Difference between revisions of "Team:London Biohackspace/protocols/ecoli-tansformation"
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| − | 1. Prepare overnight cultures (for use ~8 hours later) | + | 1. <b>Prepare overnight cultures (for use ~8 hours later)</b><br/> |
1.1 Take 2 new/sterile 50ml falcon tubes and add to each 10ml of sterile LB broth (there is usually an autoclaved 1 L duran flask of LB in the fridge, use 20ml from that and transfer to tubes under the flow hood).<br/> | 1.1 Take 2 new/sterile 50ml falcon tubes and add to each 10ml of sterile LB broth (there is usually an autoclaved 1 L duran flask of LB in the fridge, use 20ml from that and transfer to tubes under the flow hood).<br/> | ||
1.2 Inoculate the 2 falcon tubes from DH5α stock using a flamed loop.<br/> | 1.2 Inoculate the 2 falcon tubes from DH5α stock using a flamed loop.<br/> | ||
Revision as of 14:32, 6 September 2015
1. Prepare overnight cultures (for use ~8 hours later)Protocols
Electroporation Transformation of E. coli DH5α
1.1 Take 2 new/sterile 50ml falcon tubes and add to each 10ml of sterile LB broth (there is usually an autoclaved 1 L duran flask of LB in the fridge, use 20ml from that and transfer to tubes under the flow hood).
1.2 Inoculate the 2 falcon tubes from DH5α stock using a flamed loop.
1.3 Incubate at 37 °C with shaking overnight, or for 8 hours.
