Difference between revisions of "Team:CU Boulder/Parts/team"
| Line 49: | Line 49: | ||
<div id="section_01"> | <div id="section_01"> | ||
<div class="left"> | <div class="left"> | ||
| − | <b>RFP under arabinose inducible pBad</b> | + | <p><b>RFP under arabinose inducible pBad</b></p> |
<p>This part has a Red Fluorescent Protein (RFP) downstream of the pBad promoter. Expression is stimulated by L-arabinose. We used this part to test the leakiness of this promoter.</p> | <p>This part has a Red Fluorescent Protein (RFP) downstream of the pBad promoter. Expression is stimulated by L-arabinose. We used this part to test the leakiness of this promoter.</p> | ||
</div> | </div> | ||
| Line 57: | Line 57: | ||
<h2>BBa_K1718002:</h2> | <h2>BBa_K1718002:</h2> | ||
<div id="section_02"> | <div id="section_02"> | ||
| − | <b>Genetic Switch for Bxb1 invertase</b> | + | <p><b>Genetic Switch for Bxb1 invertase</b></p> |
<p>The Bxb1 invertase will invert the segment of DNA between the two recognition sites. In this case, Bxb1 will invert a terminator allowing for expression of a strong Anderson promoter.</p> | <p>The Bxb1 invertase will invert the segment of DNA between the two recognition sites. In this case, Bxb1 will invert a terminator allowing for expression of a strong Anderson promoter.</p> | ||
| Line 64: | Line 64: | ||
<h2>BBa_K1718003:</h3> | <h2>BBa_K1718003:</h3> | ||
<div id="section_03"> | <div id="section_03"> | ||
| − | <b>RFP under the LuxPr promoter</b> | + | <p><b>RFP under the LuxPr promoter</b></p> |
<p>In the presence of AHL, the LuxR transcription factor will bind AHL and activate the LuxPr promoter. RFP is downstream and will be expressed.</p> | <p>In the presence of AHL, the LuxR transcription factor will bind AHL and activate the LuxPr promoter. RFP is downstream and will be expressed.</p> | ||
| Line 71: | Line 71: | ||
<h2>BBa_K1718004:</h2> | <h2>BBa_K1718004:</h2> | ||
<div id="section_04"> | <div id="section_04"> | ||
| + | <p><b>LuxI and GFP</b></p> | ||
| + | <p>LuxI leads to the production of AHL which, when in the presence of AHL, activates the LuxPr promoter. Downstream of the LuxI coding region is GFP, a reporter</p> | ||
</div> | </div> | ||
| Line 76: | Line 78: | ||
<h2>BBa_K1718005:</h2> | <h2>BBa_K1718005:</h2> | ||
<div id="section_05"> | <div id="section_05"> | ||
| + | <p><b>Genetic switch for Bxb1 with LuxI and GFP</b></p> | ||
| + | <p>This part consists of the genetic switch described in BBa_K1718002 and the LuxI and GFP coding regions described in BBa_K1718004</p> | ||
</div> | </div> | ||
Revision as of 02:05, 18 September 2015
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