Difference between revisions of "Team:Gifu/team-profile"
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− | <table width="800" style="border: currentColor; border-image: none; margin-right: auto; margin-left: auto;" border="0" cellspacing="0" cellpadding="0"><tbody><tr><td width="30%" nowrap="" style="text-align: left; padding-right: 2em;"><b>Title and Abstract</b><a style="color: rgb(102, 102, 255); padding-left: 15px; font-size: 90%;" href="https://2014.igem.org/Jamboree/Title_and_Abstract"><i>More...</i></a><td align="center"> | + | <table width="800" style="border: currentColor; border-image: none; margin-right: auto; margin-left: auto;" border="0" cellspacing="0" cellpadding="0"><tbody><tr><td width="30%" nowrap="" style="text-align: left; padding-right: 2em;"><b>Title and Abstract</b><a style="color: rgb(102, 102, 255); padding-left: 15px; font-size: 90%;" href="https://2014.igem.org/Jamboree/Title_and_Abstract"><i>More...</i></a><td align="center"><A style='text-decoration: none; color: blue;' |
HREF='abstract_pdf_file.cgi?id=1859' ><i>Download a .pdf version</i></a><td width="30%" align="right"></tr></tbody></table><table width="800" id="table_abstract" style="padding: 3px 2px 0px 15px; border: 1px solid gray; border-image: none; line-height: 150%; margin-right: auto; margin-left: auto; background-color: rgb(220, 220, 220);" border="0" cellspacing="0" cellpadding="0"> | HREF='abstract_pdf_file.cgi?id=1859' ><i>Download a .pdf version</i></a><td width="30%" align="right"></tr></tbody></table><table width="800" id="table_abstract" style="padding: 3px 2px 0px 15px; border: 1px solid gray; border-image: none; line-height: 150%; margin-right: auto; margin-left: auto; background-color: rgb(220, 220, 220);" border="0" cellspacing="0" cellpadding="0"> | ||
− | <tbody><tr><td style="padding: 5px 0px; width: 750px; font-size: 10pt; font-weight: bold;" colspan="9">Circular mRNA | + | <tbody><tr><td style="padding: 5px 0px; width: 750px; font-size: 10pt; font-weight: bold;" colspan="9">Circular mRNA ver.2.0<br> |
− | <tr><td style="padding: 5px 0px 5px 20px; width: 750px; vertical-align: top;" colspan="9"> | + | <tr><td style="padding: 5px 0px 5px 20px; width: 750px; vertical-align: top;" colspan="9">  In last year, we succeeded in designing the sequence which synthesizes circular mRNA and long chain protein in <i>Escherichia coli</i>.<br> |
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+ |  In this year, we had 2 purposes in our study. One was an efficiency of the circularization. The efficiency was lower in our previous study. This was why the splicing was hard to happen because two sequences to act as ribozyme for splicing were far each other. So we incorporated <b>complementary sequences</b> around the ribozyme regions. We thought that the treatment brought two regions close and the efficiency of the circularization improved.<br> | ||
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+ | The other was to synthesize useful proteins. In our previous study, synthesized long-chain proteins lose their function because the folding of the proteins was broken. So we incorporated <b>linker sequences</b> into circular mRNA to synthesize the functional long-chain protein.<br></tr></tbody></table><br> | ||
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Revision as of 03:34, 18 September 2015
TEAM PROFILE
Team Name: | Gifu | ||||||||
Primary Contact (PI): | Hitoshi IWAHASHI | ||||||||
Schools: | Gifu University Yanagido, Gifu, Japan | ||||||||
Division: | iGEM | Application Date: 2015-04-27 | |||||||
Section: | Undergraduate | ||||||||
Region: | Asia | Acceptance Date: 2015-05-02 08:32:07 | |||||||
Description: |
Team Status |
Welcome to iGEM 2015 ! Your iGEM 2015 team application was accepted by iGEM Headquarters on 2015-05-02 08:32:07 and your team registration fee has been received. |
DNA Kit of Parts shipping information has been entered, thanks. View shipping information |
Resource description has been submitted, thanks. View resource description |
Your registration fee has been received. Thank you.
More... |
Safety |
Check In FormAbout Our Lab FormAbout Our Project FormFinal Safety Form |
TrackMore... |
Assigned Track: New Application |
Title and AbstractMore... | Download a .pdf version |
Circular mRNA ver.2.0 | ||||||||
In last year, we succeeded in designing the sequence which synthesizes circular mRNA and long chain protein in Escherichia coli. In this year, we had 2 purposes in our study. One was an efficiency of the circularization. The efficiency was lower in our previous study. This was why the splicing was hard to happen because two sequences to act as ribozyme for splicing were far each other. So we incorporated complementary sequences around the ribozyme regions. We thought that the treatment brought two regions close and the efficiency of the circularization improved. The other was to synthesize useful proteins. In our previous study, synthesized long-chain proteins lose their function because the folding of the proteins was broken. So we incorporated linker sequences into circular mRNA to synthesize the functional long-chain protein. |
Team Roster |
Instructors | |||
h1884 | Hitoshi IWAHASHI | ||
isatoshi | Satoshi IWAMOTO | ||
aebihara2014 | Akio Ebihara |
Student Members | |||
momochi | Momoko YAMADA | ||
Kairi | Kairi ISHIMARU | ||
nomuken | Nomura Kenta | ||
kozakai | Kozakai Tomoya | ||
fumiki | Fumiki Izumi | ||
liyu | son liyu | ||
FreshLemon | Haruka Maruyama | ||
takema | Hasegawa Takema | ||
ybanno | Yusuke Banno | ||
Mori | Akihiro Moriyama | ||
fukufuku | Wataru Fukuda | ||
SachiA | Sachi Asano |
This range of part numbers has been assigned to your team for use during the summer: BBa_K1859000 to BBa_K1859999 |