Difference between revisions of "Team:HZAU-China/InterLab"

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       <h1></br></br></br>Overview</h1></br>
 
       <h1></br></br></br>Overview</h1></br>
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       <p>As our team is competing this year's competition, we also required to participate in the iGEM 2015 Measurement Interlab study. The goal of the Interlab study is to obtain fluorescence data for three specific genetic devices expressing GFP from iGEM teams around world.</p>
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       <p>This year, we are not only in general event but also participate in Measurement Interlab. All the iGEM teams that are in this Interlab Study should collect fluorescence intensity data of three specific genetic devices which  is the same with others and can express GFP protein.</p>
 
       </br><p>The three genetic devices differ in their promotors :</p>
 
       </br><p>The three genetic devices differ in their promotors :</p>
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       <p><strong>Device 1: J23101 + I13504 (B0034-E0040-B0015), must be built in the pSB1C3 backbone</strong></p>
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       <p><strong>Device 1: J23101 + I13504 (B0034-E0040-B0015), in the pSB1C3 backbone</strong></p>
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       <p><strong>Device 2: J23106 + I13504 (B0034-E0040-B0015), must be built in the pSB1C3 backbone</strong></p>
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       <p><strong>Device 2: J23106 + I13504 (B0034-E0040-B0015), in the pSB1C3 backbone</strong></p>
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       <p><strong>Device 3: J23117 + I13504 (B0034-E0040-B0015), must be built in the pSB1C3 backbone</strong></p></br>
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       <p><strong>Device 3: J23117 + I13504 (B0034-E0040-B0015), in the pSB1C3 backbone</strong></p></br>
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       <p>We transformed the three devices into competent cell (DH 5 alpha) and measured fluorescence as well as optical density of the LB medium at the suitable period (We did pre-experiment before formal experiment for confirming the suitable period as well as the dilution factor), using a plate reader, respectively. We hypothesized that the GFP expression quality of the three devices level is Device 1, Device 2, Device 3, respectively. And our final result accord with our hypothesis.</p>
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       <p>Firstly, we transformed these three devices into competent cells (DH 5 alpha) respectively. And then, We did pre-experiment before formal experiment to confirm the suitable period as well as the dilution factor. What’s more, their fluorescence intensity is measured with a plate reader in suitable optical density of the LB medium. We assumed that the GFP expression quality of them ordered from high to low would be Device 1, Device 2, Device 3. Finally, the result confirmed our conjecture.</p>
 
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Revision as of 02:49, 16 September 2015

Mixed-Reality Cell

Bidirectinal coupling between real and virtual bio-oscillator




Overview


This year, we are not only in general event but also participate in Measurement Interlab. All the iGEM teams that are in this Interlab Study should collect fluorescence intensity data of three specific genetic devices which is the same with others and can express GFP protein.


The three genetic devices differ in their promotors :

Device 1: J23101 + I13504 (B0034-E0040-B0015), in the pSB1C3 backbone

Device 2: J23106 + I13504 (B0034-E0040-B0015), in the pSB1C3 backbone

Device 3: J23117 + I13504 (B0034-E0040-B0015), in the pSB1C3 backbone


Firstly, we transformed these three devices into competent cells (DH 5 alpha) respectively. And then, We did pre-experiment before formal experiment to confirm the suitable period as well as the dilution factor. What’s more, their fluorescence intensity is measured with a plate reader in suitable optical density of the LB medium. We assumed that the GFP expression quality of them ordered from high to low would be Device 1, Device 2, Device 3. Finally, the result confirmed our conjecture.




© 2015 Huazhong Agricultural University iGEM Team. All rights reserved.

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    430070,P.R.China
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