Difference between revisions of "Team:EPF Lausanne/Description"

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<h2>dCas9-ω</h2>
 
<h2>dCas9-ω</h2>
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<p>dCa9s-ω is our main tool to build our project in <i>E. coli</i>. This catalytically dead Cas9 fused to the RNA polymerase ω subunit can act as an activator or as an inhibitor when provided the right guide RNA.</p>
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<h2>PAM rich URS J23117 promoter</h2>
 
<h2>PAM rich URS J23117 promoter</h2>
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<p>This promoter is composed of the weak J23117 promoter, BBa_J23117, fused to a PAM rich upstream regulatory region (URS) that is needed for dCas9 to bind as dCas9 cannot bind without a PAM sequence.</p>
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<h2>PAM rich URS J23117Alt promoter</h2>
 
<h2>PAM rich URS J23117Alt promoter</h2>
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<p>This promoter was made on the same template as PAM rich URS J23117 promoter, but was mutated to obtain different sequences for sgRNA targeting. It is also fused to a PAM rich upstream regulatory region (URS) that is needed for dCas9 to bind, as dCas9 cannot bind without a PAM sequence.</p>
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Revision as of 21:10, 16 September 2015

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

BIOBRICK IMPROVEMENTS

dCas9-ω






BBa_K1723000

PAM rich URS J23117 promoter






BBa_K1723001

PAM rich URS J23117Alt promoter






BBa_K1723005
EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

NOT PROOFREAD