Difference between revisions of "Team:Dundee/Sequences"
| Line 20: | Line 20: | ||
<a class="anchor" id="top"></a> | <a class="anchor" id="top"></a> | ||
<center> | <center> | ||
| − | <h1><highlight class="highlight">Sequences</highlight></h1 | + | <h1><highlight class="highlight">Sequences and Parts</highlight></h1> |
| − | + | ||
</center> | </center> | ||
</header> | </header> | ||
| − | + | <div class="ribbon"> | |
| + | <div class="rows"> | ||
<!-- Blood --> | <!-- Blood --> | ||
| Line 32: | Line 32: | ||
<div class="border-week"> | <div class="border-week"> | ||
<div class="box"> | <div class="box"> | ||
| − | <div class="labtitle">Sequences for Blood-detector</div> | + | <div class="labtitle">Sequences for the Blood-detector</div> |
<span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew1"></span> | <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew1"></span> | ||
| + | |||
<div class="box-content"> | <div class="box-content"> | ||
| − | + | ||
| − | + | <p class="journal-summary-heading">Detecting Blood</p> | |
| − | + | <p class="journal-content">The biobricks in this section are designed for the detection of blood. All sequences are synthetically optimised for expression in our chassis.</p> | |
| + | |||
| + | <div id="collapsiblew1" class="collapse week-content"> | ||
<!-- when copying for next week, make sure to change labtitle, span, collapsible in last line. In each separate day change span and following div--> | <!-- when copying for next week, make sure to change labtitle, span, collapsible in last line. In each separate day change span and following div--> | ||
| + | |||
| + | <!-- This range of part numbers has been assigned to your team for use during the summer: BBa_K1590000 to BBa_K1590999 --> | ||
| Line 47: | Line 52: | ||
<div class="border-day"> | <div class="border-day"> | ||
<div class="box"> | <div class="box"> | ||
| − | <span class="box-title">Haemoglobin A</ | + | <span class="box-title"><a href="http://parts.igem.org/Part:BBa_K1590000" target="_blank">Haemoglobin A</a></span> |
<span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew1-1"></span> | <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew1-1"></span> | ||
<div id="collapsiblew1-1" class="collapse box-content sequences"> | <div id="collapsiblew1-1" class="collapse box-content sequences"> | ||
| − | <p>> | + | <p>>BBa_K1590000 (Haemoglobin A)<br> |
| − | + | ATGGTTCTGTCTCCGGCGGACAAAACCAACGTTAAAGCGGCGTGGGGTAAAGTTGGTGCGCACGCGGGTGAATACGGTGCGGAAGCGCTGGAACGTATGTTCCTGTCTTTCCCGACCACCAAAACCTACTTCCCGCACTTCGACCTGTCTCACGGTTCTGCGCAGGTTAAAGGTCACGGTAAAAAAGTTGCGGACGCGCTGACCAACGCGGTTGCGCACGTTGACGACATGCCGAACGCGCTGTCTGCGCTGTCTGACCTGCACGCGCACAAACTGCGTGTTGACCCGGTTAACTTCAAACTGCTGTCTCACTGCCTGCTGGTTACCCTGGCGGCGCACCTGCCGGCGGAGTTCACCCCGGCGGTTCACGCGTCTCTGGACAAATTCCTGGCGTCTGTTTCTACCGTTCTGACCTCTAAATACCGTTAA</p> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</div> | </div> | ||
| Line 67: | Line 68: | ||
<div class="border-day"> | <div class="border-day"> | ||
<div class="box"> | <div class="box"> | ||
