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− | Inflammable chemicals including acetone, oil, or gas must be kept in the place where ventilation is easy and access is infrequent.
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− | Importing and exporting of high-pressure gas container must be done using transportation equipment.
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− | When storing high-pressure gas containers, they must be safely and firmly fixed, isolated from flammable substances and inflammatory materials.
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− | Experiments using flammable, explosive, toxic, or volatile gases or vapors must be carried out in a hood.
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− | Reagents container or cabinets for storing reagents must be kept in cool, well-ventilated places without direct sunlight, and far away from fire and heat sources.
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− | Experimental drugs shall be stored in a way not to be shaken by outside shock, and so that its storage bottles do not fall
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− | Toxic materials shall be handled using protective films, or other safety tents to prevent damages from splatter, heating or explosion so as to ensure the safety of the experimenter.
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− | Containers for chemical wastes shall not be left in the hallway or on the stairs, and shall not be stored in the corners of the laboratory or other invisible places.
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− | When performing microbiological tests, gloves shall be worn, and after the experiment, the used glassware shall be
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− | Inflammable chemicals including acetone, oil, or gas must be kept in the place where ventilation is easy and access is infrequent.
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− | Importing and exporting of high-pressure gas container must be done using transportation equipment.
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− | When storing high-pressure gas containers, they must be safely and firmly fixed, isolated from flammable substances and inflammatory materials.
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− | Other significant Safety Considerations.
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− | 1. Fire Regulations.
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− | When fire breaks out, the following guidelines shall apply.
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− | <font color="white"> 1) </font>
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− | Save lives.
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− | <font color="white"> 2) </font>
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− | Call the fire brigade.
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− | Alert people in the area.
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− | Extinguish the fire if possible.
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− | <font color="white"> 5) </font>
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− | Close doors to the area.
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− | Evacuate.
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− | <font color="white"> 7) </font>
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− | Reassemble outside the building at the designated meeting point.
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We have conducted our experiments at Gachon University. Thus, we have always observed safety rules of the lab. We had Safety Education & Training Session
before starting our lab work. Also, when we conducted our experiments, Gachon University staff supervised our work.
Gachon University General Lab Safety Rule
1
200 mL LB prepared fresh,
non-autoclaved
3
Shake until all solids
are dissolved
4
Autoclave for 20 min within 2 hr
5
Keep it cool until it reaches
around 40-50 °C
6
Add 200 μL of 1000x chloramphenicol
and gently stir it. Be careful not
to shake the bottle too long/hard so that
bubbles are created.
7
Pour into empty petri dishes
just enough to cover the surface
(~20 mL per plate).
In case that bubbles are in
the plate, heat the plate surface
carefully with a burner only
until the bubbles are
burst but the solution is heated.
8
Leave the plates at room
temperature around one hour
until it is solidified.
9
Solidified plates should be
turned upside down for a
few hours at room temperature,
then stored at 4°C.