Difference between revisions of "Team:Marburg/Labbook/CDI"
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<table id="layouttable" style="border:2px;border-style:solid;border-color:#F0F0F0;"> | <table id="layouttable" style="border:2px;border-style:solid;border-color:#F0F0F0;"> | ||
<tr style="background-color:#f66300;"> | <tr style="background-color:#f66300;"> | ||
| − | <td>PCR Mix </td> | + | <td>PCR </td> |
| − | <td> | + | <td>PCR Mix 1 </td> |
| + | <td>PCR Mix 2</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class='col'> | + | <td class='col'>GXL buffer</td> |
| − | <td class='col'> | + | <td class='col'>10 uL </td> |
| + | <td class='col'>10 uL </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class='col'> | + | <td class='col'>dNTPs (from GXL kit</td> |
| − | <td class='col'> | + | <td class='col'>4 uL</td> |
| − | + | <td class='col'>4 uL</td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | |||
<td class='col'>GXL polymerase</td> | <td class='col'>GXL polymerase</td> | ||
| − | + | <td class='col'>2uL</td> | |
| + | <td class='col'>2uL</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | |||
<td class='col'>ICD001 (10 uM)</td> | <td class='col'>ICD001 (10 uM)</td> | ||
| − | + | <td class='col'>0.5uL</td> | |
| + | <td class='col'>1uL</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | |||
<td class='col'>ICD007/ICD008 (10 uM)</td> | <td class='col'>ICD007/ICD008 (10 uM)</td> | ||
| + | <td class='col'>0.5uL</td> | ||
| + | <td class='col'>1uL</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | |||
<td class='col'>EC93 genomic DNA (15 ng/uL)</td> | <td class='col'>EC93 genomic DNA (15 ng/uL)</td> | ||
| − | + | <td class='col'>0.5 uL</td> | |
| + | <td class='col'>1 uL</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | |||
<td class='col'>ddH2O</td> | <td class='col'>ddH2O</td> | ||
| − | + | <td class='col'>32.5uL</td> | |
| + | <td class='col'>29.5uL</td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
| + | </PCR program> | ||
| − | + | <table id="layouttable" style="border:2px;border-style:solid;border-color:#F0F0F0;"> | |
| + | <tr style="background-color:#f66300;"> | ||
| + | <td>Start cycle (30x) </td> | ||
| + | <td> class='col'> </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td class='col'>98 °C </td> | ||
| + | <td class='col'>10 s </td> | ||
| + | |||
| + | </tr> | ||
| + | <tr> | ||
| + | <td class='col'>60 °C</td> | ||
| + | <td class='col'>15 s</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td class='col'>68 °C</td> | ||
| + | <td class='col'>2 min</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td class='col'>close cycle</td> | ||
| + | <td class='col'> </td> | ||
| + | |||
| + | </tr> | ||
| + | <tr> | ||
| + | <td class='col'> 8 °C</td> | ||
| + | <td class='col'>store</td> | ||
| + | |||
| + | </tr> | ||
| + | |||
| + | </table> | ||
Revision as of 18:39, 12 September 2015
15/05/18
PCR
| PCR | PCR Mix 1 | PCR Mix 2 |
| GXL buffer | 10 uL | 10 uL |
| dNTPs (from GXL kit | 4 uL | 4 uL |
| GXL polymerase | 2uL | 2uL |
| ICD001 (10 uM) | 0.5uL | 1uL |
| ICD007/ICD008 (10 uM) | 0.5uL | 1uL |
| EC93 genomic DNA (15 ng/uL) | 0.5 uL | 1 uL |
| ddH2O | 32.5uL | 29.5uL |
| Start cycle (30x) | class='col'> |
| 98 °C | 10 s |
| 60 °C | 15 s |
| 68 °C | 2 min |
| close cycle | |
| 8 °C | store |
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