Difference between revisions of "Team:Waterloo"
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| − | + | <div class="small-cover col-sm-6" id="desc"><a href="/Team:Waterloo/Description">Description</a></div> | |
| − | + | <div class="small-cover col-sm-6" id="medal"><a href="/Team:Waterloo/Requirements">Medal Requirements</a></div> | |
| − | + | <div class="small-cover col-sm-4" id="lab"><a href="/Team:Waterloo/Lab_Overview">Lab & Design</a></div> | |
| − | + | <div class="small-cover col-sm-4" id="math"><a href="/Team:Waterloo/Modeling">Math Modeling</a></div> | |
| − | + | <div class="small-cover col-sm-4" id="policy"><a href="/Team:Waterloo/Practices">Policy & Practices</a></div> | |
| − | + | <div class="small-cover col-sm-4" id="team"><a href="/Team:Waterloo/Team">Team</a></div> | |
| + | <div class="small-cover col-sm-4" id="att"><a href="/Team:Waterloo/Attributions">Attributions</a></div> | ||
| + | <div class="small-cover col-sm-4" id="collab"><a href="/Team:Waterloo/Collaborations">Collaborations</a></div> | ||
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Revision as of 23:00, 15 September 2015
Swappable sgRNA Targets
Engineered PAM Flexibility
Antiviral Protection for Plants
Re-engineering CRISPR-Cas9 with functional applications in eukaryotic systems
CRISPR-Cas9 is an exciting tool for synthetic biologists because it can target and edit genomes with unprecedented specificity. Our team is attempting to re-engineer CRISPR to make it more flexible and easier to use.
We’re making it easy to test different sgRNA designs: restriction sites added to the sgRNA backbone allow 20 nucleotide target sequences to be swapped without excessive cloning.
Additionally, we’re applying recent research on viable mutations within Cas9’s PAM-interacting domain to design (d)Cas9 variants that bind to novel PAM sites, moving towards the goal of a suite of variants that can bind any desired sequence. We believe our re-engineered CRISPR-Cas9 will give biologists increased ability to optimize targeting in many applications.
The application we chose to explore is a proof-of-concept antiviral system defending the model plant Arabidopsis thaliana against Cauliflower Mosaic Virus, which would benefit from testing a large number of possible sgRNAs in the viral genome.