| − | <span class="box-title">Haemoglobin | + | <span class="box-title"><a href="http://parts.igem.org/Part:BBa_K1590001" target="_blank">Haemoglobin B</a></span> |
<span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew1-2"></span> | <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew1-2"></span> | ||
<div id="collapsiblew1-2" class="collapse box-content sequences"> | <div id="collapsiblew1-2" class="collapse box-content sequences"> | ||
| − | <p>> | + | <p>>BBa_K1590001 (Haemoglobin B)<br> |
| − | + | ATGGTTCACCTGACCCCGGAAGAAAAATCTGCGGTTACCGCGCTGTGGGGTAAAGTTAACGTTGACGAAGTTGGTGGTGAAGCGCTGGGTCGTCTGCTGGTTGTTTACCCGTGGACCCAGCGTTTCTTCGAATCTTTCGGTGACCTGTCTACCCCGGACGCGGTTATGGGTAACCCGAAAGTTAAAGCGCACGGTAAAAAAGTTCTGGGTGCGTTCTCTGACGGTCTGGCGCACCTGGACAACCTGAAAGGCACCTTCGCGACCCTGTCTGAACTGCACTGCGACAAACTGCACGTTGACCCGGAAAACTTCCGTCTGCTGGGTAACGTTCTGGTTTGCGTTCTGGCGCACCACTTCGGTAAAGAGTTCACCCCGCCGGTTCAGGCGGCGTACCAGAAAGTTGTTGCGGGTGTTGCGAACGCGCTGGCGCACAAATACCACTAA</p> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</div> | </div> | ||
| Line 87: | Line 84: | ||
<div class="border-day"> | <div class="border-day"> | ||
<div class="box"> | <div class="box"> | ||
| − | <span class="box-title"> | + | <span class="box-title"><a href="http://parts.igem.org/Part:BBa_K1590002" target="_blank">Haptoglobin</a></span> |
<span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew1-3"></span> | <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew1-3"></span> | ||
<div id="collapsiblew1-3" class="collapse box-content sequences"> | <div id="collapsiblew1-3" class="collapse box-content sequences"> | ||
| − | <p>> | + | <p>>BBa_K1590002 (Haptoglobin)<br> |
| − | + | ATGTCTGCGCTGGGTGCGGTTATCGCGCTGCTGCTGTGGGGTCAGCTGTTCGCGGTTGACTCTGGTAACGACGTTACCGACATCGCGGACGACGGTTGCCCGAAACCGCCGGAAATCGCGCACGGTTACGTTGAACACTCTGTTCGTTACCAGTGCAAAAACTACTACAAACTGCGTACCGAAGGTGACGGTGTTTACACCCTGAACGACAAAAAACAGTGGATCAACAAAGCAGTAGGCGACAAACTGCCGGAATGCGAAGCCGATGACGGCTGTCCGAAACCACCGGAGATCGCTCATGGCTACGTCGAACACTCGGTGCGTTATCAGTGCAAAAACTACTACAAACTGCGCACGGAAGGAGACGGGGTGTACACTCTTAATAACGAGAAACAGTGGATTAACAAAGCGGTTGGCGATAAGCTGCCGGAATGTGAGGCAGTCTGCGGTAAACCGAAAAACCCGGCGAACCCGGTTCAGCGTATCTTGGGCGGGCACTTAGATGCGAAAGGTTCATTCCCGTGGCAGGCCAAAATGGTTTCCCACCACAATCTCACGACAGGGGCGACCCTGATCAACGAGCAGTGGTTGCTGACCACTGCTAAAAATCTGTTTCTGAACCATAGCGAAAATGCGACGGCGAAAGACATCGCGCCGACCCTGACCCTGTACGTTGGTAAAAAACAGCTGGTTGAAATCGAAAAAGTTGTTCTGCACCCGAACTACTCTCAGGTTGACATCGGTCTGATCAAACTGAAACAGAAAGTTTCTGTTAACGAACGTGTTATGCCGATCTGCCTGCCGTCTAAAGACTACGCGGAAGTTGGTCGTGTTGGTTACGTTTCTGGTTGGGGTCGTAACGCGAACTTCAAATTCACCGACCACCTGAAATACGTTATGCTGCCGGTTGCGGACCAGGACCAGTGCATCCGTCACTACGAAGGTTCTACCGTTCCGGAAAAAAAAACCCCGAAATCTCCGGTTGGTGTTCAGCCGATCCTGAACGAACACACCTTCTGCGCGGGTATGTCTAAATACCAGGAAGACACCTGCTACGGTGACGCGGGTTCTGCGTTCGCGGTTCACGACCTGGAAGAAGACACCTGGTACGCGACCGGTATCCTGTCTTTCGACAAATCTTGCGCGGTTGCGGAATACGGTGTTTACGTTAAAGTTACCTCTATCCAGGACTGGGTTCAGAAAACCATCGCGGAAAACTAA</p> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</div> | </div> | ||
| Line 128: | Line 113: | ||
<div class="labtitle">Sequences for the Chromate-detector</div> | <div class="labtitle">Sequences for the Chromate-detector</div> | ||
