Difference between revisions of "Team:EPF Lausanne/Part Collection"

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         <h3><i>S. cerevisiae</i></h3>
 
         <h3><i>S. cerevisiae</i></h3>
 
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<p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Aliquid, nulla.</p>
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<h2>Biobrick name</h2>
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<p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Aliquid, nulla.</p>
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Revision as of 17:09, 17 September 2015

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

OUR PART COLLECTION

E. coli

dCas9-ω

dCa9s-ω is our main tool to build our project in E. coli. This catalytically dead Cas9 fused to the RNA polymerase ω subunit can act as an activator or as an inhibitor when provided the right guide RNA.






BBa_K1723000

PAM rich URS J23117 promoter

This promoter is composed of the weak J23117 promoter, BBa_J23117, fused to a PAM rich upstream regulatory region (URS) that is needed for dCas9 to bind as dCas9 cannot bind without a PAM sequence.






BBa_K1723001

sgRNA Z0 expressing cassette

the sgRNA (single guide-RNA) Z0 inhibit PAM rich URS J23117 promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence Z0 makes this part unique, and the scaffold is a common part to all the sgRNAs and is recognized by dCas9-ω.






BBa_1723002

sgRNA Z4 expressing cassette

the sgRNA Z4 activates PAM rich URS J23117 promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence Z4 makes this part unique, and the scaffold is a common part to all the sgRNAs and is recognized by dCas9-ω.

This part is not RFC10. Removing bad restriction site would imply a lose of function.






BBa_1723003

sgRNA Z35 expressing cassette

the sgRNA Z35 inhibit PAM rich URS J23117 promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence Z35 makes this part unique, and the scaffold is a common part to all the sgRNAs and is recognized by dCas9-ω.






BBa_K1723004

PAM rich URS J23117Alt promoter

This promoter was made on the same template as PAM rich URS J23117 promoter, but was mutated to obtain different sequences for sgRNA targeting. It is also fused to a PAM rich upstream regulatory region (URS) that is needed for dCas9 to bind, as dCas9 cannot bind without a PAM sequence.






BBa_K1723005

sgRNA X0 expressing cassette

the sgRNA (single guide-RNA) X0 inhibit PAM rich URS J23117Alt promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence X0 makes this part unique, and the scaffold is a common part to all the sgRNAs and is recognized by dCas9-ω.






BBa_K1723006

sgRNA X4 expressing cassette

the sgRNA X4 activates PAM rich URS J23117Alt promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence X4 makes this part unique, and the scaffold is a common part to all the sgRNAs and is recognized by dCas9-ω.






BBa_K1723007

sgRNA X35 expressing cassette

the sgRNA X35 activates PAM rich URS J23117Alt promoter when in complex with dCas9-ω. This part is an expressing cassette under the constitutive promoter pBAD. The sequence X35 makes this part unique, and the scaffold is a common part to all the sgRNAs and is recognized by dCas9-ω.






BBa_K1723008

S. cerevisiae

Biobrick name

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Aliquid, nulla.






Reference

Biobrick name

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Aliquid, nulla.






Reference

Biobrick name

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Aliquid, nulla.






Reference

Biobrick name

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Aliquid, nulla.






Reference

Biobrick name

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Aliquid, nulla.






Reference

Biobrick name

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Aliquid, nulla.






Reference

Biobrick name

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Aliquid, nulla.






Reference

Biobrick name

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Aliquid, nulla.






Reference

Biobrick name

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Aliquid, nulla.






Reference

Biobrick name

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Aliquid, nulla.






Reference

Biobrick name

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Aliquid, nulla.






Reference

Biobrick name

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Aliquid, nulla.






Reference

Biobrick name

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Aliquid, nulla.






Reference
EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

NOT PROOFREAD