                    <p class="memberIntro">Dream to be a writer and travel to all the fascinating places in the world. And it sounds good to be a worm eating E.coli all the time till die.</p>
+
                    <p class="memberIntro">Happy to be a member of Tongji iGem team.</p>
+
                    <p class="memberIntro">Happy to work with my dear friends.</p>
+
                    <p class="memberIntro">Happy to meet you, good luck to whoever you are.</p>
+
                </div>         
+
            </div>
+
            <div class="membersBlock" id="first5"> 
+
            <img class="memberImg hoverHand" src="https://static.igem.org/mediawiki/2015/9/9b/China-Tongji-Team-xingzikang.jpg" alt="Zikang Xing"/>
+
                <div class="fivePx"></div>
+
                <div class="wordWrapper">
+
                    <p class="memberName">Zikang Xing</p>
+
                    <p class="memberWork">[ Be in charge of Molecular Architectures ]</p>
+
                    <br>
+
                    <p class="memberIntro">I am a Chinese, Masculinity is my gender.</p>
+
                    <p class="memberIntro">My major is called bioscience, Science is my mentor. </p> 
+
                    <br>           
+
                    <p class="memberIntro">I don't fear difficulty, Sometimes I like challenge.</p>
+
                    <p class="memberIntro">I only want to keep happy, Optimism never change.</p>
+
                    <br>                 
+
                    <p class="memberIntro">This year I join iGEM, Working hard with classments.</p>
+
                    <p class="memberIntro">We set up a new team, Designing optogenetic C.elegans.</p>
+
                    <br>                 
+
                    <p class="memberIntro">Medal is in our dreams, We will beat other teams!</p>
+
                </div>       
+
            </div>
+
            <div class="membersBlock" id="first6">
+
            <img class="memberImg hoverHand" src="https://static.igem.org/mediawiki/2015/f/fa/China-Tongji-Team-yangyini.jpg" alt="Yini Yang"/>
+
                <div class="fivePx"></div>
+
                <div class="wordWrapper">
+
                    <p class="memberName">Yini Yang</p>
+
                    <p class="memberWork">[ Be in charge of Molecular Architectures ]</p>
+
                    <br>
+
                    <p class="memberIntro">I am a Chinese, Femininity is my gender.</p>
+
                    <p class="memberIntro">My major is called bioscience, Science is my mentor.</p>
+
                    <br>
+
                    <p class="memberIntro">My favorite is nature, I respect each individual.</p>
+
                    <p class="memberIntro">Life is the rarest treasure, All units are equal.</p>
+
                    <br>
+
                    <p class="memberIntro">I have special thought, My creativity never leave.</p>
+
                    <p class="memberIntro">Idea comes from my heart, And I'll work hard to achieve!</p>
+
                    <br>
+
                    <p class="memberIntro">People from other countries, I'd like to make friends with!</p>
+
                </div>       
+
            </div>
+
            <div class="membersBlock" id="first7">   
+
            <img class="memberImg hoverHand" src="https://static.igem.org/mediawiki/2015/b/b9/China-Tongji-Team-pichenyu.jpg" alt="Chenyu Pi"/>
+
                <div class="fivePx"></div>
+
                <div class="wordWrapper">
+
                    <p class="memberName">Chenyu Pi</p>
+
                    <p class="memberWork">[ Director of molecular biology, the leader of E.coli part ]</p>
+
                    <br>
+
                    <p class="memberIntro">Hello, my name is Chenyu Pi.</p>
+
                    <p class="memberIntro">I like to smile and I think smile is the best treasure that god give me.</p>
+
                    <p class="memberIntro">Not only I love science, but also I love sports and music.</p>
+
                    <p class="memberIntro">They may become all my life.</p>
+
                    <p class="memberIntro">In our team, I concentrate on the molecular experiment such as PCR, transformation and ligation.</p>
+
                    <p class="memberIntro">I always try hard to achieve a goal although the result does not conform to our expectation.</p>
+
                    <p class="memberIntro">Come on, fighting!</p>
+
                </div>   
+
            </div>
+
            <div class="membersBlock" id="first8"> 
+
            <img class="memberImg hoverHand" src="https://static.igem.org/mediawiki/2015/2/25/China-Tongji-Team-qishan.jpg" alt="Shan qi"/>
+
                <div class="fivePx"></div>
+
                <div class="wordWrapper">
+
                    <p class="memberName">Shan qi</p>
+
                    <p class="memberWork">[ Be in charge of LED light source building ]</p>
+
                    <br>
+
                    <p class="memberIntro">My name is SHAN QI, and I am a student of Tongji University who is major in life science.</p>
+
                    <p class="memberIntro">I am a big boy from the north district of China. I loves traditional Chinese culture and some Chinese style music.</p>
+
                    <p class="memberIntro">As a student who is major in biology, I likes to find out the right thing.</p>
+
                    <p class="memberIntro">I always insist that the best preparation for tomorrow is doing the best today.</p>
+
                    <p class="memberIntro">It is my first year to join in the iGEM. </p>
+
                    <p class="memberIntro">So I am looking forward to do something fancy in this competition.</p>
+
                </div>       
+
            </div>
+
        </div>
+
       
