Difference between revisions of "Team:elan vital korea/safety"
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+ | <a href="#top" rel="" id="top" class="anchorLink"><img class="displayed" src="https://static.igem.org/mediawiki/2015/b/b3/Scroll_arrow_top.PNG"></a> | ||
+ | <h6 style="text-align:center;"> <font color="white"> | ||
+ | To The Top | ||
+ | </font> </h6> | ||
+ | </section> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <!-- Section #5 --> | ||
+ | <section id="maintext2" data-speed="10" data-type="background"> | ||
+ | <br> | ||
+ | |||
+ | |||
+ | |||
+ | <div class="inner3"> | ||
+ | <font color="white"> | ||
+ | <div class="caption4"> | ||
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<font color="white"> 19 </font> | <font color="white"> 19 </font> | ||
</h5> | </h5> | ||
<p> | <p> | ||
− | + | Person who leaves the lab last must make sure that power is disconnected, flammable material is isolated, dangerous material is safely kept, water is disconnected, lab is cleaned and locked, and order of the lab must be double- checked. | |
</p> | </p> | ||
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− | <div class=" | + | <div class="caption4"> |
+ | <h5> | ||
+ | <font color="white"> 19 </font> | ||
+ | </h5> | ||
+ | <p> | ||
+ | Power wiring appliance must be properly installed to accommodate the experiments. Cables, and lines must be checked regularly to confirm that they are capable of handling the electrical load. | ||
+ | </p> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="caption4"> | ||
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<font color="white"> 20 </font> | <font color="white"> 20 </font> | ||
</h5> | </h5> | ||
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− | + | All equipment must be connected with the proper power Cable and must not be connected in a multiple power socket. | |
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− | + | Autoclave for 20 min within 2 hr | |
</p> | </p> | ||
</div> | </div> | ||
+ | <div class="caption3"> | ||
+ | <h5> | ||
+ | <font color="white"> 22 </font> | ||
+ | </h5> | ||
+ | <p> | ||
+ | Keep it cool until it reaches <br> | ||
+ | around 40-50 °C | ||
+ | </p> | ||
+ | </div> | ||
+ | |||
+ | <div class="caption3"> | ||
+ | <h5> | ||
+ | <font color="white"> 23 </font> | ||
+ | </h5> | ||
+ | <p> | ||
+ | Add 200 μL of 1000x chloramphenicol <br> | ||
+ | and gently stir it. Be careful not <br> | ||
+ | to shake the bottle too long/hard so that | ||
+ | bubbles are created.<br> | ||
+ | </p> | ||
+ | </div> | ||
+ | |||
+ | <div class="caption4"> | ||
+ | <h5> | ||
+ | <font color="white"> 24 </font> | ||
+ | </h5> | ||
+ | <p> | ||
+ | Pour into empty petri dishes <br> | ||
+ | just enough to cover the surface<br> | ||
+ | (~20 mL per plate).<br> | ||
+ | In case that bubbles are in <br> | ||
+ | the plate, heat the plate surface <br> | ||
+ | carefully with a burner only <br> | ||
+ | until the bubbles are <br> | ||
+ | burst but the solution is heated. | ||
+ | </p> | ||
+ | </div> | ||
+ | |||
+ | <div class="caption4"> | ||
+ | <h5> | ||
+ | <font color="white"> 25 </font> | ||
+ | </h5> | ||
+ | <p> | ||
+ | Leave the plates at room <br> | ||
+ | temperature around one hour <br> | ||
+ | until it is solidified. | ||
+ | </p> | ||
+ | </div> | ||
+ | </font> | ||
+ | </div> | ||
+ | |||
+ | <div class="caption4"> | ||
+ | <h5> | ||
+ | <font color="white"> 26 </font> | ||
+ | </h5> | ||
+ | <p> | ||
+ | <font color="white"> | ||
+ | Solidified plates should be <br> | ||
+ | turned upside down for a <br> | ||
+ | few hours at room temperature,<br> | ||
+ | then stored at 4°C. | ||
+ | </font> | ||
+ | </p> | ||
+ | </div> | ||
+ | </font> | ||
</div> | </div> | ||
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</font> </h6> | </font> </h6> | ||
</section> | </section> | ||
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<script> | <script> |
Revision as of 12:25, 16 September 2015
Safety First!
Saving the world starts with saving ourselves. You cannot save the world unless you’re in good shape. We put safety on the top priority
in any lab works. We have been informed accurately of the lab safety rules as well as bio-safety regulations at the
initial stage of the project in order to understand hazards and safety procedures. Also we received safety training from our instructor
before commencing our lab work. Even though our project is included Bio-Safety Level 1, we know very well that
there are still many risks that may be escalated into serious disaster. Furthermore, even when handling those risks that may not pose serious
threats to the experienced scientists, we as young learners should be extra careful.
The rule of thumb is:
To The Top
We have conducted our experiments at Gachon University. Thus, we have always observed safety rules of the lab. We had Safety Education & Training Session
before starting our lab work. Also, when we conducted our experiments, Gachon University staff supervised our work.