Difference between revisions of "Team:Bielefeld-CeBiTec/Protocols"
| Line 36: | Line 36: | ||
<div class="panel-heading"> | <div class="panel-heading"> | ||
<h4 class="panel-title"> | <h4 class="panel-title"> | ||
| − | <a data-toggle="collapse" href="# | + | <a data-toggle="collapse" href="#ColonyPCR">Colony PCR</a> |
</h4> | </h4> | ||
</div> | </div> | ||
| − | <div id=" | + | <div id="ColonyPCR" class="panel-collapse collapse"> |
<div class="panel-body"> | <div class="panel-body"> | ||
<p>...</p> | <p>...</p> | ||
Revision as of 21:15, 9 August 2015
Protocols
...
...
...
...
- Thaw 50 µl electrocompetent E. coli cells on ice, dilute with icecold 50 µl glycerine (10%) if necessary
- Add 0.5-5 µl plasmid to 50 µl electrocompetent cells
- Store cells on ice for 1 minute
- Electroporate at U = 2.5 kV, C = 25 µF, R = 400 Ω
- Transfer transformation reaction to 450 µl SOC-Medium and incubate 1 h at 37 °C
- Plate on selective LB-Medium
- Incubate over night at 37 °C