Difference between revisions of "Team:Stockholm/Protocols"
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<h2>Protocols</h2> | <h2>Protocols</h2> | ||
− | <a href=" | + | <a href="https://static.igem.org/mediawiki/2015/f/fb/STHLM_WB_protocol.pdf" >Western blot </a> |
<br> | <br> | ||
<a href="#" >Membrane stripping </a> | <a href="#" >Membrane stripping </a> | ||
<br> | <br> | ||
− | <a href=" | + | <a href="https://static.igem.org/mediawiki/2015/7/7b/STHLM_Spheroplast_protocol.pdf" >Spheroplast </a> |
<br> | <br> | ||
<a href="#" >FACS </a> | <a href="#" >FACS </a> | ||
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<a href="https://static.igem.org/mediawiki/2015/8/86/STHLM_Affinity_separation_protocol.pdf" >Affinity separation (unfinished) </a> | <a href="https://static.igem.org/mediawiki/2015/8/86/STHLM_Affinity_separation_protocol.pdf" >Affinity separation (unfinished) </a> | ||
<br> | <br> | ||
− | <a href=" | + | <a href="https://static.igem.org/mediawiki/2015/1/17/STHLM_DotBlot_protocol.pdf" >Dot blot </a> |
<br> | <br> | ||
<a href="https://static.igem.org/mediawiki/2015/9/9d/STHLM_ELISA_protocol.pdf" >ELISA</a> | <a href="https://static.igem.org/mediawiki/2015/9/9d/STHLM_ELISA_protocol.pdf" >ELISA</a> | ||
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<a href="https://static.igem.org/mediawiki/2015/0/05/STHLM_Alternative_Osmolarity_protocol.pdf" >Alternative osmolarity</a> | <a href="https://static.igem.org/mediawiki/2015/0/05/STHLM_Alternative_Osmolarity_protocol.pdf" >Alternative osmolarity</a> | ||
<br> | <br> | ||
− | <a href=" | + | <a href="https://static.igem.org/mediawiki/2015/5/59/STHLM_Overlap_PCR_protocol.pdf" >Overlap PCR </a> |
+ | <br> | ||
+ | <a href="https://static.igem.org/mediawiki/2015/c/cc/STHLM_Transformation_protocol.pdf" >Transformation </a> | ||
+ | <br> | ||
+ | <a href="#" > </a> | ||
<br> | <br> | ||
<a href="https://static.igem.org/mediawiki/2015/8/84/STHLM_Colony_PCR_protocol.pdf" >Colony PCR</a> | <a href="https://static.igem.org/mediawiki/2015/8/84/STHLM_Colony_PCR_protocol.pdf" >Colony PCR</a> |
Revision as of 17:06, 17 September 2015
Protocols
Western blotMembrane stripping
Spheroplast
FACS
Affinity separation (unfinished)
Dot blot
ELISA
Alternative osmolarity
Overlap PCR
Transformation
Colony PCR
Osmolarity
Site-directed mutagenesis
Adapted osmolarity
AHL osmolarity dependence
3A assembly
Chemically competent cells
Overnight cultures and restriction analysis
Traditional cloning (Restriction cloning)