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Revision as of 13:44, 18 September 2015
Notebook
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To discuss the decision of team project: CANCER SNIPER BLADDER
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Finished the project system in shuttle vector
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Transfected the two plasmid system in 5637 and T24 bladder cancer cells,and got a good result in Rluc(with amber mutation). Moreover,we got the control experiments data in human bladder fiber epithelial normal cells(HFC).
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Improved the original BioBrick TERT (BBa_K1722001).
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In order to increase the expression of the system,we remoulded the system in three palsmid.
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We greeted Tito Jankowski visiting SZU-iGEM,who is the world's first bio hackerspaces BioCurious founder.
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Began to distribute questionnaires Onlinehttp://www.sojump.com/jq/5569949.aspx
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Transfected the three plasmid system in the T24 and 5637 bladder cancer cells,but its result was not so good.Therefore,we continued to use the two plasmid system.
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Propaganda synthetic biology and iGEM to all students at the Cultural Festival of the Collage of Life Science.
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Finished some BioBricks:shTERT+GFP Composite(BBa_K1722009), TERT part(BBa_K1722002), shTERT part(BBa_K1722001),hUPII part(BBa_K1722000) and Rlu part(BBa_K1722005).
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Added GFP(with amber mutation) to the two plasmid system, and transfected the system in 5637 and T24 bladder cancer cells.
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Finished some BioBricks:SV40(en) part(BBa_K1722006), SV40+Rlu Composite(BBa_K1722013), tRNA part(BBa_K1722004), SV40(en)+Rlu Composite(BBa_K1722012)
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LZU-iGEM and SZU-iGEM made a whole day of cooperative communication.
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The GFP gene transfection test was successful, and it proved that THE BLADDER CANCER SNIPER SYSTERM was functional.
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Finished the rest of BioBricks:ShTERT+tRNA Composite(BBa_K1722011),hTERT+tRNA Composite(BBa_K1722010),AckRS part(BBa_K1722003),hUPll+AckRS Composite(BBa_K1722007)
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Finished the rest of BioBricks:hUPll+AckRS Composite(BBa_K1722007),AckRS part(BBa_K1722003),ShTERT+tRNA Composite(BBa_K1722011),hTERT+tRNA Composite(BBa_K1722010)
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Finished collecting BioBricks data, and edited in the wiki.https://2015.igem.org/Team:SZU_China/Parts
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we visited the Shenzhen Cancer Rehabilitation Association,and made a thorough understanding about specific situation of cancer patients.
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In order to prove the system's function and specificity, we made second GFP transfection test.The result indicated the system has a good function and specificity.
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According to our experience, we convened cancer patients,and made a cancer patients communication,Which purpose is to give patients confidence and improve the difficult situation of group's treatment in china.