Difference between revisions of "Team:Michigan/Notebook/September"
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− | <h2 style="text-align: center"> | + | <h2 style="text-align: center">September</h2> |
+ | <p> | ||
+ | Work plan: | ||
+ | <br><br> | ||
+ | LacZ into Thrombin plasmid: | ||
+ | <br> | ||
+ | <ul> | ||
+ | <li>cloning not working probably because the insert is almost as big as backbone | ||
+ | let’s transform LacZ again</li> | ||
+ | <li transform in vitro LacZ part again</li> | ||
+ | <li>plasmid is ready, digest with HindiIII and A</li> | ||
+ | <li>gel extract plasmid, measure dna concentration</li> | ||
+ | </ul> | ||
+ | <br> | ||
+ | LacZ into Gliadin plasmid: | ||
+ | <br> | ||
+ | Pfldh plasmid with GFP: | ||
+ | <br>measure fluorescence | ||
+ | <br><br> | ||
+ | Things to order: | ||
+ | <br> | ||
+ | <ul> | ||
+ | <li>aptamer and trigger for Gliadin</li> | ||
+ | <li> switch for Gliadin with GFP (check design folder)</li> | ||
+ | <li>PfLDH protein</li> | ||
+ | <li>aptamer and trigger for Pfldh</li> | ||
+ | <li>aptamer and trigger for Kanamycin</li> | ||
+ | </ul> | ||
+ | |||
+ | <img src="http://www.facebook.com/l.php?u=http%3A%2F%2F2015.igem.org%2FFile%3ASwitchThrombin.png&h=IAQEgSM9w" alt="MostImportant" style="width:304px;height:228px;"> | ||
</html> | </html> |
Revision as of 03:48, 19 September 2015
September
Work plan:
LacZ into Thrombin plasmid:
- cloning not working probably because the insert is almost as big as backbone let’s transform LacZ again
- plasmid is ready, digest with HindiIII and A
- gel extract plasmid, measure dna concentration
LacZ into Gliadin plasmid:
Pfldh plasmid with GFP:
measure fluorescence
Things to order:
- aptamer and trigger for Gliadin
- switch for Gliadin with GFP (check design folder)
- PfLDH protein
- aptamer and trigger for Pfldh
- aptamer and trigger for Kanamycin