Difference between revisions of "Team:UNITN-Trento/Test"

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<h2>Electron production with <strong><i>E. coli</i></strong> and proteorhodopsin </h2>
 
<h2>Electron production with <strong><i>E. coli</i></strong> and proteorhodopsin </h2>
 
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<p>The aim of our project is to develop a pseudoautotrophic <span class="i_enph" style="font-style:italic;">E. coli</span>, enabling it to live in the microbial fuel cell anaerobic condition. Our idea is to engineer E. coli with proteorhodopsin, a light-powered proton pump identified in bacteria that live in deep waters. This protein is activated by light and needs retinal molecules to pump protons outward following excitation. Thus we want to engineer our bacteria with blh, that encodes for the enzyme that cleaves beta-carotene and produces retinal.</p>
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<p>Hi guys!
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<p>To increase electron production we plan to overexpress pncB that represents the limiting step in the NAD+ synthesis pathway. The more NAD+ is produced, the more electrons are transported by NADH. In short: light activates proteorhodopsin, increases bacterial viability, and drives to a more efficient electron production. Our bacterial device will give rise to a self-sustainable system, powered by sunlight during the day and by a light bulb at night. Daytime-electricity can be either used or stored to be employed as a power source for the lightbulb at night.</p>
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We are the UNITN-Trento iGEM team 2015, here is our wonderful idea to change the way people think about Microbial Fuel Cells.
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We attempted to improve MFC platform by exploiting the power of the sun and the <span class="i_enph"<i>E. coli</i></span> versatility, so we added new interesting features to build a “Solar pMFC”. Check out our wiki page to learn more about it!</p>
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<p class="pintest">Electricity from E. coli<br/>
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<span>Check out our amazing idea! Have fun!<br/>
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<a class="btn" href="#">Project</a></span></p>
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<p class="pintest">Electricity from E. coli<br/>
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<span>Check out our amazing idea! Have fun!<br/>
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<a class="btn" href="#">Project</a></span></p>
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<li style="width: 25%; background-image: url('https://static.igem.org/mediawiki/2015/f/fc/Unitn_pics_slider_3.jpg'); ">
 
<li style="width: 25%; background-image: url('https://static.igem.org/mediawiki/2015/f/fc/Unitn_pics_slider_3.jpg'); ">
 
<p class="pintest">Electricity from E. coli<br/>
 
<p class="pintest">Electricity from E. coli<br/>
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<p class="pintest">Meet the team!<br/>
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<p class="pintest">UNITN-iGEM Team 2015<br/>
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<span>We are UNITN iGEM 2015 and we are such essence as dNTPs are made on<br/>
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<span>Click here to meet the team!<br/>
 
 
 
<a class="btn" href="https://2015.igem.org/Team:UNITN-Trento/Team"> Team </a></span></p>
 
<a class="btn" href="https://2015.igem.org/Team:UNITN-Trento/Team"> Team </a></span></p>
 
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<p class="pintest">Catch 'em all!<br/>
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<p class="pintest">Interlab Measurement Study<br/>
 
<span>Many techniques, different strains and so much fluorescence… Enjoy the Measurement Interlab Study<br/>
 
<span>Many techniques, different strains and so much fluorescence… Enjoy the Measurement Interlab Study<br/>
 
 
 
<a class="btn" href="#"> Interlab Study </a></span></p>
 
<a class="btn" href="#"> Interlab Study </a></span></p>
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<p class="pintest">Energy waste is a serious problem!<br/>
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<span>Follow us and find out how to save it<br/>
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<a class="btn" href="#"> Human Practices </a></span></p>
 
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Revision as of 16:12, 12 August 2015

Electron production with E. coli and proteorhodopsin


Hi guys! We are the UNITN-Trento iGEM team 2015, here is our wonderful idea to change the way people think about Microbial Fuel Cells. We attempted to improve MFC platform by exploiting the power of the sun and the E. coli versatility, so we added new interesting features to build a “Solar pMFC”. Check out our wiki page to learn more about it!

Achievements

8 new BioBricks

We built and submitted a total number of new BioBricks. Check out our Parts page to know more!

Improved 2 Parts

Something existing, reshaped better. Now they work better!

Measurements Interlab Study

We are participating also to the Measurements Interlab Study. Check out our progress in the Interlab Section!