Difference between revisions of "Team:Waterloo/Experiments"

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<li>Seeds of Arabidopsis thaliana</li>
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<li>Agrobacterium tumefaciens strains such as C58C1</li>
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<li>Luria-Bertani (LB) media (broth and agar plate)</li>
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<li>Induction medium: 10.5 g/L K2HPO4, 1 g/L (NH4)2SO4, 0.5 g/L NaCitrate, 1 g/L glucose, 1 g/L fructose, 4 g/L glycerol, 1 mM MgSO4, 10 mM 2-(N-morpholino) methanesulfonic acid (MES); adjust pH to 5.6, autoclave before use</li>
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<li>Infiltration medium: 10 mM MgSO4, 10 mM MES; adjust pH to 5.6, autoclave before use</li>
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<li>Rifampicin: dissolve in methanol to make 25 mg/mL stock solution and store in -20 C freezer</li>
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<li>Tetracycline: dissolve in 70% ethanol to make 12.5 mg/mL stock solution and store in -20 C freezer</li>
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<li>Kanamycin: dissolve in water to make 50 mg/mL stock solution and store in -20 C freezer</li>
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<li>Acetosyringone (3,5-dimethoxy-4-hydroxyacetophenone, Aldrich): dissolve in 196.2 mg acetosyringone in 1 mL dimethylsulfoxide to make 1 M stock solution and store in -20 C freezer</li>
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<li>Dexamethasone (DEX, USB, Cleveland, OH): dissolve 78.4 mg DEX in 1 mL ethanol to make 200 mM stock solution</li>
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<li>5-bromo-4-chloro-3-indolyl B-D-glucuronide (X-Glu, Sigma, St. Louis, MO): dissolve X-Glu in dimethylformamide to make 200 mM stock solution and store in -20 C freezer</li>
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<li>GUS staining solution: 50 mM sodium phosphate (pH 7.0), 1 mM EDTA, 2 mM X-Glu, 0.05% SDS, 0.1% sodium N-lauryl-sarcosine, 0.1% Triton-X-100</li>
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<li>Vacuum pump</li></ol>
 
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Revision as of 01:01, 19 September 2015

Experiments & Protocols

  1. Seeds of Arabidopsis thaliana
  2. Agrobacterium tumefaciens strains such as C58C1
  3. Luria-Bertani (LB) media (broth and agar plate)
  4. Induction medium: 10.5 g/L K2HPO4, 1 g/L (NH4)2SO4, 0.5 g/L NaCitrate, 1 g/L glucose, 1 g/L fructose, 4 g/L glycerol, 1 mM MgSO4, 10 mM 2-(N-morpholino) methanesulfonic acid (MES); adjust pH to 5.6, autoclave before use
  5. Infiltration medium: 10 mM MgSO4, 10 mM MES; adjust pH to 5.6, autoclave before use
  6. Rifampicin: dissolve in methanol to make 25 mg/mL stock solution and store in -20 C freezer
  7. Tetracycline: dissolve in 70% ethanol to make 12.5 mg/mL stock solution and store in -20 C freezer
  8. Kanamycin: dissolve in water to make 50 mg/mL stock solution and store in -20 C freezer
  9. Acetosyringone (3,5-dimethoxy-4-hydroxyacetophenone, Aldrich): dissolve in 196.2 mg acetosyringone in 1 mL dimethylsulfoxide to make 1 M stock solution and store in -20 C freezer
  10. Dexamethasone (DEX, USB, Cleveland, OH): dissolve 78.4 mg DEX in 1 mL ethanol to make 200 mM stock solution
  11. 5-bromo-4-chloro-3-indolyl B-D-glucuronide (X-Glu, Sigma, St. Louis, MO): dissolve X-Glu in dimethylformamide to make 200 mM stock solution and store in -20 C freezer
  12. GUS staining solution: 50 mM sodium phosphate (pH 7.0), 1 mM EDTA, 2 mM X-Glu, 0.05% SDS, 0.1% sodium N-lauryl-sarcosine, 0.1% Triton-X-100
  13. Vacuum pump
Text 2
Text 3

Describe the experiments, research and protocols you used in your iGEM project.

What should this page contain?
  • Protocols
  • Experiments
  • Documentation of the development of your project

Inspiration

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