Difference between revisions of "Team:Bielefeld-CeBiTec/Results"
m |
|||
| Line 25: | Line 25: | ||
<div class="featurette"> | <div class="featurette"> | ||
<img class="featurette-image img-responsive pull-right" src="data:image/gif;base64,R0lGODlhAQABAIAAAHd3dwAAACH5BAAAAAAALAAAAAABAAEAAAICRAEAOw==" alt="survey result" width="500px" style="height: 400px"> | <img class="featurette-image img-responsive pull-right" src="data:image/gif;base64,R0lGODlhAQABAIAAAHd3dwAAACH5BAAAAAAALAAAAAABAAEAAAICRAEAOw==" alt="survey result" width="500px" style="height: 400px"> | ||
| − | <p class="lead">PRIA | + | <p class="lead"><p>We were able to establish a new assay called the <b>plasmid repressor interaction assay (PRIA)</b> for the model system LacI-<i>lacO</i>. |
| + | For the implementation of the assay on paper we immobilized amino- and Cy3-labeled DNA containing the operator site successfully on filter paper. We were able to prove with an electrophoretic mobility shift assay that the repressor proteins (ArsR, LacI and BlcR) fused with sfGFP we used can bind to oligonucleotides with the corresponding operator site (<i>arsO, lacO, P<sub>blc</sub></i>). For the detection of the fusion protein and Cy3-labeled DNA we were able to exhibit fluorescence of them both with the Ettan DIGE scanner.</p></p> | ||
</div> | </div> | ||
Revision as of 10:27, 13 September 2015
Results
Here you can find an overview of our results.
CFPS ist toll
We were able to establish a new assay called the plasmid repressor interaction assay (PRIA) for the model system LacI-lacO. For the implementation of the assay on paper we immobilized amino- and Cy3-labeled DNA containing the operator site successfully on filter paper. We were able to prove with an electrophoretic mobility shift assay that the repressor proteins (ArsR, LacI and BlcR) fused with sfGFP we used can bind to oligonucleotides with the corresponding operator site (arsO, lacO, Pblc). For the detection of the fusion protein and Cy3-labeled DNA we were able to exhibit fluorescence of them both with the Ettan DIGE scanner.
Weisen Sie ganz viele Schwermetalle auf einmal nach!
Und sogar K.O.-Tropfen
Die Auswertung geht ganz einfach mit dem Handy
Und modelliert haben wir das Ganze auch
Schauen Sie sich unsere BioBricks an