Difference between revisions of "Team:Bielefeld-CeBiTec/Notebook/PlasmidDisplay"
| Line 26: | Line 26: | ||
<div class="text-center"> | <div class="text-center"> | ||
| − | <a href="https://2015.igem.org/Team:Bielefeld-CeBiTec/Notebook/HeavyMetals" title="Heavy Metals"><img class="img-circle img-inactive img-notebook-nav" src="data:image/gif;base64,R0lGODlhAQABAIAAAHd3dwAAACH5BAAAAAAALAAAAAABAAEAAAICRAEAOw==" alt="Heavy metals | + | <a href="https://2015.igem.org/Team:Bielefeld-CeBiTec/Notebook/HeavyMetals" title="Heavy Metals"><img class="img-circle img-inactive img-notebook-nav" src="data:image/gif;base64,R0lGODlhAQABAIAAAHd3dwAAACH5BAAAAAAALAAAAAABAAEAAAICRAEAOw==" alt="Heavy metals"></a> |
| − | <a href="https://2015.igem.org/Team:Bielefeld-CeBiTec/Notebook/DRdrugs" title="Date rape drugs"><img class="img-circle img-inactive img-notebook-nav" src="data:image/gif;base64,R0lGODlhAQABAIAAAHd3dwAAACH5BAAAAAAALAAAAAABAAEAAAICRAEAOw==" alt="Date rape drugs | + | <a href="https://2015.igem.org/Team:Bielefeld-CeBiTec/Notebook/DRdrugs" title="Date rape drugs"><img class="img-circle img-inactive img-notebook-nav" src="data:image/gif;base64,R0lGODlhAQABAIAAAHd3dwAAACH5BAAAAAAALAAAAAABAAEAAAICRAEAOw==" alt="Date rape drugs"></a> |
| − | <a href="https://2015.igem.org/Team:Bielefeld-CeBiTec/Notebook/CFPS" title="CFPS"><img class="img-circle img-inactive img-notebook-nav" src="https://static.igem.org/mediawiki/2015/f/ff/Bielefeld-CeBiTec_Notebook_CFPS.jpg" alt="CFPS | + | <a href="https://2015.igem.org/Team:Bielefeld-CeBiTec/Notebook/CFPS" title="CFPS"><img class="img-circle img-inactive img-notebook-nav" src="https://static.igem.org/mediawiki/2015/f/ff/Bielefeld-CeBiTec_Notebook_CFPS.jpg" alt="CFPS"></a> |
| − | <img class="img-circle img-notebook-nav" src="data:image/gif;base64,R0lGODlhAQABAIAAAHd3dwAAACH5BAAAAAAALAAAAAABAAEAAAICRAEAOw==" alt="PRIA | + | <img class="img-circle img-notebook-nav" src="data:image/gif;base64,R0lGODlhAQABAIAAAHd3dwAAACH5BAAAAAAALAAAAAABAAEAAAICRAEAOw==" alt="PRIA"> |
| − | <a href="https://2015.igem.org/Team:Bielefeld-CeBiTec/Notebook/Miscellaneous" title="Miscellaneous"><img class="img-circle img-inactive img-notebook-nav" src="data:image/gif;base64,R0lGODlhAQABAIAAAHd3dwAAACH5BAAAAAAALAAAAAABAAEAAAICRAEAOw==" alt="Miscellaneous | + | <a href="https://2015.igem.org/Team:Bielefeld-CeBiTec/Notebook/Miscellaneous" title="Miscellaneous"><img class="img-circle img-inactive img-notebook-nav" src="data:image/gif;base64,R0lGODlhAQABAIAAAHd3dwAAACH5BAAAAAAALAAAAAABAAEAAAICRAEAOw==" alt="Miscellaneous"></a> |
Revision as of 11:40, 10 July 2015
Plasmid Repressor Interaction Assay
- Heat shock transformation of Biobricks needed for further assays was performed. Glycerol stocks were generated and the plasmids isolated according to the kits manual. The relevant Biobricks were:
- BBa_K525998: T7 promotor and RBS
- BBa_C0012: lacI repressor with LVA Tag
- BBa_R0010: lac Promotor
- BBa_K592008: lac Operator
- Furthermore a plasmid with a gene coding for a histagged lacI Protein was transformed, which was kindly provided by Stefan Hoffmann.
- Purification of histagged lacI
- Test digest of transformed Biobricks: all clones as expected!
- Midi-preps of BBa_R0010 and BBa_K592008