Difference between revisions of "Team:China Tongji/Achivement"
Line 291: | Line 291: | ||
<!--动态展示大图--> | <!--动态展示大图--> | ||
− | <div id="showPicBac"> | + | <!--<div id="showPicBac"> |
<div id="picAndWords"> | <div id="picAndWords"> | ||
<p><img id="bigImage" src="" alt=""/></p> | <p><img id="bigImage" src="" alt=""/></p> | ||
<p class="showPicDown"><img class="hoverHand" id="picClose" src="https://static.igem.org/mediawiki/2015/9/95/China_Tongji-PicClose.gif" alt="close label" align="right"/><p id="showPicTitle"></p></p> | <p class="showPicDown"><img class="hoverHand" id="picClose" src="https://static.igem.org/mediawiki/2015/9/95/China_Tongji-PicClose.gif" alt="close label" align="right"/><p id="showPicTitle"></p></p> | ||
</div> | </div> | ||
− | </div> | + | </div> --> |
<!-- Load javascript code --> | <!-- Load javascript code --> | ||
Line 356: | Line 356: | ||
} | } | ||
} | } | ||
− | + | // //大图展示 | |
− | + | // $('.bigImgShow').ready(function() { | |
− | + | // $('.bigImgShow').click(function(){ | |
− | + | // var picWidth = parseInt(this.title.substring(0,3)); //parseInt将字符串转换为数字 | |
− | + | // var picHeight = parseInt(this.title.substring(4)); | |
− | + | // var windowHeight = $(window).height(); | |
− | + | // var paddingTop = (windowHeight-picHeight-83)/2; | |
− | + | // $('#showPicBac').css("padding-top",paddingTop); | |
− | + | // $('#showPicBac').css("display","block"); | |
− | + | // $('#bigImage').attr("src",this.src); | |
− | + | // $('#bigImage').css("width",picWidth+"px"); | |
− | + | // $('#bigImage').css("height",picHeight+"px"); | |
− | + | // $('#showPicTitle').text(this.alt); | |
− | + | // $('#picAndWords').animate({width:picWidth},750); | |
− | + | // $('#picAndWords').animate({height:picHeight+43},300); | |
− | + | // }); | |
− | + | // }); | |
− | + | // //关闭大图展示 | |
− | + | // $('#picClose,#showPicBac').click(function(){ | |
− | + | // $('#showPicBac').css("display","none"); | |
− | + | // $('#picAndWords').css("width","100px"); | |
− | + | // $('#picAndWords').css("height","100px"); | |
− | + | // }); | |
//图片切换 | //图片切换 | ||
picMove($('#banner_tabsSecond2'),$('#bannerCtrlSecond2'),$('#banner_tabsSecond2 .flex-prev'),$('#banner_tabsSecond2 .flex-next')); | picMove($('#banner_tabsSecond2'),$('#bannerCtrlSecond2'),$('#banner_tabsSecond2 .flex-prev'),$('#banner_tabsSecond2 .flex-next')); | ||
picMove($('#banner_tabsSecond4'),$('#bannerCtrlSecond4'),$('#banner_tabsSecond4 .flex-prev'),$('#banner_tabsSecond4 .flex-next')); | picMove($('#banner_tabsSecond4'),$('#bannerCtrlSecond4'),$('#banner_tabsSecond4 .flex-prev'),$('#banner_tabsSecond4 .flex-next')); | ||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
}); | }); | ||
</script> | </script> |
Revision as of 19:22, 4 September 2015
![](https://static.igem.org/mediawiki/2015/6/6e/China-Tongji-achievementBug.png)
Achivement
1. Parts
2. Equipment
- 2.1 Introduction
- 2.2 The installation of light source
- 2.3 The adjustment of light path
- 2.4 The refit of the light sources
3. Modeling
4. Judging Form
1. Parts
Cells sense the environment, process information, and make response to stimuli. To make cells work well in complex natural environments, lots of processes have to be preset to react to various signals. However, when well-characterized modules are combined to construct higher order systems, unpredictable behaviors often occur because of the interplay between modules. Another significant problem is that complex integrated systems composed of numerous parts may cause cell overload.
