Difference between revisions of "Team:Technion HS Israel/Project/Results"
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<li><a href="#characterize of YFP"><span>characterize of YFP</span></a></li> | <li><a href="#characterize of YFP"><span>characterize of YFP</span></a></li> | ||
<li><a href="#Test our genetic circuit"><span>characterize of YFP</span></a></li> | <li><a href="#Test our genetic circuit"><span>characterize of YFP</span></a></li> | ||
| + | <a name="characterize of YFP"><h4>characterize of YFP</h4></a> | ||
| + | <p>In order to understand each module of our system, we started to test and characterize the first part of the genetic circuit. To do so, we built a BioBrick consisting of a constitutive promoter (BBa_I14032) driving expression of LuxR followed by a Lux promoter (BBa_R0062). The Lux promoter is inactive in absence of the AHL-LuxR complex and can be activated when AHL is present. Downstream of the pLux promoter we fused a gene encoding a florescent protein, in this case yellow fluorescent protein (YFP) (see Figure 1A). </p> | ||
</html> | </html> | ||
Revision as of 17:46, 17 September 2015
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characterize of YFP
In order to understand each module of our system, we started to test and characterize the first part of the genetic circuit. To do so, we built a BioBrick consisting of a constitutive promoter (BBa_I14032) driving expression of LuxR followed by a Lux promoter (BBa_R0062). The Lux promoter is inactive in absence of the AHL-LuxR complex and can be activated when AHL is present. Downstream of the pLux promoter we fused a gene encoding a florescent protein, in this case yellow fluorescent protein (YFP) (see Figure 1A).