Difference between revisions of "Team:London Biohackspace/experiments/yeast-culturing"
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<p>Over time, beer makers have evolved many different strains of yeast to ferment sugars into a wide variety of beers (and wines!) with a multitude of different properties. We decided to use these some of these strains as the starting point for our own syn-bio beer making adventures. Yeast strains derived from unfiltered bottled beer as well as strain purchased from home brew equipment suppliers were grown on YEPD agar plates for use in all subsequent experiments.</p> | <p>Over time, beer makers have evolved many different strains of yeast to ferment sugars into a wide variety of beers (and wines!) with a multitude of different properties. We decided to use these some of these strains as the starting point for our own syn-bio beer making adventures. Yeast strains derived from unfiltered bottled beer as well as strain purchased from home brew equipment suppliers were grown on YEPD agar plates for use in all subsequent experiments.</p> | ||
<h4>Materials and methods</h4> | <h4>Materials and methods</h4> | ||
| + | <p>Yeast sediment from the bottom of an unfiltered bottle of beer was plated onto a YEPD agar plate using a sterile innoculum loop. Approximately 0.25g of dried yeast purchased from a home brew supplier was diluted in 1.5ml distilled water and plateed using a sterile innoculum loop. Plates were left to grow at room temperature for 2-3 days until visible colonies began to appear.</p> | ||
<img src="https://static.igem.org/mediawiki/2015/1/13/LONBIO-extracting-yeast-from-beer-bottles.png" style="border:solid 1px black;margin-top:5px;"/> | <img src="https://static.igem.org/mediawiki/2015/1/13/LONBIO-extracting-yeast-from-beer-bottles.png" style="border:solid 1px black;margin-top:5px;"/> | ||
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<h4>Results</h4> | <h4>Results</h4> | ||
Revision as of 00:15, 19 September 2015
Over time, beer makers have evolved many different strains of yeast to ferment sugars into a wide variety of beers (and wines!) with a multitude of different properties. We decided to use these some of these strains as the starting point for our own syn-bio beer making adventures. Yeast strains derived from unfiltered bottled beer as well as strain purchased from home brew equipment suppliers were grown on YEPD agar plates for use in all subsequent experiments. Yeast sediment from the bottom of an unfiltered bottle of beer was plated onto a YEPD agar plate using a sterile innoculum loop. Approximately 0.25g of dried yeast purchased from a home brew supplier was diluted in 1.5ml distilled water and plateed using a sterile innoculum loop. Plates were left to grow at room temperature for 2-3 days until visible colonies began to appear.Experiments
CULTURING 'WILD' S. CEREVISIAE
Introduction
Materials and methods
Results
Discussion
