Difference between revisions of "Team:Bielefeld-CeBiTec/Primers"

Line 116: Line 116:
 
                 <td>cttgggtaccgatcgcagaaagactagTACTAGTAGCGGCCGCTGCAG</td>
 
                 <td>cttgggtaccgatcgcagaaagactagTACTAGTAGCGGCCGCTGCAG</td>
 
             </tr>
 
             </tr>
 +
 +
<tr id="pSB_BlcRfwd_new">
 +
<td>psB_BlcRfwd_new"</td>
 +
<td>CTTGGGTACCGATCGCAGAAAGACTAATACTAGTAGCGGCCGCTGCAG</td>
 +
</tr>
  
 
           <tr id="pSB1C3_BlcR_rev">
 
           <tr id="pSB1C3_BlcR_rev">
Line 126: Line 131:
 
                 <td>TTACGCGTAAAATTCGGGAGCCTTC</td>
 
                 <td>TTACGCGTAAAATTCGGGAGCCTTC</td>
 
             </tr>
 
             </tr>
 +
<tr id="BlcC_rev_new">
 +
<td>BlcC_rev_new</td>
 +
<td>TTAGTCTTTCTGCGATCGGTACCC</td>
 +
</tr>
 +
 +
<tr id="bclR-rv">
 +
<td>bclR-rv"</td>
 +
<td>GTCTTTCTGCGATCGGTACCC</td>
 +
</tr>
 +
 +
<tr id="blcR-CBD-fw">
 +
<td>blcR-CBD-fw</td>
 +
<td>AAGCTTGGGTACCGATCGCAGAAAGACCCCGGCGCCAATCCGCCCGGT</td>
 +
</tr>
  
 
           <tr id="pro_Blc_fwd">
 
           <tr id="pro_Blc_fwd">
Line 146: Line 165:
 
                 <td>tgaactctaatgcgggagaggtcgATGGCTTCCTCCGAAGACGTTAT</td>
 
                 <td>tgaactctaatgcgggagaggtcgATGGCTTCCTCCGAAGACGTTAT</td>
 
             </tr>
 
             </tr>
 +
 +
<tr id="BlcB_mut_fwd">
 +
<td>BlcB_mut_fwd</td>
 +
<td>CCTTTCGAGTTCCGCACCGTTCCCT</td>
 +
</tr>
 +
 +
<tr id="BlcB_mut_rev">
 +
<td>BlcB_mut_rev"</td>
 +
<td>AGGGAACGGTGCGGAACTCGAAAGG</td>
 +
</tr>
 +
 +
<tr id="BlcR-L-sfGFP-rv">
 +
<td>BlcR-L-sfGFP-rv</td>
 +
<td>TCGCCTTTACGCATGCCGGTGTCTTTCTGCGATCGGTACCC</td>
 +
</tr>
 +
 +
<tr id="BlcR-T7-RBS-fw">
 +
<td>BlcR-T7-RBS-fw</td>
 +
<td>TAGAGAAAGAGGAGAAATACTAGATGGGCCAAAG</td>
 +
</tr>
  
  

Revision as of 12:54, 22 July 2015

iGEM Bielefeld 2015


Primers

Name Sequence (5´ → 3´)
Standard primers
SB-prep-2Ea ATGAATTCCAGAAATCATCCTTAGCG
SB-prep-3P-1 GCCGCTGCAGTCCGGCAAAAAA
VF CCACCTGACGTCTAAGAA
VR GTATTACCGCCTTTGAGT
Arsenic biosensor
Ars_1_rev tatgacttaacgaatgtgtaagtgcagaggCGGATAAAACACATCTGAAAATTC
Ars_2_fwd cctctgcacttacacattcgttaagtcataGGTACTAGTAGCGGCC
Ars_3_fwd cattcgttaagtcatatatgtttttgacttGGTACTAGTAGCGGCC
Ars_3_rev tcaaaaacatatatgacttaacgaatgtgtCGGATAAAACACATCTGAAAATTC
arsRhis_arsR_fwd tatagggaaagaggagaaatactagagATGTCATTTCTGTTACCCATC
arsRhis_arsR_rev ctagtattagtggtgatggtgatgatgTTAACTGCAAATGTTCTTACTG
arsRhis_Trp_fwd CATCATCACCATCACCAC
arsRhis_Trp_rev CTCTAGTATTTCTCCTCTTTCC
UTR_arsO_rev ccttcttttttttttttaaagttaaacaaaattatTAAGTCAAAAACATATATGACTTAACG
UTR_noT7_rev ccttcttttttttttttaaagttaaacaaaattattCTCTAGAAGCGGCCGCGA
Date rape drugs
BlcR_fwd ATGGGCCAAAGGGGCCAAGT
BlcR_rev CTAGTCTTTCTGCGATCGGTACCC
pSB1C3_BlcR_fwd cttgggtaccgatcgcagaaagactagTACTAGTAGCGGCCGCTGCAG
psB_BlcRfwd_new" CTTGGGTACCGATCGCAGAAAGACTAATACTAGTAGCGGCCGCTGCAG
pSB1C3_BlcR_rev ttgacacacttggcccctttggcccatctagtaTTTCTCCTCTTTCTCTAGTAGCTAGCACAATAC
BlcC_rev TTACGCGTAAAATTCGGGAGCCTTC
BlcC_rev_new TTAGTCTTTCTGCGATCGGTACCC
bclR-rv" GTCTTTCTGCGATCGGTACCC
blcR-CBD-fw AAGCTTGGGTACCGATCGCAGAAAGACCCCGGCGCCAATCCGCCCGGT
pro_Blc_fwd ATGCACCTTCCTTGACACACT
pro_Blc_rev CATCGACCTCTCCCGCATTAG
K516030proB_rev gggccaagtgtgtcaaggaaggtgcatCTCTAGAAGCGGCCGCGAATTCC
K516030proB_fwd tgaactctaatgcgggagaggtcgATGGCTTCCTCCGAAGACGTTAT
BlcB_mut_fwd CCTTTCGAGTTCCGCACCGTTCCCT
BlcB_mut_rev" AGGGAACGGTGCGGAACTCGAAAGG
BlcR-L-sfGFP-rv TCGCCTTTACGCATGCCGGTGTCTTTCTGCGATCGGTACCC
BlcR-T7-RBS-fw TAGAGAAAGAGGAGAAATACTAGATGGGCCAAAG
gBlocks
GABA_B_pSB1C3_f ataagaaaataccgacagggggatattcacTACTAGTAGCGGCCGCTGC
GABA_B_pSB1C3_r cataaaaaaatcccagtcacaggtgattgaCTCTAGAAGCGGCCGCGA
GABA_R_pSB1C3_f tagttttcaaccctttcagcggaggagccatcgTACTAGTAGCGGCCGCTGC
GABA_R_pSB1C3_r ctgtggattaacacaccacctactttttaaCTCTAGAAGCGGCCGCGA
Trp_sy_pSB1C3_f aaagaagttcatcatcaccatcaccactaaTACTAGTAGCGGCCGCTGC
Trp_sy_pSB1C3_r tctcctctttccctatagtgagtcgtattaCTCTAGAAGCGGCCGCGA
Split primers
Cm_fwd CGGCATCAGCACCTTGTC
Cm_rev TATACGCAAGGCGACAAG