Difference between revisions of "Team:Sherbrooke/Practices"
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Revision as of 18:20, 1 September 2015
Human Practices
Feasability
Platform
State of the art
![](https://static.igem.org/mediawiki/2015/0/04/Sherbrooke_Rapid_hit_platform.png)
Figure 1 - «RapidHit» platform from Hudson Robotics
![](https://static.igem.org/mediawiki/2015/4/4f/Sherbrooke_EpMotion_5075_platform.png)
Figure 2 - «epMotion 5075v» from Eppendorf
![](https://static.igem.org/mediawiki/2015/6/69/Sherbrooke_OT.ONE_platform.png)
Figure 3 - OpenTrons OT.One platform
Specifications
Some specifications were taken in consideration during the designing process of the robotic platform. While respecting those specifications, the thought process behind the design kept as main focus to deliver a customizable platform, low cost but still effective. The principal specifications were:
- Movements of the robotic platform must have a 1 mm precision due to the narrowness of the micro plates’ spacing between wells
- Must be able to use different kinds of pipette tools at the same time and change tools with ease.
- Gripper must be precise enough, without putting excessive force, to grab as small as 1.5 mL tubes and as large as 96-well plates. It also needs to have a range of opening from 0 mm to 85 mm
- Centrifuge needs to rotate at a speed capable of exerting a minimum gravitational force of 6000G and must be equipped with security devices such as detection of abnormal vibration or securing the lid after closing
- Complete platform must be fully open-hardware and detailed at no more than 10000$
Specifications achieved
Some specifications have already been achieved (the others will be achieved by December 2015):
- Movements of the robotic platform must have a 1 mm precision
- Complete platform must be fully open-hardware and detailed at no more than 10000$
Approximate cost by parts
Parts | Cost(USD) |
---|---|
Platform frame’s pieces | 600$ |
Motor’s drive | 59$ |
Cable track | 53$ |
X/Y Motors | 65$ |
Gripper | 300$ |
Screw for the x/y axis | 250$ |
Single channel pipette (not include) | 100$ |
Multi-channel pipette (not include) | 300$ | Total | 1727$ |
Modules
State of the art
Actually, there are different robots available on the market that are capable of performing the tasks and the functions that are to be implemented by our modules. However, most of them are only capable of doing one specific task and this, at a significantly higher cost than what is desired for our project. As an example, Table 1 presents different options available on the market to regulate the temperature of a 96-well plate. It is to be noted that these options only control the temperature cycles, which represents only one of the capabilities implemented by our modules.
Table 1: Comparative Table of Temperature Cycling Module
Manufacturer | Product Name | Price(USD) | Temperature range(°C) | Temperature speed(°C/s) |
---|---|---|---|---|
Life Technologies | SimpliAmpTM Thermal Cycler | 4 995,00$ | 0 - 100°C ±0.25°C | 4°C/s |
OpenPCR | Open Source PCR Thermalcycler | 649,00$ | 10 - 105°C ±0.5°C | 1°C/s |
Eppendorf | Mastercycler® nexus | 7 200,00$ | 4-99°C ±0.2°C | 2.5°C/s |
Inheco | On Deck Thermal Cycle-ODTC 96 | On demand | 4-99°C ±0.3°C | 3.3°C/s |
Eppendorf | ThermoStat plus | Discontinued | 1-99°C ±1°C | 1.5°C/s |
1. OpenPCR cannot withstand a 96-well plate, but is able to cycle 1.5mL tubes. 2. ThermosStat plus is designed to accommodate either 24 x 1.5/2.0mL tubes or 8 x 50mL tubes or 1 x 96-well plate
Specifications