<span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew2"></span> | <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew2"></span> | ||
| + | |||
<div class="box-content"> | <div class="box-content"> | ||
| + | |||
| + | <p class="journal-summary-heading">Detecting Chromate</p> | ||
| + | <p class="journal-content">This set of biobricks is based on an operon of ochrobactrum tritici, conferring resistance against Cr(VI). The Beijing institute of Biotechnology already submitted a biobrick based on this operon in 2013, <a href="http://parts.igem.org/Part:BBa_K1058008" target="_blank">BBa_K1058008</a> (See Figure 1). We used this brick, broke it down to its constituent parts, and reassembled it in a different way (See Figure 2).</p> | ||
| + | |||
| + | <figure> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/a/a0/Plasmid_map_BBa_K1058008.png" alt="Figure1 - Plasmid map of BBa_K1058008." class="plasmidimg"> | ||
| + | <figcaption>Figure 1: Plasmid map of <a href="http://parts.igem.org/Part:BBa_K1058008" target="_blank">BBa_K1058008</a><br> by BIT2013.</figcaption> | ||
| + | </figure> | ||
| + | |||
| + | <figure> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/d/d4/Dundee15_plasmid_map_pChrGFP.png" alt="Figure2 - Plasmid map after reassembling parts." class="plasmidimg"> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/c/c1/Dundee15_plasmid_map_ChrB.png" alt="Figure2 - Plasmid map after reassembling parts." class="plasmidimg"> | ||
| + | <figcaption> Figure 2: Plasmid maps of BBa_K1590006 (pChrGFP) and BBa_K1590009 (ChrB).<br> | ||
| + | These 2 biobricks are transformed into one cell in order to rebuild a chromate sensor.</figcaption> | ||
| + | </figure> | ||
| + | |||
| + | <p><h3>Recommended reading:</h3> | ||
| + | <ul class="citation"> | ||
| + | <li><a href="http://jb.asm.org/content/190/21/6996.full" target="_blank">Branco R et al. The Chromate-Inducible chrBACF Operon from the Transposable Element TnOtChr Confers Resistance to Chromium(VI) and Superoxide. Journal of Bacteriology. 2008;190(21):6996-7003.</a></li> | ||
| + | <li><a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3543429/" target="_blank">Branco R et al. Highly Sensitive, Highly Specific Whole-Cell Bioreporters for the Detection of Chromate in Environmental Samples. PloS one. 2013;8(1):e54005.</a></li> | ||
| + | </ul> | ||
| + | </p> | ||
| + | |||
| + | </div> | ||
</div> | </div> | ||
| Line 141: | Line 151: | ||
<div class="border-day"> | <div class="border-day"> | ||
<div class="box"> | <div class="box"> | ||
| − | <span class="box-title">BBa_K1058008</ | + | <span class="box-title"><a href="http://parts.igem.org/Part:BBa_K1058008" target="_blank">pChrGFP by BIT 2013</a></span> |
<span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew2-1"></span> | <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew2-1"></span> | ||
<div id="collapsiblew2-1" class="collapse box-content sequences"> | <div id="collapsiblew2-1" class="collapse box-content sequences"> | ||
