Difference between revisions of "Team:Bielefeld-CeBiTec/Notebook/PlasmidDisplay"
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Revision as of 19:13, 16 August 2015
Plasmid Repressor Interaction Assay
- Heat shock transformation of Biobricks needed for further assays was performed. Glycerol stocks were generated and the plasmids isolated according to the kits manual. The relevant Biobricks were:
- BBa_K525998: T7 promotor and RBS
- BBa_C0012: lacI repressor with LVA Tag
- BBa_R0010: lac Promotor
- BBa_K592008: lac Operator
- Furthermore a plasmid with a gene coding for a histagged lacI Protein was transformed, which was kindly provided by Stefan Hoffmann.
- Purification of histagged lacI
- Test digest of transformed Biobricks: all clones as expected!
- Midi-preps of BBa_R0010 and BBa_K592008