Difference between revisions of "Team:Paris Bettencourt/Notebook/VitaminA"
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<h3>Procedure</h3> | <h3>Procedure</h3> | ||
| − | < | + | <ol> |
<li>Liquid culture overnight in LB + Ampicillin.</li> | <li>Liquid culture overnight in LB + Ampicillin.</li> | ||
<li>Made a glycerol stock and stored it in the -20 freezer (g15.35)</li> | <li>Made a glycerol stock and stored it in the -20 freezer (g15.35)</li> | ||
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<li>Measure concentration with Nanodrop</li> | <li>Measure concentration with Nanodrop</li> | ||
| − | </ | + | </ol> |
<h3>Results</h3> | <h3>Results</h3> | ||
Revision as of 10:29, 23 July 2015
Jul. 14th
Goal
Extract the integrative plasmid HO-Poly-KanMX4-HO from the E.Coli provided by AddGene (Accession number #51662).Procedure
- Liquid culture overnight in LB + Ampicillin.
- Made a glycerol stock and stored it in the -20 freezer (g15.35)
- Centrifuge the tube for 1 minute with 11000 rpm.
- Follow the standard protocol of Thermo Scientific miniprep kit (is it? I followed Ihab's protocol here: https://docs.google.com/document/d/1QHBHcN8RY0c-oEY8X5kAqPrKYODTBm7K8eU_lY_yw-o/edit, we should compare them)
- Measure concentration with Nanodrop
Results
Final DNA concentrations of the 4 tubes of miniprep, measured with Nanodrop:- tube HO pl. 1 = 431.1 ng/uL
- tube HO pl. 2 = 313.6 ng/uL
- tube HO pl. 3 = 366.4 ng/uL
- tube HO pl. 4 = 261.7 ng/uL
- Made the project, it is awesome!
Jul. 24th