![](https://static.igem.org/mediawiki/2015/5/57/China_Tongji_team-logo-300px.png)
Figure 1. Schematic demonstration of HIV
We proposed an elegant method to design higher order systems. Instead of merely combining different functional modules, we constructed one integrated processing module with fewer parts by utilizing the common structures between modules. The circuit we designed is a rewirable one and the topological structure of the processing module can be altered to adapt to environmental change. The basic idea is to rewire the connections between parts and devices to implement multiple functions with the help of the site-specific recombination systems.
Our design approach may lead to a revolutionary step towards system integration in synthetic biology. Potential fields of application include organism development, living therapeutics and environment improvement.
2. Equipment--Install our LED light Source
2.1 Introduction
ChR2 need a high power light to let it work on C.elegan. However, in our lab, we don’t have the light source which is powerful enough to let us use it for optogenetics. So we bought some parts from THORLABS, and prepare to build our own LED light source by ourselves.
Here are all the parts we need to build LED light source:
![500,540 ACL2520-A](https://static.igem.org/mediawiki/2015/b/b9/China-Tongji-Achievement-Equipment-ACL2520-A.jpg)
ACL2520-A
![500,540 B1CM](https://static.igem.org/mediawiki/2015/2/23/China-Tongji-Achievement-Equipment-B1CM.jpg)
B1CM
![500,540 B4CM](https://static.igem.org/mediawiki/2015/3/3d/China-Tongji-Achievement-Equipment-B4CM.jpg)
B4CM
![500,540 C4W](https://static.igem.org/mediawiki/2015/2/2a/China-Tongji-Achievement-Equipment-C4W.jpg)
C4W
![500,540 DC2100](https://static.igem.org/mediawiki/2015/d/d1/China-Tongji-Achievement-Equipment-DC2100.jpg)
DC2100
![500,540 DMLP550R](https://static.igem.org/mediawiki/2015/4/4a/China-Tongji-Achievement-Equipment-DMLP550R.jpg)
DMLP550R
![500,540 FFM1](https://static.igem.org/mediawiki/2015/4/40/China-Tongji-Achievement-Equipment-FFM1.jpg)
FFM1
![500,540 FL460-10](https://static.igem.org/mediawiki/2015/2/26/China-Tongji-Achievement-Equipment-FL460-10.jpg)
FL460-10
![500,540 FL532-10](https://static.igem.org/mediawiki/2015/f/f0/China-Tongji-Achievement-Equipment-FL532-10.jpg)
FL532-10
![500,540 FL560-10](https://static.igem.org/mediawiki/2015/2/24/China-Tongji-Achievement-Equipment-FL560-10.jpg)
FL560-10
![500,540 FL635-10](https://static.igem.org/mediawiki/2015/1/1e/China-Tongji-Achievement-Equipment-FL635-10.jpg)
FL635-10
![500,540 LEDD1B](https://static.igem.org/mediawiki/2015/a/a7/China-Tongji-Achievement-Equipment-LEDD1B.jpg)
LEDD1B
![500,540 M470L3](https://static.igem.org/mediawiki/2015/a/a7/China-Tongji-Achievement-Equipment-M470L3.jpg)
M470L3
![500,540 M530L3](https://static.igem.org/mediawiki/2015/7/75/China-Tongji-Achievement-Equipment-M530L3.jpg)
M530L3
![500,540 M590L3](https://static.igem.org/mediawiki/2015/3/30/China-Tongji-Achievement-Equipment-M590L3.jpg)
M590L3
![500,540 M625L3](https://static.igem.org/mediawiki/2015/0/04/China-Tongji-Achievement-Equipment-M625L3.jpg)
M625L3
![500,540 SM1A14](https://static.igem.org/mediawiki/2015/e/e3/China-Tongji-Achievement-Equipment-SM1A14.jpg)
SM1A14
![500,540 SM1CP2](https://static.igem.org/mediawiki/2015/b/b2/China-Tongji-Achievement-Equipment-SM1CP2.jpg)
SM1CP2
![500,540 SM1L03](https://static.igem.org/mediawiki/2015/5/50/China-Tongji-Achievement-Equipment-SM1L03.jpg)
SM1L03
![500,540 SM1T2](https://static.igem.org/mediawiki/2015/7/7c/China-Tongji-Achievement-Equipment-SM1T2.jpg)
SM1T2
![500,540 SM1V05](https://static.igem.org/mediawiki/2015/2/21/China-Tongji-Achievement-Equipment-SM1V05.jpg)
SM1V05
![500,540 TPS001](https://static.igem.org/mediawiki/2015/1/18/China-Tongji-Achievement-Equipment-TPS001.jpg)
TPS001
2.2 The installation of light source
(1) Put the proper light filter into SM1L03 and fix it by SM1RR, and then fix the SM1L03 containing its filter to its light source. After that fix ACL2520-A inside of SM1V05, then fix them in front of SM1L03. Link this packaged light source to the C4W cube by using SM1T2.
