Difference between revisions of "Team:SZU China/Notebook"

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<p>In order to increase the expression of the system,we remoulded the system in three palsmid.</p>
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<p>Propaganda synthetic biology and iGEM to all students at the Cultural Festival of the Collage of Life Science.</p>
 
</div>
 
</div>
 
</li>
 
</li>
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<li>
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<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>4/16/13</span> <span>August 7th - August 15th</span></time>
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<div class="cbp_tmlabel">
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<p>Finished some BioBricks:shTERT+GFP Composite(BBa_K1722009), TERT part(BBa_K1722002), shTERT part(BBa_K1722001),hUPII part(BBa_K1722000) and Rlu part(BBa_K1722005).</p>
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</div>
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</li>
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<li>
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<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>4/16/13</span> <span>August 16th - August 25th</span></time>
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<div class="cbp_tmicon cbp_tmicon-earth"></div>
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<div class="cbp_tmlabel">
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<p>Added GFP(with amber mutation) to the two plasmid system, and transfected the system in 5637 and T24 bladder cancer cells.</p>
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</div>
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</li>
 +
<li>
 +
<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>4/16/13</span> <span>August 18th - August 26th</span></time>
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<div class="cbp_tmicon cbp_tmicon-earth"></div>
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<div class="cbp_tmlabel">
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<p>Finished some BioBricks:SV40(en) part(BBa_K1722006), SV40+Rlu Composite(BBa_K1722013), tRNA part(BBa_K1722004), SV40(en)+Rlu Composite(BBa_K1722012)</p>
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</div>
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</li>
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<li>
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<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>4/16/13</span> <span>August 19th </span></time>
 +
<div class="cbp_tmicon cbp_tmicon-earth"></div>
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<div class="cbp_tmlabel">
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<p>LZU-iGEM and SZU-iGEM made a whole day of cooperative communication.
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</p>
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</div>
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</li>
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<li>
 +
<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>4/16/13</span> <span>August 23th</span></time>
 +
<div class="cbp_tmicon cbp_tmicon-earth"></div>
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<div class="cbp_tmlabel">
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<p>The GFP gene transfection test was successful, and it proved that THE BLADDER CANCER SNIPER SYSTERM was functional.</p>
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</div>
 +
</li>
 +
<li>
 +
<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>4/16/13</span> <span>August 26th - September 10th</span></time>
 +
<div class="cbp_tmicon cbp_tmicon-earth"></div>
 +
<div class="cbp_tmlabel">
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<p>Finished the rest of BioBricks:ShTERT+tRNA Composite(BBa_K1722011),hTERT+tRNA Composite（BBa_K1722010），AckRS part(BBa_K1722003),hUPll+AckRS Composite(BBa_K1722007)
 +
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</p>
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</div>
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</li>
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<li>
 +
<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>4/16/13</span> <span>September 2th - September 13th</span></time>
 +
<div class="cbp_tmicon cbp_tmicon-earth"></div>
 +
<div class="cbp_tmlabel">
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<p>Finished the rest of BioBricks:hUPll+AckRS Composite(BBa_K1722007),AckRS part(BBa_K1722003),ShTERT+tRNA Composite(BBa_K1722011),hTERT+tRNA Composite(BBa_K1722010) </p>
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</div>
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</li>
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<li>
 +
<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>4/16/13</span> <span>September 3th - September 15th</span></time>
 +
<div class="cbp_tmicon cbp_tmicon-earth"></div>
 +
<div class="cbp_tmlabel">
 +
<p>Finished collecting BioBricks data, and edited in the wiki.<a href=" https://2015.igem.org/Team:SZU_China/Parts">https://2015.igem.org/Team:SZU_China/Parts</a></p>
 +
</div>
 +
</li>
 +
<li>
 +
<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>4/16/13</span> <span>September 5th </span></time>
 +
<div class="cbp_tmicon cbp_tmicon-earth"></div>
 +
<div class="cbp_tmlabel">
 +
<p>we visited the Shenzhen Cancer Rehabilitation Association,and made a thorough understanding about specific situation of cancer patients.</p>
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</div>
 +
</li>
 +
<li>
 +
<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>4/16/13</span> <span>September 8th - September 15th</span></time>
 +
<div class="cbp_tmicon cbp_tmicon-earth"></div>
 +
<div class="cbp_tmlabel">
 +
<p>In order to prove the system's function and specificity, we made second GFP transfection test.The result indicated the system has a good function and specificity.
 +
 +
</p>
 +
</div>
 +
</li>
 +
<li>
 +
<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>4/16/13</span> <span>September 11th  </span></time>
 +
<div class="cbp_tmicon cbp_tmicon-earth"></div>
 +
<div class="cbp_tmlabel">
 +
<p>According to our experience, we convened cancer patients,and made a cancer patients communication,Which purpose is to give patients confidence and improve the difficult situation of group's treatment in china.
 +
 +
</p>
 +
</div>
 +
</li>
 +
 