<p>>BBa_K1058008<br> | <p>>BBa_K1058008<br> | ||
| − | + | ATTGCTTATTCCTATTGCCATTTGCTTTTATTTTGCAATACGTTTTAGCAACGCACAACACCTTATGGGTGTGCTGCTTCCATTGTGATTGCGCAAATGTCCGTTTTTGCAATCTACTCAAGACTTTATTTCGTAGATCTTATCTCATTATTGTAGTAACATCTACGACATGAATTTGCTTTCGCCTATCCTTTCCTTGCCTACTGAAAACGCGACCGTCCGGCAACGGACGTGGCGTGCCCTCAAAGCCTCCGGCGCTGCGGTGCTGCGCGACGGCGTGTACCTGATGCCCGACCGCGACGAATGCCGCGCGGTGCTGGATAACTTGGCCTCCGATGTGCGTGAAGGCGGTGGGGTCGCTCATGTGCTGCGTATGGAAGACCCTGAAGGGGTCAACTTCGTCGCATTATTCGACCGCAGCAACGATTTTGCTGCCTTGCTGGTCGATGTCCATCATCTCAGGCAGACGCTGACATTGGATACCGTGCAAGACGTGCTGCGGCAGGTGCGTAAGCTTCGCAAATCCTTTACTACGCTGGTTGAAATCGACTTCTACCCCGGCGAGGCTCAGCGTCAAGCGGACAGTGCGTTGTGTGAACTGGAACAAGCTTGCGCCCGCACGCTGTCGCCGGACGAGCCGCACGCTGTAGAGGGGACTATTACCCGCTTGGATCGTTTGGATTACCAGGCCCGTACCTGGGCCACTCGCGCACGTCCTTGGGTTGATCGGCTCGCCAGCGCATGGCTGATCCGGCGCTTCATCGACCCGCAGGCGCGCATCCTTTGGTTGGCAACCCCTGCGGACTGCCCGCCGGATGCGTTGGGCTTCGACTTCGATGGCGCGACGTTCAGCCATGTCGGCAGCCGTGTCACGTTCGAGGTCCTGGCGGCGAGCTTTGGGCTGGAACAGCCCGCCATCACAAGGATTGGCCTTGTGGTGCATTACCTCGACGTGGGCGGCATCCAGCCGCCAGAGGCCACTGGTATCGAAAGCGTACTGGCCGGTTTGCGGGAAACGGTTGACCACGACGATCAACTATTGGCCATCGCCTCCACTGTGTTCGATGGCTTACTGGCCAGCTTTGAAAAAGGGACCCTCACCGTATGAGGATCCGAAGGAGATATACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTA</p> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</div> | </div> | ||
| Line 175: | Line 167: | ||
<div class="border-day"> | <div class="border-day"> | ||
<div class="box"> | <div class="box"> | ||
| − | <span class="box-title">pChr</ | + | <span class="box-title"><a href="http://parts.igem.org/Part:BBa_K1590003" target="_blank">pChr</a></span> |
<span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew2-2"></span> | <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew2-2"></span> | ||
<div id="collapsiblew2-2" class="collapse box-content sequences"> | <div id="collapsiblew2-2" class="collapse box-content sequences"> | ||
| − | <p>>pChr<br> | + | <p>>BBa_K1590003 (pChr)<br> |
ATTGCTTATTCCTATTGCCATTTGCTTTTATTTTGCAATACGTTTTAGCAACGCACAACACCTTATGGGTGTGCTGCTTCCATTGTGATTGCGCAAATGTCCGTTTTTGCAATCTACTCAAGACTTTATTTCGTAGATCTTATCTCATTATTGTAGTAACATCTACGAC</p> | ATTGCTTATTCCTATTGCCATTTGCTTTTATTTTGCAATACGTTTTAGCAACGCACAACACCTTATGGGTGTGCTGCTTCCATTGTGATTGCGCAAATGTCCGTTTTTGCAATCTACTCAAGACTTTATTTCGTAGATCTTATCTCATTATTGTAGTAACATCTACGAC</p> | ||
| Line 191: | Line 183: | ||
<div class="border-day"> | <div class="border-day"> | ||
<div class="box"> | <div class="box"> | ||
| − | <span class="box-title">ChrB</ | + | <span class="box-title"><a href="http://parts.igem.org/Part:BBa_K1590004" target="_blank">ChrB</a></span> |
<span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew2-3"></span> | <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew2-3"></span> | ||
<div id="collapsiblew2-3" class="collapse box-content sequences"> | <div id="collapsiblew2-3" class="collapse box-content sequences"> | ||
| − | <p>>ChrB<br> | + | <p>>BBa_K1590004 (ChrB)<br> |
