Difference between revisions of "Team:London Biohackspace/lab-book"
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<h3><a href="https://2015.igem.org/Team:London_Biohackspace/protocols/egassembly">SLiCE/<i>Ex vivo</i> DNA Assembly <i> i.e.</i> Cheap Gibson </a></h3> | <h3><a href="https://2015.igem.org/Team:London_Biohackspace/protocols/egassembly">SLiCE/<i>Ex vivo</i> DNA Assembly <i> i.e.</i> Cheap Gibson </a></h3> | ||
<h3><a href="https://2015.igem.org/Team:London_Biohackspace/protocols/filtercomp">Filtration method for competent cells preparation</a></h3> | <h3><a href="https://2015.igem.org/Team:London_Biohackspace/protocols/filtercomp">Filtration method for competent cells preparation</a></h3> | ||
| + | <h3><a href="https://2015.igem.org/Team:London_Biohackspace/experiments/yeast-culturing>CULTURING 'WILD' <i>S. cerevisiae</i></a></h3> | ||
<h3><a href="https://2015.igem.org/Team:London_Biohackspace/experiments/miraculin">Expressing Miraculin in <i>S. cerevisiae</i></a></h3> | <h3><a href="https://2015.igem.org/Team:London_Biohackspace/experiments/miraculin">Expressing Miraculin in <i>S. cerevisiae</i></a></h3> | ||
<h3><a href="https://2015.igem.org/Team:London_Biohackspace/experiments/rna-based-regulation">RNA interference based regulation of RFP in <i>E. coli</i></a></h3> | <h3><a href="https://2015.igem.org/Team:London_Biohackspace/experiments/rna-based-regulation">RNA interference based regulation of RFP in <i>E. coli</i></a></h3> | ||
Revision as of 23:50, 18 September 2015
Protocols
Preparing liquid YEPD media
Preparing YEPD agar plates
Electroporation Transformation of E. coli DH5aplha
Electroporation Transformation of S. cerevisiae
Experiments
SLiCE/Ex vivo DNA Assembly i.e. Cheap Gibson
Filtration method for competent cells preparation
Expressing Miraculin in S. cerevisiae
RNA interference based regulation of RFP in E. coli
Expressing Lycopene in E.coli and S. cerevisiae
Creating Leucine auxotrophic S. cerevisiae strains
