Difference between revisions of "Team:SZU China/Notebook"

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         <p id="heading">AND GATE</p>
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         <p id="heading">Notebook</p>
 
         <p id="brief">Combine Biological Unnatural Amino Acid（UAA）Orthogonal
 
         <p id="brief">Combine Biological Unnatural Amino Acid（UAA）Orthogonal
 
             <br>genectical system with logic "AND" gate gene circuit
 
             <br>genectical system with logic "AND" gate gene circuit
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<time class="cbp_tmtime" datetime="2013-04-10 18:30"><span>4/10/13</span> <span>18:30</span></time>
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<time class="cbp_tmtime" datetime="2013-04-10 18:30"><span>4/10/13</span> <span>April 2nd - April 23rd</span></time>
 
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<p>Winter purslane...</p>
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<p>To discuss the decision of team project: CANCER SNIPER BLADDER</p>
 
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<time class="cbp_tmtime" datetime="2013-04-11T12:04"><span>4/11/13</span> <span>12:04</span></time>
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<time class="cbp_tmtime" datetime="2013-04-11T12:04"><span>4/11/13</span> <span>April 28th - June 20th</span></time>
 
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<h2>Greens radish arugula</h2>
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<p>Caulie dandelion maize...</p>
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<p>Finished the project system in shuttle vector</p>
 
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<time class="cbp_tmtime" datetime="2013-04-13 05:36"><span>4/13/13</span> <span>05:36</span></time>
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<time class="cbp_tmtime" datetime="2013-04-13 05:36"><span>4/13/13</span> <span>June 26th</span></time>
 
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<h2>Sprout garlic kohlrabi</h2>
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<p>Transfected the two plasmid system in 5637 and T24 bladder cancer cells,and got a good result in Rluc(with amber mutation). Moreover,we got the control experiments data in human bladder fiber epithelial normal cells(HFC).</p>
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<p>Parsnip lotus root...</p>
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<time class="cbp_tmtime" datetime="2013-04-15 13:15"><span>4/15/13</span> <span>13:15</span></time>
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<time class="cbp_tmtime" datetime="2013-04-15 13:15"><span>4/15/13</span> <span>June 30th</span></time>
 
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<h2>Watercress ricebean</h2>
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                <p>Improved the original BioBrick TERT (BBa_K1722001). </p>
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<p>Peanut gourd nori...</p>
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<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>4/16/13</span> <span>July 10th - July 25th</span></time>
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<p>In order to increase the expression of the system,we remoulded the system in three palsmid.</p>
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<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>4/16/13</span> <span>July 12th</span></time>
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<p>We greeted Tito Jankowski visiting SZU-iGEM,who is the world's first bio hackerspaces BioCurious founder.</p>
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<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>4/16/13</span> <span>July 18th </span></time>
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<p>Began to distribute questionnaires Online<a href:"http://www.sojump.com/jq/5569949.aspx">http://www.sojump.com/jq/5569949.aspx</a></p>
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<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>4/16/13</span> <span>July 28th</span></time>
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<p>Transfected the three plasmid system in the T24  and 5637 bladder cancer cells,but its result was not so good.Therefore,we continued to use the two plasmid system. </p>
 
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</li>
 
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<li>
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<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>4/16/13</span> <span>21:30</span></time>
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<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>4/16/13</span> <span>July 30th</span></time>
 
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<h2>Courgette daikon</h2>
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<p>In order to increase the expression of the system,we remoulded the system in three palsmid.</p>
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<p>Parsley amaranth tigernut...</p>
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Revision as of 02:00, 18 September 2015

Notebook

Combine Biological Unnatural Amino Acid（UAA）Orthogonal
genectical system with logic "AND" gate gene circuit
to detect bladder cancer cells precisely

  • To discuss the decision of team project: CANCER SNIPER BLADDER

  • Finished the project system in shuttle vector

  • Transfected the two plasmid system in 5637 and T24 bladder cancer cells,and got a good result in Rluc(with amber mutation). Moreover,we got the control experiments data in human bladder fiber epithelial normal cells(HFC).

  • Improved the original BioBrick TERT (BBa_K1722001).

  • In order to increase the expression of the system,we remoulded the system in three palsmid.

  • We greeted Tito Jankowski visiting SZU-iGEM,who is the world's first bio hackerspaces BioCurious founder.

  • Began to distribute questionnaires Onlinehttp://www.sojump.com/jq/5569949.aspx

  • Transfected the three plasmid system in the T24 and 5637 bladder cancer cells,but its result was not so good.Therefore,we continued to use the two plasmid system.

  • In order to increase the expression of the system,we remoulded the system in three palsmid.