Difference between revisions of "Team:Bielefeld-CeBiTec/InterlabStudy"
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Revision as of 20:17, 17 September 2015
Measurement Interlab Study
Here you find our results of the Interlab Study.
Chassis and Devices
As chassis we used Escherichia coli KRX. The required devices were created by Standard BioBrick Assembly (Suffix Insertion). For the measurement, the following devices and controls were used:
- Device 1: GFP Generator (BBa_I13504) under control of the Anderson promoter J23101 (BBa_K823005)
- Device 2: GFP Generator (BBa_I13504) under control of the Anderson promoter J23106 (BBa_K823008)
- Device 3: GFP Generator (BBa_I13504) under control of the Anderson promoter J23117 (BBa_K823013)
- Positive control (PC): GFP expression device (BBa_I20270)
- Negative control 1 (NC 1): pTetR (BBa_R0040)
- Negative control 2 (NC 2): E. coli KRX with no plasmid
The final devices were validated by DNA Sequencing and Restriction Mapping. You can find the protocol for growing cells for the measurement here: 2015 Interlab Protocol.
Results
