Difference between revisions of "Team:BroadRun-NorthernVA/Safety"

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<h2>Safety in iGEM</h2>
 
<h2>Safety in iGEM</h2>
  
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<p>Please visit <a href="https://2015.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>To insure the safety of our team and the world outside of our operations, we took many steps to negate the possibility of the biological destruction of humanity. Here are the steps we used:</p>
  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<p><b>Planning:</b>
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Our journey began within the walls of our cozy laboratory, our team spent hours planning the safest approach to our experiment. We decided to use a harmless strain of yeast to conduct our experiments on amylase.
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</p>
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<p><b>Lab Safety:</b>
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To negate the possibility of any physical injuries, proper lab conduct was followed at all times. These procedures included:
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</p>
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<ul>
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<li> Wearing gloves and goggles
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<li>Disinfecting the workspace
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<li>Following chemical safety procedures
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<li>Maintaining a neat and well organized work environment
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<li>Using bleach to sterilize unused cultures
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<li>Keeping food and drink out of the lab
  
  
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<h4>Safe Project Design</h4>
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<h4>Product Safety</h4>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<p>Before we allowed our product to leave the lab  we carefully tested for any adverse effects it could have on the environment. </p>
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<p>There are several options to minimize risks to the environment: </p>
 
<ul>
 
<ul>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Incorporate a kill switch after a certain number of generations, or activated by a certain compound, so that the genetically modified yeast cells will not have a chance adversely affect the environment in the event the cells escape the water system/lab
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<li>Choosing parts that will not harm humans / animals / plants</li>
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</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Minimize the risk that our genetically engineered yeast will escape into the environment by isolating the secreted amylase from the microbial culture, and only using the enzyme to treat the water.  The yeast itself, will never leave the lab.
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<li>Including an "induced lethality" or "kill-switch" device</li>
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</li>
 
</ul>
 
</ul>
  
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<h4>Safe Lab Work</h4>
 
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
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<h4>Safe Shipment</h4>
 
  
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
  
  
 
</div>
 
</div>
 
</html>
 
</html>

Revision as of 00:07, 8 September 2015

{{BroadRun-NorthernVA}}



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Safety in iGEM

To insure the safety of our team and the world outside of our operations, we took many steps to negate the possibility of the biological destruction of humanity. Here are the steps we used:

Planning: Our journey began within the walls of our cozy laboratory, our team spent hours planning the safest approach to our experiment. We decided to use a harmless strain of yeast to conduct our experiments on amylase.

Lab Safety: To negate the possibility of any physical injuries, proper lab conduct was followed at all times. These procedures included:

  • Wearing gloves and goggles
  • Disinfecting the workspace
  • Following chemical safety procedures
  • Maintaining a neat and well organized work environment
  • Using bleach to sterilize unused cultures
  • Keeping food and drink out of the lab

    Product Safety

    Before we allowed our product to leave the lab we carefully tested for any adverse effects it could have on the environment.

    There are several options to minimize risks to the environment:

    • Incorporate a kill switch after a certain number of generations, or activated by a certain compound, so that the genetically modified yeast cells will not have a chance adversely affect the environment in the event the cells escape the water system/lab
    • Minimize the risk that our genetically engineered yeast will escape into the environment by isolating the secreted amylase from the microbial culture, and only using the enzyme to treat the water. The yeast itself, will never leave the lab.