Difference between revisions of "Team:WPI-Worcester/Results"

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<p><h3>Biofilm Assay Results</h3></p>
 
<p><h3>Biofilm Assay Results</h3></p>
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<p style="text-indent: 2em;"><h10> Ultimately, we tested the effects of 11 untagged and 9 BclA-tagged antifreeze proteins on biofilm formation in EMG2:K λ. Of the 20 antifreeze proteins,12 exhibited biofilm enhancing properties, while 8 exhibited biofilm inhibiting properties. Many of the antifreeze proteins that inhibited biofilm formation were untagged, and were either secreted or internally expressed, while antifreeze proteins that enhanced biofilm formation were typically targeted to the cell surface by the BclA tag. The graphs below show the average OD595 of the crystal violet stain for biofilms formed by EMG2:K λ expressing each of the antifreeze proteins normalized to OD595 of the crystal violet stain for biofilms formed by standard EMG2:K λ. The promoter BBa_J23117 is included on both graphs to demonstrate that incorporation of an empty vector does not impact biofilm formation.</h10></p>
  
 
<p><center><img src="https://static.igem.org/mediawiki/2015/thumb/b/ba/WPIBiolfilmIncrease.png/800px-WPIBiolfilmIncrease.png"/></center></p>
 
<p><center><img src="https://static.igem.org/mediawiki/2015/thumb/b/ba/WPIBiolfilmIncrease.png/800px-WPIBiolfilmIncrease.png"/></center></p>

Revision as of 02:34, 19 September 2015


Results

Biofilm Assay Results

Ultimately, we tested the effects of 11 untagged and 9 BclA-tagged antifreeze proteins on biofilm formation in EMG2:K λ. Of the 20 antifreeze proteins,12 exhibited biofilm enhancing properties, while 8 exhibited biofilm inhibiting properties. Many of the antifreeze proteins that inhibited biofilm formation were untagged, and were either secreted or internally expressed, while antifreeze proteins that enhanced biofilm formation were typically targeted to the cell surface by the BclA tag. The graphs below show the average OD595 of the crystal violet stain for biofilms formed by EMG2:K λ expressing each of the antifreeze proteins normalized to OD595 of the crystal violet stain for biofilms formed by standard EMG2:K λ. The promoter BBa_J23117 is included on both graphs to demonstrate that incorporation of an empty vector does not impact biofilm formation.

Antifreeze proteins that increased biofilm formation relative to standard EMG2:Kλ, plus or minus standard error (line at 1).

Antifreeze proteins that decreased biofilm formation relative to standard EMG2:Kλ, plus or minus standard error (line at 1). Error vars indicate +/- standard error.

Freeze Assay Results