Difference between revisions of "Team:UC San Diego/Notebook"

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<h2>September 16</h2>
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<p>Library presentation at La Jolla Riford Library.</p>
 
<p>Library presentation at La Jolla Riford Library.</p>
 
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<p>High school meet up.</p>
 
<p>High school meet up.</p>
 
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<h2>August 6th</h2>
 
<p>Southern California iGEM Meetup</p>
 
<p>Southern California iGEM Meetup</p>
 
<p><a href="https://static.igem.org/mediawiki/2015/9/99/UC_San_Diego_SoCal.jpg" data-lightbox="socal" data-title="Southern California Meet Up caption"><img width="50" height="50" src="https://static.igem.org/mediawiki/2015/9/99/UC_San_Diego_SoCal.jpg"></a></p>
 
<p><a href="https://static.igem.org/mediawiki/2015/9/99/UC_San_Diego_SoCal.jpg" data-lightbox="socal" data-title="Southern California Meet Up caption"><img width="50" height="50" src="https://static.igem.org/mediawiki/2015/9/99/UC_San_Diego_SoCal.jpg"></a></p>

Revision as of 09:13, 18 September 2015

TIMELINE

September 24-28

Giant Jamboree!

Giant Jamboree!

September 16th

Library presentation at La Jolla Riford Library.

Read more! WEEK 14

Modeling

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WEEK 14

Wet Lab

+Final preparations before the wiki freeze.

WEEK 14

Modeling

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WEEK 13

Wet Lab

+ Mapped out errors in the parts that we assembled
+ Mutagenesis to fix recurring errors in C sequence

WEEK 13

September 4th

High school meet up.

Read more! WEEK 12

Modeling

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WEEK 12

Wet Lab

+ Attempted Gibson Assembly with CDE with AB
+ Designed sequence primers
+ Miniprepped full size clones

WEEK 12

Modeling

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WEEK 11

Wet Lab

+ Attempted full gibson assembly of AB fragment
+ Miniprepped CDE fragments

WEEK 11

Modeling

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WEEK 10

Wet Lab

+ Selected error free clones for CDE fragments from sequencing data
+ Designed primers for PacBio Sequencing
+ Transformed error free clones of CDE

WEEK 10

Modeling

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WEEK 9

Wet Lab

+ Assembled using Gibson Assembly and sequenced CDE fragments
+ PCR and transformed fragments

WEEK 9

August 6th

Southern California iGEM Meetup

Read more! Week 8

Modeling

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WEEK 8

Wet Lab

+ Received our DNA fragments from SGI.
+ Attempted to PCR and transform our fragments.

WEEK 8

Modeling

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WEEK 7

Wet Lab

+ Assembled Interlab Devices. Resuspended and measured them successfully.
+ Prepared YPD plates.

WEEK 7

Modeling

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WEEK 6

Wet Lab

+ Continued interlab study with miniprep, restriction digests, ligation, gel electrophoresis and gel purification.

WEEK 6

Modeling

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WEEK 5

Wet Lab

+ Started interlab study with transformation.

WEEK 5

Modeling

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WEEK 4

Wet Lab

+ Sent our genes to SGI and ordered lab supplies. Almost ready to go!

WEEK 4

Modeling

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WEEK 3

Wet Lab

+ Added frp gene sequence from Vibrio Harveyi to our plasmid to stabilize the production of luciferase.
+ Optimized our plasmid sequences.

WEEK 3

Modeling

Read papers on drive
Read about metabolic control analysis
Compiled notes on bioluminescent system
Literature research:understanding bioluminescent mechanism
> aldehyde synthesis
> FMNH2 production
> light production
Familiarized with COBRA module

Primer to Genome-Scale Modeled and COBRA Tutorial (Jahir)

Continued literature search for key mechanisms regarding our auto-induced bioluminescent reaction in a yeast model
>auto-induced therefore no coupling between cells (removed LuxI and LuxR genes)

WEEK 2

Wet Lab

+ Created a preliminary design for the plasmids using ApE.
+ Improved them over the week by adding tags, removed illegal restriction sites, and changing repetitive sequences.

WEEK 2

Modeling

Developed Primer to MATLAB Programming
> lecture material from MIT course and UCSD course
> reading material from UCSD course
> study and practice material MIT course and UCSD course

WEEK 1

Wet Lab

+ Found genes coding for fatty acid reductase complex that have been validated in an in vitro synthesis paper.
+ Planned how to assemble our plasmids and determined nucleotide sequences for lux A-E of Photobacterium Phosphoreum.
+ Compared the amino acid sequences to those of other organisms on BLAST to check for significant discrepancies in our sequence.

WEEK 1