+
<div class="divider"></div>
+
<p class="titleOne" id="Advisor">2. Advisor</p> 
+
        <div class="divTeam" id="advisor">
+
            <p><img class="teamImg hoverHand" id="advisorImg" src="https://static.igem.org/mediawiki/2015/f/fa/China_Tongji-lijunwu-1-300px.png" alt="advisor image" align="left"/></p>
+
            <p><b>Codename:</b> Master Li</p>
+
            <p><b>Self-introduction:</b> Hi,I am Junwu Li. This is my first time to contact iGEM, I think it is a very meaningful competition. I am very interested in programming, I think it is amazing to realize my ideas by computer language. I feel honored to have chance to design Wiki page for Tongji University Team of China in 2015, meanwhile, I can learn a lot relevant knowledge in the process of designing web page.Anyway, I will try my best to design the wiki page, ensure that the content is easy to read and the interface is beautiful.</p>
+
      </div>
+
       
+
        <div class="divider"></div>
+
<p class="titleOne" id="Instructor">3. Instructor</p> 
+
        <div class="divider"></div>
+
        <p class="titleTwo" id="third1">3.1 Songcheng Zhu</p>           
+
        <div class="">
+
            <p><img class="teamImgRight" width="300" src="https://static.igem.org/mediawiki/2015/2/20/China_Tongji_iGEM_logo.png" alt="" align="right"/></p>
+
            <p class="contentP">I am Songcheng Zhu, an Associate Professor at Life School of Tongji University.I like music, travelling, sports and stay with my kids in my spare time.My major is Biochemistry and I love those molecules in the body of life, particularly DNA, RNA and Protein. I am very interesting in how a protein is located to the right place in the the right time in a cell.</p>
+
            <p class="contentP">Happy to be a teacher in An University, where I can work and study with young students.Even more happy to work with our iGEM team, it is a diligent and enthusiastic unit I have ever seen.</p>
+
            <p class="contentP">Good luck to our team.</p>
+
      </div>
+
       
+
        <div class="divider"></div>
+
        <p class="titleTwo" id="third2">3.2 Wei Li</p>           
+
        <div class="">
+
            <p><img class="teamImgRight" width="300" src="https://static.igem.org/mediawiki/2015/2/20/China_Tongji_iGEM_logo.png" alt="" align="right"/></p>
+
            <p class="contentP">Dr. Li joined the faculty of the School of Life Sciences and Technology in 2012. Li received her Ph.D. degree in Biophysics from Huazhong University of Science and Technology. Prior to joining Tongji University, Li completed her post-doctoral training at the University of Michigan, where she was awarded the American Heart Association Postdoctoral Fellowship.</p>
+
            <p class="contentP">The Li lab combines the genetic model organism C.elegans and mammalian system to study the mechanisms of mechanosensation and pain, and uses a multidisciplinary approach of molecular genetics, functional imaging, electrophysiology, optogenetics and behavioral analysis.</p>
+
      </div>
+
       
+
        <div class="divider"></div>
+
  <p class="titleOne" id="Sponsor">4. Sponsor</p>
+
<div class="divider"></div>
+
        <p class="titleTwo" id="fourth1">4.1 GENEWIZ</p> 
+
 
