Difference between revisions of "Team:EPF Lausanne/Design"

 
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  We addressed these issues using dCas9-w to build a functional bio-transistor. This catalytically dead version of Cas9 lacks the ability to cleave DNA, but can still bind it in a guided fashion. Recognition of the target sequence is mediated by a guide RNA (gRNA) moiety. We fused dCas9 to a RNA Polymerase (RNAP) recruiting element, the w (omega) subunit. Depending on where dCas9-w binds a promoter, it will either activate or inhibit transcription. At an optimal distance upstream from the transcriptional starting site (TSS), the w subunit recruits RNAP activating the transcription of the associated gene. However, when closer to the TSS, dCas9-w sterically hinders RNAP action, thus inhibiting transcription.
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  We addressed these issues using dCas9-ω to build a functional bio-transistor. This catalytically dead version of Cas9 lacks the ability to cleave DNA, but can still bind it in a guided fashion. Recognition of the target sequence is mediated by a guide RNA (gRNA) moiety. We fused dCas9 to a RNA Polymerase (RNAP) recruiting element, the ω (omega) subunit. Depending on where dCas9-ω binds a promoter, it will either activate or inhibit transcription. At an optimal distance upstream from the transcriptional starting site (TSS), the w subunit recruits RNAP activating the transcription of the associated gene. However, when closer to the TSS, dCas9-ω sterically hinders RNAP action, thus inhibiting transcription.
 
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In a classical biologic circuit, the transistor is composed of a promoter regulating an output gene. The input signal is incorporated by a protein regulating the promoter. In dCas9-w bio-transistors, regulatory proteins are replaced by small gRNAs targeting the promoter. The modularity of  gRNA sequences makes the multiplication of analog transistors possible and faster. Thanks to their similarity, dCas9-w - sgRNA complexes behave in an homogeneous fashion, and the specificity of the gRNA prevents cross-talk between different transistors.  Our team therefore confirmed following features: </br>
+
In a classical biologic circuit, the transistor is composed of a promoter regulating an output gene. The input signal is incorporated by a protein regulating the promoter. In dCas9-ω bio-transistors, regulatory proteins are replaced by small gRNAs targeting the promoter. The modularity of  gRNA sequences makes the multiplication of analog transistors possible and faster. Thanks to their similarity, dCas9-ω - sgRNA complexes behave in an homogeneous fashion, and the specificity of the gRNA prevents cross-talk between different transistors.  Our team therefore confirmed following features: </br>
 
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<h1>Experimental validation</h1>
 
<h1>Experimental validation</h1>
 
<h2> Inducibility of the promoter </h2>
 
<h2> Inducibility of the promoter </h2>
<p>To verify induction and repression by dCas9-w, we targeted a GFP controlling promoter once with an activating sgRNA and once with an inhibitory one. Figure 1 shows the GFP level is increased when placing dCas9-w on the activating position (A). Inhibition (R1 and R2) is slightly higher than basal level of fluorescence. This could be explained by the great tightness of J23117 promoter (link Interlab) and by a small leakage due to the presence of the w subunit. To prove an inhibitory sgRNA triggers fluorescence repression, we tested activating and inhibiting the gene at the same time (A/R1 and A/R2). We saw a clear decrease of fluorescence compared to the fully activated state.
+
<p>To verify induction and repression by dCas9-ω, we targeted a GFP controlling promoter once with an activating sgRNA and once with an inhibitory one. Figure 1 shows the GFP level is increased when placing dCas9-ω on the activating position (A). Inhibition (R1 and R2) is slightly higher than basal level of fluorescence. This could be explained by the great tightness of J23117 promoter (link Interlab) and by a small leakage due to the presence of the subunit. To prove an inhibitory sgRNA triggers fluorescence repression, we tested activating and inhibiting the gene at the same time (A/R1 and A/R2). We saw a clear decrease of fluorescence compared to the fully activated state.
 
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<h2> Chainability </h2>
 
<h2> Chainability </h2>
<p>To test the chainability of biotransistors,  the following construct (FIg. 4) was induced with sgRNA x0 etc. Inducing the upstream transistor successfully resulted in induction of downstream transistor (A_u) to levels comparable to direct activation of J23117_alt (A_alt). We notice that the basal expression of the downstream reporter J23117_alt in chained configuration (B_d) is higher than the basal expression of regular J23117_alt (B_alt). This is probably due to leakage from the upstream promoter, as repressing the upstream promoter (R_u) resulted in lower fluorescent output. We therefore believe that our transistors could be chained to form more complex circuits. As far as we know, chaining dCas9-w-regulated promoters hadn’t been attempted. This might be the first exclusively dCas9-based bio-circuit in E. coli !
+
<p>To test the chainability of biotransistors,  the following construct (FIg. 4) was induced with sgRNA x0 etc. Inducing the upstream transistor successfully resulted in induction of downstream transistor (A_u) to levels comparable to direct activation of J23117_alt (A_alt). We notice that the basal expression of the downstream reporter J23117_alt in chained configuration (B_d) is higher than the basal expression of regular J23117_alt (B_alt). This is probably due to leakage from the upstream promoter, as repressing the upstream promoter (R_u) resulted in lower fluorescent output. We therefore believe that our transistors could be chained to form more complex circuits. As far as we know, chaining dCas9-ω-regulated promoters hadn’t been attempted. This might be the first exclusively dCas9-based bio-circuit in E. coli !
 
