Difference between revisions of "Team:SPSingapore/Notebook-Week-2"

Line 130: Line 130:
  
 
<tr class = "tablenotebook"><td><p class = "paper">
 
<tr class = "tablenotebook"><td><p class = "paper">
−
31st May 2015
+
May 31
 
<br><br>
 
<br><br>
 
<strong>Xin Yi</strong>
 
<strong>Xin Yi</strong>
Line 141: Line 141:
  
 
<tr class = "tablenotebook"><td><p class = "paper">
 
<tr class = "tablenotebook"><td><p class = "paper">
−
2nd June 2015
+
June 2
 
<br><br>
 
<br><br>
 
<strong>Yi Han</strong>
 
<strong>Yi Han</strong>
Line 154: Line 154:
  
 
<tr class = "tablenotebook"><td><p class = "paper">
 
<tr class = "tablenotebook"><td><p class = "paper">
−
3rd June 2015
+
June 3
 
<br><br>
 
<br><br>
 
<strong>Yi Han</strong>
 
<strong>Yi Han</strong>
Line 166: Line 166:
  
 
<tr class = "tablenotebook"><td><p class = "paper">
 
<tr class = "tablenotebook"><td><p class = "paper">
−
4th June 2015
+
June 4
 
<br><br>
 
<br><br>
 
<strong>Chi Yan</strong>
 
<strong>Chi Yan</strong>
Line 177: Line 177:
  
 
<tr class = "tablenotebook"><td><p class = "paper">
 
<tr class = "tablenotebook"><td><p class = "paper">
−
5th June 2015
+
June 5
 
<br><br>
 
<br><br>
 
<strong>Yan Ting</strong>
 
<strong>Yan Ting</strong>
Line 190: Line 190:
  
 
<tr class = "tablenotebook"><td><p class = "paper">
 
<tr class = "tablenotebook"><td><p class = "paper">
−
6th June 2015
+
June 6
 
<br><br>
 
<br><br>
 
<strong>Yi Han & Yan Ting</strong>
 
<strong>Yi Han & Yan Ting</strong>

Revision as of 21:24, 12 September 2015


Research Notebook

Week 2 (31/5 - 6/6)

May 31

Xin Yi

• Sent EsaR clone 4 and INv-4 for sequencing.

June 2

Yi Han

• YFP and GFP transformation results - no colonies for YFP
• The GFP transformation repeated with 100ng of plasmid was sucessful.
• 4 colonies of gfp plasmid were inoculated in 3mL LB+amp and grown overnight.

June 3

Yi Han

• Miniprep of gfp plasmids
• RE digest with EcoRI and RsaI

June 4

Chi Yan

• RE digest indicated a very faint smaller band for colonies 2-4, and hence these were likely to be positive clones

June 5

Yan Ting

• Inoculated 3mLS of Inv-4 and EsaR-4 plasmid carrying bacteria into 100mL LB+ appropriate Antibiotic
• Cell culture → HEK293 cells revived from freezing down appeared detached.

June 6

Yi Han & Yan Ting

• Storage of bacterial glycerol stocks for Inv-4, EsaR4 in 25% glycerol
• Yan Ting: grew HEK293T cells in T25 glask
• Gel extract to clean up gfp plasmids which loading dye had accidentally been added to.