Difference between revisions of "Team:ETH Zurich/Basic Part"
Line 29: | Line 29: | ||
<p></p> | <p></p> | ||
− | <p>In this section we present our basic parts. We expressed <a href="https://2015.igem.org/Team:ETH_Zurich/Results#Bacterial_sensor">human Annexin V</a> in <i>Escherichia coli</i> and then made a | + | <p>In this section we present our basic parts. We expressed <a href="https://2015.igem.org/Team:ETH_Zurich/Results#Bacterial_sensor">human Annexin V</a> in <i>Escherichia coli</i> and then made a fusion protein with <a href="https://2015.igem.org/Team:ETH_Zurich/Results#Bacterial_sensor">Annexin V and the Ice Nucleation Protein (INP)</a>. The former was created to allow our bacteria to serve as detectors of apoptosis by specifically binding phosphatidylserine. </p> |
<p>Our other part is LldR, a protein required to regulate the lldR promoter. The <a href="https://2015.igem.org/Team:ETH_Zurich/Results#Characterization_of_the_LldR_promoter">expression of LldR</a> was necessary to characterize the natural and our <a href="https://2015.igem.org/Team:ETH_Zurich/Part_Collection">designed lldR promoters</a>. </p> | <p>Our other part is LldR, a protein required to regulate the lldR promoter. The <a href="https://2015.igem.org/Team:ETH_Zurich/Results#Characterization_of_the_LldR_promoter">expression of LldR</a> was necessary to characterize the natural and our <a href="https://2015.igem.org/Team:ETH_Zurich/Part_Collection">designed lldR promoters</a>. </p> | ||
Revision as of 21:12, 16 September 2015
- Project
- Modeling
- Lab
- Human
Practices - Parts
- About Us
Basic Parts
In this section we present our basic parts. We expressed human Annexin V in Escherichia coli and then made a fusion protein with Annexin V and the Ice Nucleation Protein (INP). The former was created to allow our bacteria to serve as detectors of apoptosis by specifically binding phosphatidylserine.
Our other part is LldR, a protein required to regulate the lldR promoter. The expression of LldR was necessary to characterize the natural and our designed lldR promoters.
Part |
Content |
Registry number |
Annexin V | Promoter J23114-RBS B0034- Annexin V optimized for Escherichia coli | BBa_K1847000 |
INP-Annexin V | Promoter J23114-RBS B0034- INP-Annexin V fusion protein | BBa_K1847015 |
LldR | lldR | BBa_K1847001 |
LldP-LldR | lldP-lldR | BBa_K1847001 |