Difference between revisions of "Team:Technion HS Israel/Notebook"
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Revision as of 14:43, 17 September 2015
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Home
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Project
More about our project
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Documents
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Modelling
We take our math seriously
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Software
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Team
We are a bunch of nerdy nerds
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Practices
Contents
- summary
- preparation
- Antibiotics preparation
- Chemical transformation
- DNA Kit Plate
- Gel and PCR Extraction
- Gel Prep
- Glycerol stock
- LB SOB SOC BA
- Ligation
- manual NEB (gibson assembly)
- MixandGo
- PCR
- Phosphorylation and blunt ligation
- Presto Mini Plasmid Kit Protocol
- Restriction enzyme
- Transformation to 3300 LG using electroporation
- AHL inducer experiment protocol
Notebook
Here is a summery of our notebook.
| Week | Design | Cloning | Experiment | Finish new BioBrick |
|---|---|---|---|---|
| Jul 5th to Jul 9th (2015) | Primers : OGEM 1 and 2 to open j09855 plasmid . Gblock :RBS- Aiia with a Gibson overlabs. |
we got j09855 part from the distribution kit. | - | - |
| June 12th to July 16th (2015) | Primers : OGEM 3 and 4 to got the k1767001 plasmid without LuxR , after doing the PCR we will get a linear DNA so we put two enzymes in the overlaps of the primers to close the plasmids. | PCR to open the j09855 plasmid.Gibson to add Aiia . | - | BBa_K1767001 |
| Aug. 2nd to Aug. 6th (2015) | - | We got p0440 part from the distribution kit. We built BBa_K1767002 and BBa_K1767004 Constructs by restriction enzymes. PCR to get the k1767001 plasmid without LuxR . |
- | BBa_K1767002 BBa_K1767004 |
| Aug. 9th to Aug. 13th (2015) | Primers : OGEM 5 and 6 to check if we succeed in the PCR ( Those primers for colony PCR) | We got I13521 part from the distribution kit. We built BBa_K1767003 and BBa_K1767005 Constructs by restriction enzymes. Colony PCR to check if we have LuxR in the plasmid. | - | BBa_K1767003 BBa_K1767005 |
| Aug. 16th to Aug. 20th (2015) | - | - | AHL experiment #1 | - |
| Aug. 23rd to Aug. 27th (2015) | Primers : OGEM 7 and 8 to check if we have yfp in the plasmid ( for colony PCR ). | We got I13003 part from the distribution kit. | AHL experiment #2 | - |
| Aug. 30th to Sept. 9th (2015) | Primers: OGEM 9 and 10 to replaced the RFP with RFP ( with LVA ) | We built BBa_K1767009 and BBa_K1767010 Constructs by restriction enzymes.Colony PCR to check if we have YFP in the plasmid. | - | BBa_K1767009 BBa_K1767010 |
| Sept. 6th to Sept. 11th (2015) | - | - | AHL experiment # 3 | - |
preparation
- 1. LB medium
- 2. Miniprep
July 12th
July 13th
July 15th
July 20th
July 22th
July 29th
august 2th
august 3th
august 4th
august 5th
august 6th
august 9th
august 10th
august 11th
august 12th
august 13th
august 16th
august 17th
august 18th
august 19th
august 20th
august 23th
august 24th
august 25th
august 26th
august 27th
august 29th
august 30th
august 31th
september 1th
september 2th
september 3th
september 5th
september 6th
september 7th