         <p class="contentP">
 
         <p class="contentP">
         Excelling in both science and service, GENEWIZ is the largest trusted partner for DNA services. Since 1999, our commitment is to advance research productivity through our distinctive combination of fast, reliable results, specialized expertise, competitive pricing, excellent project management and easy access to award-winning, friendly technical support.</p>
+
         Cells sense the environment, process information, and make response to stimuli. To make cells work well in complex natural environments, lots of processes have to be preset to react to various signals. However, when well-characterized modules are combined to construct higher order systems, unpredictable behaviors often occur because of the interplay between modules. Another significant problem is that complex integrated systems composed of numerous parts may cause cell overload.</p>
 +
        <center><img class="contentImg" src="https://static.igem.org/mediawiki/2015/5/57/China_Tongji_team-logo-300px.png" ></center>
 +
        <p class="imgName" align="center">Figure 1. Schematic demonstration of HIV</p>
 
         <p class="contentP">
 
         <p class="contentP">
         With state-of-the-art facilities at the local, national, and international levels, we value our loyal customers who have made GENEWIZ an industry leader. Together, we can make a difference in the pursuit of scientific discoveries, better medicines, a greener environment, and abundant healthy food supplies.</p>
+
         We proposed an elegant method to design higher order systems. Instead of merely combining different functional modules, we constructed one integrated processing module with fewer parts by utilizing the common structures between modules. The circuit we designed is a rewirable one and the topological structure of the processing module can be altered to <span style="font-weight:bold;">adapt</span> to environmental change. The basic idea is to rewire the connections between parts and devices to <span style="font-weight:bold;">implement multiple functions</span> with the help of the site-specific recombination systems.</p>
 
         <p class="contentP">
 
         <p class="contentP">
         It's easy to work with GENEWIZ!</p>
+
         Our design approach may lead to a revolutionary step towards <span style="font-weight:bold;">system integration</span> in synthetic biology. Potential fields of application include organism development, living therapeutics and environment improvement.</p>
         <center><a href="http://www.genewiz.com" target="_blank">
+
         <p></p><div class="divider"></div>
        <img class="contentImg" width="800px" src="https://static.igem.org/mediawiki/2015/9/96/China-Tongji-Team-sponser-GENEWIZ.jpg" >
+
      </a></center>
+
  
<div class="divider"></div>
+
<p class="titleOne" id="Background">2. Background</p>             
         <p class="titleTwo" id="fourth2">4.2 THORLABS</p>
+
        <p class="contentP">Since its inception more than a decade ago, synthetic biology has undergone considerable development and has attained significant achievements with the help of the engineering slant. However, there are still obstacles to build a cell. Engineers try to abstract the DNA sequences into some standard functional parts and assemble them using some principles in electrical engineering. So far, the limited understanding of biological system prevents us to combine parts and modules to create larger scale systems. The complexity of synthetic systems didn’t increase rapidly as the Moore’s law (Purnick and Weiss, 2009). </p>
 +
        <p class="titleTwo" id="Challenges">2.1 Challenges</p>
 +
        <p class="contentP">There are some common problems that make the circuits we designed not work as our expected. Many failure modes have been collated by Brophy and Voigy in their review (Brophy and Voigt, 2014). In our project, we mainly focus on two modes, <span style="font-weight:bold;">crosstalk and host overload</span>, that emerge especially when we create more sophisticated systems. More specifically, regulators may interact with each other’s targets leading to errors in the desired operation, and the synthetic circuits may compete with natural parts that maintain the normal cellular processes for limited resources.</p>
 +
        <p class="titleTwo"  id="Solution">2.2 Solution</p>
 +
        <p class="contentP">We designed a time-sharing system that can process information according to the input signal. Cells rewire its synthetic circuit to <span style="font-weight:bold;">alter the topological structure of regulatory pathway</span> when they receive the corresponding stimuli. In this way, we <span style="font-weight:bold;">reuse the existing synthetic module</span> rather than add a new one to implement another function, which reduces the resource cost in running unnecessary function and prevents the interplay between parallel modules. After overcoming these two big problems, our engineered cells are more <span style="font-weight:bold;">versatile</span> and <span style="font-weight:bold;">flexible</span> in information processing. </p>
 +
        <p></p><div class="divider"></div>
 +
          
