Difference between revisions of "Team:Bielefeld-CeBiTec/Notebook/PlasmidDisplay"
| Line 33: | Line 33: | ||
<img class="img-circle img-notebook-nav" src="data:image/gif;base64,R0lGODlhAQABAIAAAHd3dwAAACH5BAAAAAAALAAAAAABAAEAAAICRAEAOw==" alt="PRIA" width="90" height="90"> | <img class="img-circle img-notebook-nav" src="data:image/gif;base64,R0lGODlhAQABAIAAAHd3dwAAACH5BAAAAAAALAAAAAABAAEAAAICRAEAOw==" alt="PRIA" width="90" height="90"> | ||
| + | |||
| + | <a href="https://2015.igem.org/Team:Bielefeld-CeBiTec/Notebook/Miscellaneous" title="Miscellaneous"><img class="img-circle img-inactive img-notebook-nav" src="data:image/gif;base64,R0lGODlhAQABAIAAAHd3dwAAACH5BAAAAAAALAAAAAABAAEAAAICRAEAOw==" alt="Miscellaneous" width="90" height="90"></a> | ||
Revision as of 18:28, 9 July 2015
Plasmid Repressor Interaction Assay
- Heat shock transformation of Biobricks needed for further assays was performed. Glycerol stocks were generated and the plasmids isolated according to the kits manual. The relevant Biobricks were:
- BBa_K525998: T7 promotor and RBS
- BBa_C0012: lacI repressor with LVA Tag
- BBa_R0010: lac Promotor
- BBa_K592008: lac Operator
- Furthermore a plasmid with a gene coding for a histagged lacI Protein was transformed, which was kindly provided by Stefan Hoffmann.
- Purification of histagged lacI
- Test digest of transformed Biobricks: all clones as expected!
- Midi-preps of BBa_R0010 and BBa_K592008