Difference between revisions of "Team:Michigan/Notebook/September"

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Revision as of 03:50, 19 September 2015

September

Work plan:

LacZ into Thrombin plasmid:

  • cloning not working probably because the insert is almost as big as backbone let’s transform LacZ again
  • transform in vitro LacZ part again
  • plasmid is ready, digest with HindiIII and A
  • gel extract plasmid, measure dna concentration

LacZ into Gliadin plasmid:
Pfldh plasmid with GFP:
measure fluorescence

Things to order:
  • aptamer and trigger for Gliadin
  • switch for Gliadin with GFP (check design folder)
  • PfLDH protein
  • aptamer and trigger for Pfldh
  • aptamer and trigger for Kanamycin
MostImportant