 +
<p class="titleOne" id="Design">3. Design</p>  
 
         <p class="contentP">
 
         <p class="contentP">
         Thorlabs, Inc. is a privately held optical equipment company headquartered in Newton, New Jersey. The company was founded by Alex Cable, who serves as its current president and CEO. As of 2013, Thorlabs employed about 1,000 people directly or indirectly and has sales of approximately $200 million.The company has offices in multiple American States, Brazil, Canada, China, France, Germany, Japan, Sweden, and the United Kingdom. It sells approximately 20,000 different products.</p>
+
         Cells sense the environment, process information, and make response to stimuli. To make cells work well in complex natural environments, lots of processes have to be preset to react to various signals. However, when well-characterized modules are combined to construct higher order systems, unpredictable behaviors often occur because of the interplay between modules. Another significant problem is that complex integrated systems composed of numerous parts may cause cell overload.</p>
 +
        <center><img class="contentImg" src="https://static.igem.org/mediawiki/2015/5/57/China_Tongji_team-logo-300px.png" ></center>
 +
        <p class="imgName" align="center">Figure 2. China Tongji logo</p>
 
         <p class="contentP">
 
         <p class="contentP">
         Thorlabsdesigns and manufactures products in the areas of breath analysis technology, fiber optics, lasers, optical instrumentation, optomechanics, photonics, and vibration isolation. Approximately 90% of the products it sells are manufactured in-house.</p>
+
         Our design approach may lead to a revolutionary step towards <span style="font-weight:bold;">system integration</span> in synthetic biology. Potential fields of application include organism development, living therapeutics and environment improvement.</p>
         <center><a href="http://www.thorlabs.com" target="_blank">
+
         <p></p><div class="divider"></div>
        <img class="contentImg" width="800px" src="https://static.igem.org/mediawiki/2015/f/f5/China-Tongji-Team-sponser-THORLABS.jpg" >
+
       
         </a></center>
+
<p class="titleOne" id="Protocol">4. Protocol</p> 
 
+
        <p class="contentP">
<div class="divider"></div>
+
        Cells sense the environment, process information, and make response to stimuli. To make cells work well in complex natural environments, lots of processes have to be preset to react to various signals. However, when well-characterized modules are combined to construct higher order systems, unpredictable behaviors often occur because of the interplay between modules. Another significant problem is that complex integrated systems composed of numerous parts may cause cell overload.</p>
         <p class="titleOne" id="Contact">5. Contact</p>   
+
        <p class="contentP">
 +
        Our design approach may lead to a revolutionary step towards <span style="font-weight:bold;">system integration</span> in synthetic biology. Potential fields of application include organism development, living therapeutics and environment improvement.</p>
 +
         <p></p><div class="divider"></div>
 +
       
 +
         <p class="titleOne" id="SummaryResult">5. Summary and Result</p>   
 
         <p class="contentP">
 
         <p class="contentP">
 
         Cells sense the environment, process information, and make response to stimuli. To make cells work well in complex natural environments, lots of processes have to be preset to react to various signals. However, when well-characterized modules are combined to construct higher order systems, unpredictable behaviors often occur because of the interplay between modules. Another significant problem is that complex integrated systems composed of numerous parts may cause cell overload.</p>
 
         Cells sense the environment, process information, and make response to stimuli. To make cells work well in complex natural environments, lots of processes have to be preset to react to various signals. However, when well-characterized modules are combined to construct higher order systems, unpredictable behaviors often occur because of the interplay between modules. Another significant problem is that complex integrated systems composed of numerous parts may cause cell overload.</p>
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         <p class="contentP">
 
         <p class="contentP">
 
         Our design approach may lead to a revolutionary step towards <span style="font-weight:bold;">system integration</span> in synthetic biology. Potential fields of application include organism development, living therapeutics and environment improvement.</p>
 
         Our design approach may lead to a revolutionary step towards <span style="font-weight:bold;">system integration</span> in synthetic biology. Potential fields of application include organism development, living therapeutics and environment improvement.</p>
 