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<font size="4"> Find out more in our <a href="https://2015.igem.org/Team:EPF_Lausanne/Results" >Results</a> page </font>
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<font size="7"> Find out more in our <a href="https://2015.igem.org/Team:EPF_Lausanne/Results" >Results</a> page </font>
 
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Latest revision as of 03:51, 19 September 2015

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

Prototype design

Overview

Currently, genetic circuits reproducing digital logic are limited in the multiplication and chaining of logic elements as each step requires a different signaling molecule. This triggers an inhomogeneous response and exposes the logic circuit to crosstalk.


basic regulation


We addressed these issues using dCas9-ω to build a functional bio-transistor. This catalytically dead version of Cas9 lacks the ability to cleave DNA, but can still bind it in a guided fashion. Recognition of the target sequence is mediated by a guide RNA (gRNA) moiety. We fused dCas9 to a RNA Polymerase (RNAP) recruiting element, the ω (omega) subunit. Depending on where dCas9-ω binds a promoter, it will either activate or inhibit transcription. At an optimal distance upstream from the transcriptional starting site (TSS), the w subunit recruits RNAP activating the transcription of the associated gene. However, when closer to the TSS, dCas9-ω sterically hinders RNAP action, thus inhibiting transcription.





In a classical biologic circuit, the transistor is composed of a promoter regulating an output gene. The input signal is incorporated by a protein regulating the promoter. In dCas9-ω bio-transistors, regulatory proteins are replaced by small gRNAs targeting the promoter. The modularity of gRNA sequences makes the multiplication of analog transistors possible and faster. Thanks to their similarity, dCas9-ω - sgRNA complexes behave in an homogeneous fashion, and the specificity of the gRNA prevents cross-talk between different transistors. Our team therefore confirmed following features:

  • Inducibility of the promoter
  • Homogeneity: Mutability of the promoter
  • Orthogonality
  • Chainability

Experimental validation

Inducibility of the promoter

To verify induction and repression by dCas9-ω, we targeted a GFP controlling promoter once with an activating sgRNA and once with an inhibitory one. Figure 1 shows the GFP level is increased when placing dCas9-ω on the activating position (A). Inhibition (R1 and R2) is slightly higher than basal level of fluorescence. This could be explained by the great tightness of J23117 promoter (link Interlab) and by a small leakage due to the presence of the subunit. To prove an inhibitory sgRNA triggers fluorescence repression, we tested activating and inhibiting the gene at the same time (A/R1 and A/R2). We saw a clear decrease of fluorescence compared to the fully activated state.

Fig.1 - Fluorescence median of different regulation states of first reporter plasmid at equilibrium. Results are provided for activation, activation-inhibition for both inhibitors, basal level, double inhibition and negative control (autofluorescence). Units are given in relative fluorescence per OD600. Three biological were measured for each state. Error bars in red stand for standard deviation among replicates.

A
A/R1
A/R2
R2
R1
B
R1/R2
Af


Homogeneity: Mutability of the promoter

Logic gates and more complex circuits are composed of several transistors. Therefore we had to prove the activation/inhibition results we obtained were reproducible with a mutated version of the promoter. We changed the inhibitory and activating sequences on the promoter along with associated gRNAs. Figure 2 shows activation (A_alt) and inhibition (R1_alt and R2_alt) worked as expected. Unlike for the first tested promoter, inhibition is this time lower than the basal level (B_alt) but remains close to it. However, the overall pattern is well conserved.

Fig.2 - On the left-hand side, fluorescence between the initial and mutated promoters was compared. On the right-hand side, the histogram shows the fluorescence median across three biological replicates of different regulation states of the mutated promoter at equilibrium. Results are provided for activation, basal level, inhibition with both inhibitors and negative control (autofluorescence). Units are given in relative fluorescence per OD600. Error bars in grey stand for standard deviation between replicates.

A_alt
R1_alt
R2_alt
B_alt
af_alt


Orthogonality

We tested the effect of non-complementary gRNAs on transistors controlling GFP. The output fluorescence levels are similar between each other and close to the basal level (Fig. 3). This proves inputs are specific to their transistor which is a strong hint at the robustness of our system.

Fig.3 - Fluorescence median of different spurious sgRNA-promoter combinations from 3 biological replicates per sample. Z sgRNAs (original) were tested with mutated promoter while X sgRNAs (mutated) were tested with original promoter. Basal levels for each promoter as well as negative control (autofluorescence) were also provided. Units are given in relative fluorescence per OD600. Error bars represent standard deviation across the replicates.

non specific sgRNAs on PAM rich URS J23119 promoter
non specific sgRNAs on PAM rich URS J23119Alt promoter


Chainability

To test the chainability of biotransistors, the following construct (FIg. 4) was induced with sgRNA x0 etc. Inducing the upstream transistor successfully resulted in induction of downstream transistor (A_u) to levels comparable to direct activation of J23117_alt (A_alt). We notice that the basal expression of the downstream reporter J23117_alt in chained configuration (B_d) is higher than the basal expression of regular J23117_alt (B_alt). This is probably due to leakage from the upstream promoter, as repressing the upstream promoter (R_u) resulted in lower fluorescent output. We therefore believe that our transistors could be chained to form more complex circuits. As far as we know, chaining dCas9-ω-regulated promoters hadn’t been attempted. This might be the first exclusively dCas9-based bio-circuit in E. coli !

Fig.4 – Fluorescence median among three biological replicates for different situation. Results are provided for activation of the downstream promoter, activation of the upstream promoter, basal levels of upstream and downstream promoters, inhibition of downstream promoter and negative control (autofluorescence). Units are given in relative fluorescence per OD600. Error bars stand for standard deviation across the triplicates.

A_u
A_alt
B_d
R-u
B_alt
af



Find out more in our Results page




EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits