Difference between revisions of "Team:Hong Kong-CUHK/wetlabjournal"
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− | March | + | <h3> March </h3> |
− | 1. Learning Lab techniques and protocols | + | <p> 1. Learning Lab techniques and protocols </p> |
− | 2. Brainstorming ideas | + | <p> 2. Brainstorming ideas </p> |
− | 3. Researches | + | <p> 3. Researches </p> |
− | April | + | <h3> April </h3> |
− | 1. Project decided | + | <p> 1. Project decided |
− | 2. Preparation of special medium and plate for A. vinelandii | + | <p> 2. Preparation of special medium and plate for A. vinelandii </p> |
− | 3. Study the growth curve characteristics of A. vinelandii | + | <p> 3. Study the growth curve characteristics of A. vinelandii </p> |
− | 4. Preparation of Competent Cells of dh5α and BL21, aliquot the competent cells, stored in liquid nitrogen and then -80C refrigerator. | + | <p> 4. Preparation of Competent Cells of dh5α and BL21, aliquot the competent cells, stored in liquid nitrogen and then -80C refrigerator. </p> |
− | 5. Make plate with antibiotics -Ampicillin, kanamycin and chloramphenicol. | + | <p> 5. Make plate with antibiotics -Ampicillin, kanamycin and chloramphenicol. </p> |
− | 6. Make LB brooth | + | <p> 6. Make LB brooth </p> |
− | May | + | <h3> May </h3> |
− | 1. Purchase for primers for the magnetosome project. | + | <p> 1. Purchase for primers for the magnetosome project. </p> |
− | 2. Primer dilution of the all arrived primers | + | <p> 2. Primer dilution of the all arrived primers </p> |
− | 3. Amplification by using PCR, following by run gel of A. vinelandii ___ gene. | + | <p> 3. Amplification by using PCR, following by run gel of A. vinelandii ___ gene. </p> |
− | 4. Make plate with antibiotics -Ampicillin, kanamycin and chloramphenicol | + | <p> 4. Make plate with antibiotics -Ampicillin, kanamycin and chloramphenicol </p> |
− | 5. Make LB brooth | + | <p> 5. Make LB brooth </p> |
− | June | + | <h3> June </h3> |
− | 1. Amplification by using PCR, following by run gel of A. vinelandii ___ gene. | + | <p> 1. Amplification by using PCR, following by run gel of A. vinelandii ___ gene. </p> |
− | 2. Restriction of PCR product ______ , ligase into C backbone and transform into dh5α | + | <p> 2. Restriction of PCR product ______ , ligase into C backbone and transform into dh5α </p> |
− | 3. Make plate with antibiotics -Ampicillin, kanamycin and chloramphenicol | + | <p> 3. Make plate with antibiotics -Ampicillin, kanamycin and chloramphenicol </p> |
− | 4. Make LB brooth | + | <p> 4. Make LB brooth </p> |
− | July | + | <h3> July </h3> |
− | 1. Amplification by using PCR, following by run gel of A. vinelandii ___ gene. | + | <p> 1. Amplification by using PCR, following by run gel of A. vinelandii ___ gene. </p> |
− | 2. Restriction of PCR product ______ , ligase into C backbone and transform into dh5α | + | <p> 2. Restriction of PCR product ______ , ligase into C backbone and transform into dh5α </p> |
− | 3. Preparation of Competent Cells of dh5α and BL21, aliquot the competent cells, stored in liquid nitrogen and then -80C refrigerator. | + | <p> 3. Preparation of Competent Cells of dh5α and BL21, aliquot the competent cells, stored in liquid nitrogen and then -80C refrigerator. </p> |
− | 4. Make plate with antibiotics -Ampicillin, kanamycin and chloramphenicol | + | <p> 4. Make plate with antibiotics -Ampicillin, kanamycin and chloramphenicol </p> |
− | 5. Make LB brooth | + | <p> 5. Make LB brooth </p> |
− | August | + | <h3> August </h3> |
− | 1. Preparation of Competent Cells of A. vinelandii | + | <p> 1. Preparation of Competent Cells of A. vinelandii </p> |
− | 2. Amplification by using PCR, following by run gel of A. vinelandii ___ gene. | + | <p> 2. Amplification by using PCR, following by run gel of A. vinelandii ___ gene. </p> |
− | 3. Overlapping PCR of A. vinelandii nifB gene, A. vinelandii nifQ gene and A. vinelandii FdxA gene | + | <p> 3. Overlapping PCR of A. vinelandii nifB gene, A. vinelandii nifQ gene and A. vinelandii FdxA gene </p> |
− | 4. Restriction of PCR product ______ , ligase into C backbone and transform into dh5α. | + | <p> 4. Restriction of PCR product ______ , ligase into C backbone and transform into dh5α. </p> |
− | 5. Make plate with antibiotics -Ampicillin, kanamycin and chloramphenicol | + | <p> 5. Make plate with antibiotics -Ampicillin, kanamycin and chloramphenicol </p> |
− | 6. Make LB brooth | + | <p> 6. Make LB brooth </p> |
− | September | + | <h3> September </h3> |
− | 1. Amplification by using PCR, following by run gel of A. vinelandii ___ gene. | + | <p> 1. Amplification by using PCR, following by run gel of A. vinelandii ___ gene. </p> |
− | 2. Restriction of PCR product ______ , ligase into C backbone and transform into dh5α | + | <p> 2. Restriction of PCR product ______ , ligase into C backbone and transform into dh5α </p> |
− | 3. Make plate with antibiotics -Ampicillin, kanamycin and chloramphenicol | + | <p> 3. Make plate with antibiotics -Ampicillin, kanamycin and chloramphenicol </p> |
− | 4. Make LB brooth | + | <p> 4. Make LB brooth </p> |
− | 5. Submission of all biobricks to the registry | + | <p> 5. Submission of all biobricks to the registry </p> |
Latest revision as of 05:15, 13 September 2015