Difference between revisions of "Team:Technion HS Israel/Notebook"
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− | <a name=" | + | <a name="August"><h3>August</h3></a> |
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+ | <p> & nbsp;<a href="#august 2th"><span>August 2th</span></a> | ||
+ | & nbsp;<a href="#august 3th"><span>August 3th</span></a> | ||
+ | & nbsp;<a href="#august 4th"><span>August 4th</span></a> | ||
+ | & nbsp;<a href="#august 5th"><span>August 5th</span></a> | ||
+ | & nbsp;<a href="#august 6th"><span>August 6th</span></a> | ||
+ | & nbsp;<a href="#august 9th"><span>August 9th</span></a> | ||
+ | & nbsp;<a href="#august 10th"><span>August 10th</span></a> | ||
+ | & nbsp;<a href="#august 11th"><span>August 11th</span></a> | ||
+ | & nbsp;<a href="#august 12th"><span>August 12th</span></a> | ||
+ | & nbsp;<a href="#august 13th"><span>August 13th</span></a> | ||
+ | & nbsp;<a href="#august 16th"><span>August 16th</span></a> | ||
+ | & nbsp;<a href="#august 17th"><span>August 17th</span></a> | ||
+ | & nbsp;<a href="#august 18th"><span>August 18th</span></a> | ||
+ | & nbsp;<a href="#august 19th"><span>August 19th</span></a> | ||
+ | & nbsp;<a href="#august 20th"><span>August 20th</span></a> | ||
+ | & nbsp;<a href="#august 23th"><span>August 23th</span></a> | ||
+ | & nbsp;<a href="#august 24th"><span>August 24th</span></a> | ||
+ | & nbsp;<a href="#august 25th"><span>August 25th</span></a> | ||
+ | & nbsp;<a href="#august 26th"><span>August 26th</span></a> | ||
+ | & nbsp;<a href="#august 27th"><span>August 27th</span></a> | ||
+ | & nbsp;<a href="#august 29th"><span>August 29th</span></a> | ||
+ | & nbsp;<a href="#august 30th"><span>August 30th</span></a> | ||
+ | & nbsp;<a href="#august 31th"><span>August 31th</span></a> </p> | ||
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Revision as of 22:49, 18 September 2015
Notebook
Here is a summery of our notebook.
Week | Design | Cloning | Experiment | Finish new BioBrick |
---|---|---|---|---|
Jul 5th to Jul 9th (2015) | Primers : OGEM 1 and 2 to open j09855 plasmid . Gblock :RBS- Aiia with a Gibson overlabs. |
we got j09855 part from the distribution kit. | - | - |
June 12th to July 16th (2015) | Primers : OGEM 3 and 4 to got the k1767001 plasmid without LuxR , after doing the PCR we will get a linear DNA so we put two enzymes in the overlaps of the primers to close the plasmids. | PCR to open the j09855 plasmid.Gibson to add Aiia . | - | BBa_K1767001 |
Aug. 2nd to Aug. 6th (2015) | - | We got p0440 part from the distribution kit. We built BBa_K1767002 and BBa_K1767004 Constructs by restriction enzymes. PCR to get the k1767001 plasmid without LuxR . |
- | BBa_K1767002 BBa_K1767004 |
Aug. 9th to Aug. 13th (2015) | Primers : OGEM 5 and 6 to check if we succeed in the PCR ( Those primers for colony PCR) | We got I13521 part from the distribution kit. We built BBa_K1767003 and BBa_K1767005 Constructs by restriction enzymes. Colony PCR to check if we have LuxR in the plasmid. | - | BBa_K1767003 BBa_K1767005 |
Aug. 16th to Aug. 20th (2015) | - | - | AHL experiment #1 | - |
Aug. 23rd to Aug. 27th (2015) | Primers : OGEM 7 and 8 to check if we have yfp in the plasmid ( for colony PCR ). | We got I13003 part from the distribution kit. | AHL experiment #2 | - |
Aug. 30th to Sept. 9th (2015) | Primers: OGEM 9 and 10 to replaced the RFP with RFP ( with LVA ) | We built BBa_K1767009 and BBa_K1767010 Constructs by restriction enzymes.Colony PCR to check if we have YFP in the plasmid. | - | BBa_K1767009 BBa_K1767010 |
Sept. 6th to Sept. 11th (2015) | - | - | AHL experiment # 3 | - |
preparation
- 1. LB medium
- 2. Miniprep
July
July 12th July 13th July 15th July 20th July 22th July 29th
July 12th
July 13th
July 15th
July 20th
July 22th
July 29th
August
& nbsp;August 2th & nbsp;August 3th & nbsp;August 4th & nbsp;August 5th & nbsp;August 6th & nbsp;August 9th & nbsp;August 10th & nbsp;August 11th & nbsp;August 12th & nbsp;August 13th & nbsp;August 16th & nbsp;August 17th & nbsp;August 18th & nbsp;August 19th & nbsp;August 20th & nbsp;August 23th & nbsp;August 24th & nbsp;August 25th & nbsp;August 26th & nbsp;August 27th & nbsp;August 29th & nbsp;August 30th & nbsp;August 31th