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{{China_Tongji_Foot}}
 
{{China_Tongji_Foot}}

Revision as of 15:06, 26 August 2015

close label

Project

  • Overview

  • Background

    • Challenges
    • Solution
  • Design

  • Protocol

  • Summary and Result

  • 1. Overview

    Cells sense the environment, process information, and make response to stimuli. To make cells work well in complex natural environments, lots of processes have to be preset to react to various signals. However, when well-characterized modules are combined to construct higher order systems, unpredictable behaviors often occur because of the interplay between modules. Another significant problem is that complex integrated systems composed of numerous parts may cause cell overload.

    Figure 1. Schematic demonstration of HIV

    We proposed an elegant method to design higher order systems. Instead of merely combining different functional modules, we constructed one integrated processing module with fewer parts by utilizing the common structures between modules. The circuit we designed is a rewirable one and the topological structure of the processing module can be altered to adapt to environmental change. The basic idea is to rewire the connections between parts and devices to implement multiple functions with the help of the site-specific recombination systems.

    Our design approach may lead to a revolutionary step towards system integration in synthetic biology. Potential fields of application include organism development, living therapeutics and environment improvement.

    2. Background

    Since its inception more than a decade ago, synthetic biology has undergone considerable development and has attained significant achievements with the help of the engineering slant. However, there are still obstacles to build a cell. Engineers try to abstract the DNA sequences into some standard functional parts and assemble them using some principles in electrical engineering. So far, the limited understanding of biological system prevents us to combine parts and modules to create larger scale systems. The complexity of synthetic systems didn’t increase rapidly as the Moore’s law (Purnick and Weiss, 2009).

    2.1 Challenges

    There are some common problems that make the circuits we designed not work as our expected. Many failure modes have been collated by Brophy and Voigy in their review (Brophy and Voigt, 2014). In our project, we mainly focus on two modes, crosstalk and host overload, that emerge especially when we create more sophisticated systems. More specifically, regulators may interact with each other’s targets leading to errors in the desired operation, and the synthetic circuits may compete with natural parts that maintain the normal cellular processes for limited resources.

    2.2 Solution

    We designed a time-sharing system that can process information according to the input signal. Cells rewire its synthetic circuit to alter the topological structure of regulatory pathway when they receive the corresponding stimuli. In this way, we reuse the existing synthetic module rather than add a new one to implement another function, which reduces the resource cost in running unnecessary function and prevents the interplay between parallel modules. After overcoming these two big problems, our engineered cells are more versatile and flexible in information processing.

    3. Design

    Cells sense the environment, process information, and make response to stimuli. To make cells work well in complex natural environments, lots of processes have to be preset to react to various signals. However, when well-characterized modules are combined to construct higher order systems, unpredictable behaviors often occur because of the interplay between modules. Another significant problem is that complex integrated systems composed of numerous parts may cause cell overload.

    Figure 2. China Tongji logo

    Our design approach may lead to a revolutionary step towards system integration in synthetic biology. Potential fields of application include organism development, living therapeutics and environment improvement.

    4. Protocol

    Cells sense the environment, process information, and make response to stimuli. To make cells work well in complex natural environments, lots of processes have to be preset to react to various signals. However, when well-characterized modules are combined to construct higher order systems, unpredictable behaviors often occur because of the interplay between modules. Another significant problem is that complex integrated systems composed of numerous parts may cause cell overload.

    Our design approach may lead to a revolutionary step towards system integration in synthetic biology. Potential fields of application include organism development, living therapeutics and environment improvement.

    5. Summary and Result

    Cells sense the environment, process information, and make response to stimuli. To make cells work well in complex natural environments, lots of processes have to be preset to react to various signals. However, when well-characterized modules are combined to construct higher order systems, unpredictable behaviors often occur because of the interplay between modules. Another significant problem is that complex integrated systems composed of numerous parts may cause cell overload.

    Figure 2. China_Tongji_iGEM_logo

    Our design approach may lead to a revolutionary step towards system integration in synthetic biology. Potential fields of application include organism development, living therapeutics and environment improvement.