Difference between revisions of "Team:Bielefeld-CeBiTec/Notebook/Miscellaneous"
| Line 155: | Line 155: | ||
<div id="collapseNine" class="panel-collapse collapse"> | <div id="collapseNine" class="panel-collapse collapse"> | ||
<div class="panel-body"> | <div class="panel-body"> | ||
| − | <p>...</p> | + | <p> <ul> <li> fluorescence imaging</li> |
| + | <ul> <li> we took lysate with sfGFP, lysis buffer and purified GFP </li> | ||
| + | <li> We got a filter from light engineering from the women cultural center Bielefeld e.V. ("Frauenkulturzentrum Bielefeld e.V.") and put it in front of the smartphone. </li> | ||
| + | <li> the picture showed great evidence for possible fluorescence imaging with the right filters </li> </ul> </ul> | ||
| + | </p> | ||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 00:04, 18 September 2015
Miscellaneous
- fluorescence imaging
- we took lysate with sfGFP, lysis buffer and purified GFP
- We got a filter from light engineering from the women cultural center Bielefeld e.V. ("Frauenkulturzentrum Bielefeld e.V.") and put it in front of the smartphone.
- the picture showed great evidence for possible fluorescence imaging with the right filters
This Week we started with the iGEM 2015 Measurement Interlab Study:
- Heat shock transformation of BioBricks from the distribution:
- BBa_K823005 (Anderson promoter J23101)
- BBa_K823008 (Anderson promoter J23106)
- BBa_K823013 (Anderson promoter J23117)
- BBa_I13504 (GFP)
iGEM 2015 Measurement Interlab Study:
- Colonies from the BioBrick Transformation were used to inoculate over night cultures
- 5 ml LB-Medium + Cm (20 µg/ml), 37°C
- Mini-preps of KRX(BBa_K823005), KRX(BBa_K823008) and KRX(BBa_K823013)
- Plasmids stored at -20°C
- Second heat shock transformation of chem. competent KRX with BBa_I13504
- Colonies were used to inoculate over night cultures (5 ml LB + Cm20, 37°C)
iGEM 2015 Measurement Interlab Study:
- Mini-prep of KRX(BBa_I13504)
- Plasmid stored at -20°C