(2) Fix FFM1 to the B4C/M by using the prepared screws, then use FFM1 to nip the DMLP550R (please pay attention to the position which the filter located, the light whose wavelength is larger than 550nm will pass this filter, otherwise the light will be reflexed. When choosing the location of light sources, this matter should be considered as well). Then fixed the B4C/M to the cube carefully.
(3) The surface which fixed with B4C/M is the underside of the cube. Use B1C/M to seal up the top surface of the cube. Fix two proper light sources to the surfaces which lights come in of C4W. The surface which light comes out will links to the microscope by using SM1T2 and SM1A14. The remaining surface will blocked by SM1CP2.
(4) The installation has been completed.
2.3 The adjustment of light path
![500,500 Figure4](https://static.igem.org/mediawiki/2015/0/06/China-Tongji-Achievement-Equipment-pic4.jpg)
Figure4: We are adjusting the light path, the spot is clear and convergent, it means the light path has been collimated.
(1) The light path will be collimated by changing the length between light source and convex lens. When the length is changing, pay attention to the light spot on the optical screen. If the spot is clear and convergent, it means the light path has been collimated.
(2) After join the light sources to the C4W cube, the light spot can be changed by rotating B4C/M and the screws on B4C/M.
(3) The adjustment has been completed.
2.4 The refit of the light sources
After we build our LED light source, we found that it’s still not powerful enough for our experiment, so we decided to change the light source to 5W. we bought the 5W LED light from internet and replaced the old one.
(1) Take down the LED of the light sources by using welding gun. Replace them by the high power LED. Make sure the surfaces of the substrate and the radiator are parallel.
(2) The refit has been completed.
3. Modeling
Cells sense the environment, process information, and make response to stimuli. To make cells work well in complex natural environments, lots of processes have to be preset to react to various signals. However, when well-characterized modules are combined to construct higher order systems, unpredictable behaviors often occur because of the interplay between modules. Another significant problem is that complex integrated systems composed of numerous parts may cause cell overload.
![](https://static.igem.org/mediawiki/2015/5/57/China_Tongji_team-logo-300px.png)
Figure 2. China Tongji logo
Our design approach may lead to a revolutionary step towards system integration in synthetic biology. Potential fields of application include organism development, living therapeutics and environment improvement.
4. Judging Form
Cells sense the environment, process information, and make response to stimuli. To make cells work well in complex natural environments, lots of processes have to be preset to react to various signals. However, when well-characterized modules are combined to construct higher order systems, unpredictable behaviors often occur because of the interplay between modules. Another significant problem is that complex integrated systems composed of numerous parts may cause cell overload.
Cells sense the environment, process information, and make response to stimuli. To make cells work well in complex natural environments, lots of processes have to be preset to react to various signals. However, when well-characterized modules are combined to construct higher order systems, unpredictable behaviors often occur because of the interplay between modules. Another significant problem is that complex integrated systems composed of numerous parts may cause cell overload.
![](https://static.igem.org/mediawiki/2015/2/20/China_Tongji_iGEM_logo.png)
Figure 2. China_Tongji_iGEM_logo
Our design approach may lead to a revolutionary step towards system integration in synthetic biology. Potential fields of application include organism development, living therapeutics and environment improvement.
![](https://static.igem.org/mediawiki/2015/9/96/China-Tongji-Team-sponser-GENEWIZ.jpg)
![](https://static.igem.org/mediawiki/2015/f/f5/China-Tongji-Team-sponser-THORLABS.jpg)
Copyright © China_Tongji iGEM 2015 | Developer: LiJunWu