We helped Nagahama

We were helped by Nagahama

From their past experiences in 2014, iGEM Team Nahahama gave us these 2 advices about chemotaxis experiments.
    1. In order to increase the chemotactic activity of E. coli, the best concentration of agar is 0.3 %.
    2. It is better to directly stick the chip inside the agar and then inject the E .coli, than to place the E. coli on top of the agar.

Also, Team Nagahama gave us the raw data along with the protocol of the experiments for assaying the chemotactic activity of a wild type E.coli, following the protocol written above.

Fig. 7-4-3-1.Positive Chemotaxis of E.coli L…Center of plate：E. coli (JM109)、Right：Aspartic acid (10 mM, 40 microL) R…Center of plate：E.coli (JM109)、Right： Sterile water (40 microL) Incubation Time: 108 hours Temperature : 30 ℃

[[File:RhlR cmRssrA Assay Construction.png|200px|thumb|left|alt text]] [[File:Titech2013_M13_PlacIq_B.jpg|600px|thumb|center|Fig. 3-3-3. A mixture of only 3.5 mL YT soft agar and 500 µL of overnight culture of JM109 was poured on a YT plate.]]

Our plan at first was to integrate the chemotaxis experiment into our overall project. Unfortunately, we were unable to obtain positive results from the gene that we constructed, before the Giant Jamboree. However, the advice we received from Team Nagahama helped us a lot. We would like to say thanks to all the help we received from them.

May Festival

The Japanese iGEM teams took part in the school festival at the University of Tokyo. Our teams shared ideas and gave each other feedbacks. Together, we introduced synthetic biology to the public.
See the “Policy & Practices” page for further information.

Meetup with the iGEM LZU-China Team

In this event, we each introduced our project along with the project from our previous year. We exchanged ideas about each other’s project and gave each other feedbacks. The iGEM LZU-China team also participated in our “Prisoner’s Dilemma Experiment” that we conducted. This experiment is a sociological experiment concerning the safety of genetic modification and is part of our “Policy & Practices”. See the “Policy & Practices” page for further information.

Fig7-4-3-1 Meetup with the iGEM LZU-China Team

Meetup at Boston University

On September 23th, our team is going to host a meetup in Boston University. We are looking forward to sharing ideas with other iGEM teams. Teams will have the opportunity to present their projects and exchange their ideas in details with other iGEM teams and faculty advisors right before the Giant Jamboree.
Meetup page: https://2015.igem.org/Meetups/Tokyo_Tech_Sept