Difference between revisions of "Team:UCSC/Fermentation"

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There are three main types of cellulases: endocellulases, exocellulases, and -glucosidases; each play a vital role in cellulose degradation. Endocellulases cleave the hydrogen bonds between crystalline cellulose polymers, exposing the free -linked polysaccharide chains. Exocellulases then bind the ends of loose cellulose strands and cleave the -D-glucose bonds between every two or three glucose subunits producing either cellobiose or cellotriose.-glucosidase converts the cellobiose or  is vital because ionic liquids used in cellulose pretreatment assist degradation of cellulose, but not into monomers for Hvo to metabolize.  
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The fermentation team worked on a carbon neutral solution by engineering the fermentation pathway in HVO to go complete conversion of glucose to Butanol. The research was based off a pathway in Clostridium Acetobutycillum (see figure 1).
 
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Revision as of 01:29, 17 September 2015

Fermentation: Butanol


Background:

The fermentation team worked on a carbon neutral solution by engineering the fermentation pathway in HVO to go complete conversion of glucose to Butanol. The research was based off a pathway in Clostridium Acetobutycillum (see figure 1).

Aims:

We chose to transform Haloferax Volcanii with a B-glucosidase. Our pipeline was to collect data from different cellulases across various organisms, determine which are secreted, and decide which to try to codon optimize to transform into Hvo.

Future Goals:

Transform Hvo with our - glucosidase(WT) - pTA963C constructs. Then, test each for expression and enzymatic activity.

View our Notebook for our methodology that lead to our results.

Alonzo Lee
  • Biomolecular Engineering
    Cowell College (UCSC)
    4th Year
    Overseer of all projects 
    From: Sacramento, CA

"I started this project nervous and afraid about my ability to not only perform in a laboratory setting but as a captain as well. Yet now that have gone through iGEM I have become both an experienced researcher and a decisive leader who is able to communicate with all levels. This project has been one of the most fulfilling experiences of my life and I only hope that in the future more potential researchers are able to live what I have lived. With new experiences, knowledge, and friends in hand I look back at back at my iGEM experience only wishing to live it once more."

Nina Sardesh
  • Bioelectronics Engineering
    Electrical Engineering minor
    Stevenson College (UCSC)
    4th Year
    Grant & Social Media Supervisor
    From: San Francisco, CA 

Sanusha Bijj
  • Biomolecular Engineering
    Bioinformatics minor
    College Nine (UCSC)
    4th Year
    Grant, Social Media, &
    Website Team
    From: San Jose, CA

"Being apart of iGEM was more than just an experience. I was able to not only learn new lab techniques, but also learn how to fundraise, communicate, and think broader. My contribution to the team not only include lab work but publicizing our research through newspapers, writing grants, and writing thank-you letters. Two important qualities I learned from this experience were patience and communication. I can definitely say that this experience has prepped me to deal with the future research labs that I will encounter."

Jackson DeKloe
  • Molecular, Cell, &
    Developmental Biology
    Bioinformatics minor
    Cowell College (UCSC)
    5th Year
    From: Fairfield, CA

Vijay Jayant
  • Molecular, Cell, &
    Developmental Biology
    Bioinformatics minor
    College Ten (UCSC)
    5th Year
    Graphic Deisgn, Video, &
    Website Team
    From: Fremont, CA

Isabel Madau
  • Biochemistry
    University of Edinburgh
    2nd Year
    Grant Team
    From: Baltimore, MD

Derek Brekke
  • Biomolecular Engineering
    Bioinformatics minor
    College Nine (UCSC)
    5th Year
    From: Aptos, CA








Fermentation: Ethanol

Kassandra Colao
  • Biomolecular Engineering
    Bioinformatics minor
    Merrill College (UCSC)
    5th Year
    From: Napa, CA

Megana Kunda
  • Biomolecular Engineering and
    Molecular, Cell, &
    Developmental Biology
    Bioinformatics minor
    Collge 10 (UCSC)
    4th Year
    From: Milpitas, CA

Kendal Prokopakis
  • Biomolecular Engineering
    Bioinformatics minor
    Stevenson College (UCSC)
    4th Year
    From: Santa Clara, CA