Difference between revisions of "Team:Marburg/iGeneration"
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<div style="z-index:1;text-align:center;padding-bottom:30px;"><img src="https://static.igem.org/mediawiki/2015/e/ed/MR_pic_Button_iGeneration.png" style="height:60px;"/></div> | <div style="z-index:1;text-align:center;padding-bottom:30px;"><img src="https://static.igem.org/mediawiki/2015/e/ed/MR_pic_Button_iGeneration.png" style="height:60px;"/></div> | ||
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| + | <div style="position:relative;display:inline-block;padding:15px;background:#F8F8F8;border-style:solid;border-color:#FF8F45;border-width:2px;border-radius:10px;text-align:justify;font-size:13pt;line-height:150%;margin:10px;"> | ||
| + | <h1>Aim</h1> | ||
| + | <p>This year, the iGEM Team Marburg is participating together with many other teams in the InterLab Study. The aim of the InterLab Study is to investigating the reproducibility of BioBrick characterization by collecting data of the same experiment from iGEM Teams all around the globe. All teams are measuring the fluorescence of three different genetic devices constitutively expressing GFP who differ in their promoter strength. These data will allow a comparison of different protocols and lab techniques regarding the variety of the three parts. | ||
| + | We transformed the three devices into E. coli cells, diluted to an optical density (OD) of 0.5 at 600 nm using both, a spectrophotometer and a plate reader and measured the fluorescence with a plate reader. The obtained results showed that the promoter J23101 was the strongest one, the promoter J23106 was weaker and the devices with promoter J23117 showed barely fluorescence over the background.</p> | ||
| + | </div> | ||
<h1>TEXT</h1> | <h1>TEXT</h1> | ||
Revision as of 12:09, 16 September 2015
Aim
This year, the iGEM Team Marburg is participating together with many other teams in the InterLab Study. The aim of the InterLab Study is to investigating the reproducibility of BioBrick characterization by collecting data of the same experiment from iGEM Teams all around the globe. All teams are measuring the fluorescence of three different genetic devices constitutively expressing GFP who differ in their promoter strength. These data will allow a comparison of different protocols and lab techniques regarding the variety of the three parts. We transformed the three devices into E. coli cells, diluted to an optical density (OD) of 0.5 at 600 nm using both, a spectrophotometer and a plate reader and measured the fluorescence with a plate reader. The obtained results showed that the promoter J23101 was the strongest one, the promoter J23106 was weaker and the devices with promoter J23117 showed barely fluorescence over the background.
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