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        <div class="labtitle">Sequences for the Chromate-detector</div>
 
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          <p class="journal-summary-heading">Detecting Chromate</p>
 
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          <p class="journal-content">This set of biobricks is based on an operon of ochrobactrum tritici, conferring resistance against Cr(VI). The Beijing institute of Biotechnology already submitted a biobrick based on this operon in 2013, <a href="http://parts.igem.org/Part:BBa_K1058008" target="_blank">BBa_K1058008</a> (See Figure 1). We used this brick, broke it down to its constituent parts, and reassembled it in a different way (See Figure 2).</p>
 
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            <img src="https://static.igem.org/mediawiki/2015/a/a0/Plasmid_map_BBa_K1058008.png" alt="Figure1 - Plasmid map of BBa_K1058008." class="plasmidimg">
 
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            <figcaption>Figure 1: Plasmid map of <a href="http://parts.igem.org/Part:BBa_K1058008" target="_blank">BBa_K1058008</a><br> by BIT2013.</figcaption>
 
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            <img src="https://static.igem.org/mediawiki/2015/d/d4/Dundee15_plasmid_map_pChrGFP.png" alt="Figure2 - Plasmid map after reassembling parts." class="plasmidimg">
 
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            <img src="https://static.igem.org/mediawiki/2015/c/c1/Dundee15_plasmid_map_ChrB.png" alt="Figure2 - Plasmid map after reassembling parts." class="plasmidimg">
 
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            <figcaption> Figure 2: Plasmid maps of BBa_K1590006 (pChrGFP) and BBa_K1590009 (ChrB).<br>
 
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            These 2 biobricks are transformed into one cell in order to rebuild a chromate sensor.</figcaption>
 
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          <p><h3>Recommended reading:</h3>
 
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              <li><a href="http://jb.asm.org/content/190/21/6996.full" target="_blank">Branco R et al. The Chromate-Inducible chrBACF Operon from the Transposable Element TnOtChr Confers Resistance to Chromium(VI) and Superoxide. Journal of Bacteriology. 2008;190(21):6996-7003.</a></li>
 
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              <li><a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3543429/" target="_blank">Branco R et al. Highly Sensitive, Highly Specific Whole-Cell Bioreporters for the Detection of Chromate in Environmental Samples. PloS one. 2013;8(1):e54005.</a></li>
 
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                  <p>>GFPmut2<br>
 
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                  ATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGCGATGTTAATGGGCAAAAATTCTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGGAAGCTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGCGTATGGTCTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTACAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAAATGGAATACAACTATAACTCACATAATGTATACATCATGGCAGACAAACCAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTAAAGATGGAAGCGTTCAATTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCCAAAGATCCCAACGAAAAGAGAGATCACATGATCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAA</p>
 
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Revision as of 17:26, 7 September 2015

Dundee iGEM 2015

Sequences and Parts

Registry - ID

Name

Short Description

Part of which tool

Sequences

BBa_K1590000

Haemoglobin A (Homo sapiens)

Coding sequence for Human Haemoglobin A in silico optimised for increased expression level in E.coli K12.

BioSpray - Blood Detection

Fasta Sequence

Primers

BBa_K1590001

Haemoglobin B (Homo sapiens)

Coding sequence for Human Haemoglobin A in silico optimised for increased expression level in E.coli K12.

BioSpray - Blood Detection

Fasta Sequence

Primers

BBa_K1590002

Haptoglobin (Homo sapiens)

Coding sequence for Human Haemoglobin A in silico optimised for increased expression level in E.coli K12.

BioSpray - Blood Detection

Fasta Sequence

Primers

BBa_K1058008 (BIT-2013)

pChr-ChrB-GFP

Partial sequence of chromate resistance operon of Ochrobactrum tritici 5bvl1, containing a chromate-inducible promoter, pChr, a repressor, ChrB, and a fluorescen reporter, GFP.

Chromate Detection

Fasta Sequence

Primers

BBa_K1590003

pChr

Promoter sequence of chromate resistance operon of Ochrobactrum tritici 5bvl1.

Chromate Detection

Fasta Sequence

Primers

-

ChrB

Coding sequence for unmodified chromate sensitive repressor of pChr.

Chromate Detection

Fasta Sequence

Primers

BBa_K1590004

ChrBopt

Coding sequence for partially optimised chromate sensitive repressor of pChr.

Chromate Detection

Fasta Sequence

Primers

BBa_K1590005

pChrGFP

Sequence of fluorescent reporter-fusion, chromate sensitive promoter + GFP.

Chromate Detection

Fasta Sequence

Primers

BBa_K1590006

Lanosterol Synthase (Homo Sapiens)

Coding sequence for Human lanosterol synthase in silico optimised for increased expression level in E. coli K12.

Fingerprint Ageing

Fasta Sequence

Primers

BBa_K1590007

Odorant Binding Protein 2A (Homo sapiens)

Coding sequence for human odorant binding 2A protein in silico optimised for increased expression level in E. coli K12.

BioSpray - Nasal Mucus Detection

Fasta Sequence

Primers

-

Odorant Binding Protein (Homo sapiens) - Helix

Coding sequence for C-terminal helix of human odorant binding protein.

BioSpray - Nasal Mucus Detection

Fasta Sequence

Primers

-

Odorant Binding Protein (Homo sapiens) - Barrel

Coding sequence for N-terminal barrel of human odorant binding protein.

BioSpray - Nasal Mucus Detection

Fasta Sequence

Primers

BBa_K1590008

Lactoferrin Binding Protein (Neisseria Meningitidis)

Coding sequence lactoferrin binding protein.

BioSpray - Saliva Detection

Fasta Sequence

Primers

BBa_K1590009

PotD (Escherichia Coli)

Unmodified coding sequence for E.coli Spermidine Binding Protein PotD.

BioSpray - Semen Detection

Fasta Sequence

Primers

BBa_K1590010

Spermine Binding Protein (Mus Musculus)

Coding sequence for murine Spermine Binding Protein in silico optimised for increased expression level in E. coli K12.

BioSpray - Semen Detection

Fasta Sequence

Primers