</ul>
 
</ul>
  

Revision as of 02:10, 18 September 2015

Notebook

Combine Biological Unnatural Amino Acid（UAA）Orthogonal
genectical system with logic "AND" gate gene circuit
to detect bladder cancer cells precisely

  • To discuss the decision of team project: CANCER SNIPER BLADDER

  • Finished the project system in shuttle vector

  • Transfected the two plasmid system in 5637 and T24 bladder cancer cells,and got a good result in Rluc(with amber mutation). Moreover,we got the control experiments data in human bladder fiber epithelial normal cells(HFC).

  • Improved the original BioBrick TERT (BBa_K1722001).

  • In order to increase the expression of the system,we remoulded the system in three palsmid.

  • We greeted Tito Jankowski visiting SZU-iGEM,who is the world's first bio hackerspaces BioCurious founder.

  • Began to distribute questionnaires Onlinehttp://www.sojump.com/jq/5569949.aspx

  • Transfected the three plasmid system in the T24 and 5637 bladder cancer cells,but its result was not so good.Therefore,we continued to use the two plasmid system.

  • Propaganda synthetic biology and iGEM to all students at the Cultural Festival of the Collage of Life Science.

  • Finished some BioBricks:shTERT+GFP Composite(BBa_K1722009), TERT part(BBa_K1722002), shTERT part(BBa_K1722001),hUPII part(BBa_K1722000) and Rlu part(BBa_K1722005).

  • Added GFP(with amber mutation) to the two plasmid system, and transfected the system in 5637 and T24 bladder cancer cells.

  • Finished some BioBricks:SV40(en) part(BBa_K1722006), SV40+Rlu Composite(BBa_K1722013), tRNA part(BBa_K1722004), SV40(en)+Rlu Composite(BBa_K1722012)

  • LZU-iGEM and SZU-iGEM made a whole day of cooperative communication.

  • The GFP gene transfection test was successful, and it proved that THE BLADDER CANCER SNIPER SYSTERM was functional.

  • Finished the rest of BioBricks:ShTERT+tRNA Composite(BBa_K1722011),hTERT+tRNA Composite（BBa_K1722010），AckRS part(BBa_K1722003),hUPll+AckRS Composite(BBa_K1722007)

  • Finished the rest of BioBricks:hUPll+AckRS Composite(BBa_K1722007),AckRS part(BBa_K1722003),ShTERT+tRNA Composite(BBa_K1722011),hTERT+tRNA Composite(BBa_K1722010)

  • Finished collecting BioBricks data, and edited in the wiki.https://2015.igem.org/Team:SZU_China/Parts

  • we visited the Shenzhen Cancer Rehabilitation Association,and made a thorough understanding about specific situation of cancer patients.

  • In order to prove the system's function and specificity, we made second GFP transfection test.The result indicated the system has a good function and specificity.

  • According to our experience, we convened cancer patients,and made a cancer patients communication,Which purpose is to give patients confidence and improve the difficult situation of group's treatment in china.