Difference between revisions of "Team:Bielefeld-CeBiTec/Notebook/Miscellaneous"
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<p> This Week we started with the <a href="https://2015.igem.org/Tracks/Measurement/Interlab_study" target="_blank">iGEM 2015 Measurement Interlab Study</a>:</p> | <p> This Week we started with the <a href="https://2015.igem.org/Tracks/Measurement/Interlab_study" target="_blank">iGEM 2015 Measurement Interlab Study</a>:</p> | ||
<ul> | <ul> | ||
| − | <li> | + | <li>Heat shock transformation of BioBricks from the distribution:</li> |
<ul> | <ul> | ||
<li><a href="http://parts.igem.org/Part:BBa_K823005" target="_blank">BBa_K823005</a> (Anderson promoter J23101)</li> | <li><a href="http://parts.igem.org/Part:BBa_K823005" target="_blank">BBa_K823005</a> (Anderson promoter J23101)</li> | ||
| Line 192: | Line 192: | ||
<ul> | <ul> | ||
<li>Colonies from the BioBrick Transformation were used to inoculate over night cultures</li> | <li>Colonies from the BioBrick Transformation were used to inoculate over night cultures</li> | ||
| − | + | <ul><li> in 5 ml LB-Medium (37°C)</li></ul> | |
| − | <li> </li> | + | <li>Mini-preps of KRX(BBa_K823005), KRX(BBa_K823008) and KRX(BBa_K823013) ON-cultures</li> |
| − | <li> </li> | + | <ul><li> Plasmids stored at -20°C</li></ul> |
| + | <li>Second heat shock transformation of chem. competent KRX with BBa_I13504</li> | ||
| + | <ul><li> Colonies were used to inoculate over night cultures (5 ml LB, 37°C)</li></ul> | ||
</ul> | </ul> | ||
</div> | </div> | ||
Revision as of 16:57, 21 July 2015
Miscellaneous
This Week we started with the iGEM 2015 Measurement Interlab Study:
- Heat shock transformation of BioBricks from the distribution:
- BBa_K823005 (Anderson promoter J23101)
- BBa_K823008 (Anderson promoter J23106)
- BBa_K823013 (Anderson promoter J23117)
- BBa_I13504 (GFP)
iGEM 2015 Measurement Interlab Study:
- Colonies from the BioBrick Transformation were used to inoculate over night cultures
- in 5 ml LB-Medium (37°C)
- Mini-preps of KRX(BBa_K823005), KRX(BBa_K823008) and KRX(BBa_K823013) ON-cultures
- Plasmids stored at -20°C
- Second heat shock transformation of chem. competent KRX with BBa_I13504
- Colonies were used to inoculate over night cultures (5 ml LB, 37°C)