Difference between revisions of "Team:DTU-Denmark/Achievements"

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  >POC of Mage in B.S.
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  >Proof of concept of MAGE in B. subtilis
 
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<li><strong>Protocol for multiple OGRE cycles in B.&nbsp;<em>subtilis </em>168</strong>
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<li><strong>Protocol for multiple MAGE cycles in B.&nbsp;<em>subtilis </em>168</strong>
 
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<li><strong>​</strong>Quantified&nbsp;colony picking to be&nbsp;an ineffective method of quantifying OGRE frequency.</li>
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<li><strong>​</strong>Quantified&nbsp;colony picking to be&nbsp;an ineffective method of quantifying MAGE frequency.</li>
 
<li>Showed that the knockout of mutS has a profound&nbsp;efffect on the transformation frequency compared to the wildtype.</li>
 
<li>Showed that the knockout of mutS has a profound&nbsp;efffect on the transformation frequency compared to the wildtype.</li>
 
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<li><strong>Optimal amount of oligo for OGRE</strong>
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<li><strong>Optimal amount of oligo for MAGE</strong>
 
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<li>The&nbsp;optimal amount of oligo was identified.&nbsp;</li>
 
<li>The&nbsp;optimal amount of oligo was identified.&nbsp;</li>

Revision as of 03:09, 19 September 2015


Overview of Achievements

Scroll down for more

Overview of Achievements

The following overview highlights our achievements:

THIS IS A SUGGESTED LISTING

 

  • MAGE Subtilis
    • Knock out of upp and amyE
      • ​Identified that upp and amyE are not optimal selection targets for knockout mutations.
      • We were not able to duplicate Sun2015 and knock out the upp locus using 2kb ssDNA using oligos.
    • Growth experiment for mutants
      • ​We identified that the growth rate of the B. subtilis with a recombinase grew faster than the wild type. This is conflicting with the results by Sun2015 
      • Identified that the mismatch-repeare knockout did not change growth rate.
    • Protocol for multiple MAGE cycles in B. subtilis 168
      • ​Quantified colony picking to be an ineffective method of quantifying MAGE frequency.
      • Showed that the knockout of mutS has a profound efffect on the transformation frequency compared to the wildtype.
    • Optimal amount of oligo for MAGE
      • The optimal amount of oligo was identified. 
    • OD calculator = Dilution calculator
      • ​We made a calculator that could compute the appropriate dilution from the OD600 measured.

 

 

Technical University of Denmark
Department of Systems Biology
Søltofts Plads 221
2800 Kgs. Lyngby
Denmark
P: +45 45 25 25 25
M: dtu-igem-2015@googlegroups.com