ATGAATTTGCTTTCGCCTATCCTTTCCTTGCCTACTGAAAACGCGACCGTCCGGCAACGGACGTGGCGTGCCCTCAAAGCCTCCGGCGCTGCGGTGCTGCGCGACGGCGTGTACCTGATGCCCGACCGCGACGAATGCCGCGCGGTGCTGGATAACTTGGCCTCCGATGTGCGTGAAGGCGGTGGGGTCGCTCATGTGCTGCGTATGGAAGACCCTGAAGGGGTCAACTTCGTCGCATTATTCGACCGCAGCAACGATTTTGCTGCCTTGCTGGTCGATGTCCATCATCTCAGGCAGACGCTGACATTGGATACCGTGCAAGACGTGCTGCGGCAGGTGCGTAAGCTTCGCAAATCCTTTACTACGCTGGTTGAAATCGACTTCTACCCCGGCGAGGCTCAGCGTCAAGCGGACAGTGCGTTGTGTGAACTGGAACAAGCTTGCGCCCGCACGCTGTCGCCGGACGAGCCGCACGCTGTAGAGGGGACTATTACCCGCTTGGATCGTTTGGATTACCAGGCCCGTACCTGGGCCACTCGCGCACGTCCTTGGGTTGATCGGCTCGCCAGCGCATGGCTGATCCGGCGCTTCATCGACCCGCAGGCGCGCATCCTTTGGTTGGCAACCCCTGCGGACTGCCCGCCGGATGCGTTGGGCTTCGACTTCGATGGCGCGACGTTCAGCCATGTCGGCAGCCGTGTCACGTTCGAGGTCCTGGCGGCGAGCTTTGGGCTGGAACAGCCCGCCATCACAAGGATTGGCCTTGTGGTGCATTACCTCGACGTGGGCGGCATCCAGCCGCCAGAGGCCACTGGTATCGAAAGCGTACTGGCCGGTTTGCGGGAAACGGTTGACCACGACGATCAACTATTGGCCATCGCCTCCACTGTGTTCGATGGCTTACTGGCCAGCTTTGAAAAAGGGACCCTCACCGTATGA</p> | ATGAATTTGCTTTCGCCTATCCTTTCCTTGCCTACTGAAAACGCGACCGTCCGGCAACGGACGTGGCGTGCCCTCAAAGCCTCCGGCGCTGCGGTGCTGCGCGACGGCGTGTACCTGATGCCCGACCGCGACGAATGCCGCGCGGTGCTGGATAACTTGGCCTCCGATGTGCGTGAAGGCGGTGGGGTCGCTCATGTGCTGCGTATGGAAGACCCTGAAGGGGTCAACTTCGTCGCATTATTCGACCGCAGCAACGATTTTGCTGCCTTGCTGGTCGATGTCCATCATCTCAGGCAGACGCTGACATTGGATACCGTGCAAGACGTGCTGCGGCAGGTGCGTAAGCTTCGCAAATCCTTTACTACGCTGGTTGAAATCGACTTCTACCCCGGCGAGGCTCAGCGTCAAGCGGACAGTGCGTTGTGTGAACTGGAACAAGCTTGCGCCCGCACGCTGTCGCCGGACGAGCCGCACGCTGTAGAGGGGACTATTACCCGCTTGGATCGTTTGGATTACCAGGCCCGTACCTGGGCCACTCGCGCACGTCCTTGGGTTGATCGGCTCGCCAGCGCATGGCTGATCCGGCGCTTCATCGACCCGCAGGCGCGCATCCTTTGGTTGGCAACCCCTGCGGACTGCCCGCCGGATGCGTTGGGCTTCGACTTCGATGGCGCGACGTTCAGCCATGTCGGCAGCCGTGTCACGTTCGAGGTCCTGGCGGCGAGCTTTGGGCTGGAACAGCCCGCCATCACAAGGATTGGCCTTGTGGTGCATTACCTCGACGTGGGCGGCATCCAGCCGCCAGAGGCCACTGGTATCGAAAGCGTACTGGCCGGTTTGCGGGAAACGGTTGACCACGACGATCAACTATTGGCCATCGCCTCCACTGTGTTCGATGGCTTACTGGCCAGCTTTGAAAAAGGGACCCTCACCGTATGA</p> | ||
| Line 207: | Line 199: | ||
<div class="border-day"> | <div class="border-day"> | ||
<div class="box"> | <div class="box"> | ||
| − | <span class="box-title">Optimised ChrB</ | + | <span class="box-title"><a href="http://parts.igem.org/Part:BBa_K1590005" target="_blank">Optimised ChrB</a></span> |
<span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew2-4"></span> | <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew2-4"></span> | ||
<div id="collapsiblew2-4" class="collapse box-content sequences"> | <div id="collapsiblew2-4" class="collapse box-content sequences"> | ||
| − | <p>> | + | <p>>BBa_K1590005 (Partially optimised ChrB)<br> |
ATGAACCTGCTGTCTCCGATCCTGTCTCTGCCGACCGAAAACGCGACCGTCCGGCAACGGACGTGGCGTGCCCTCAAAGCCTCCGGCGCTGCGGTGCTGCGCGACGGCGTGTACCTGATGCCCGACCGCGACGAATGCCGCGCGGTGCTGGATAACTTGGCCTCCGATGTGCGTGAAGGCGGTGGGGTCGCTCATGTGCTGCGTATGGAAGACCCTGAAGGGGTCAACTTCGTCGCATTATTCGACCGCAGCAACGATTTTGCTGCCTTGCTGGTCGATGTCCATCATCTCAGGCAGACGCTGACATTGGATACCGTGCAAGACGTGCTGCGGCAGGTGCGTAAGCTTCGCAAATCCTTTACTACGCTGGTTGAAATCGACTTCTACCCCGGCGAGGCTCAGCGTCAAGCGGACAGTGCGTTGTGTGAACTGGAACAAGCTTGCGCCCGCACGCTGTCGCCGGACGAGCCGCACGCTGTAGAGGGGACTATTACCCGCTTGGATCGTTTGGATTACCAGGCCCGTACCTGGGCCACTCGCGCACGTCCTTGGGTTGATCGGCTCGCCAGCGCATGGCTGATCCGGCGCTTCATCGACCCGCAGGCGCGCATCCTTTGGTTGGCAACCCCTGCGGACTGCCCGCCGGATGCGTTGGGCTTCGACTTCGATGGCGCGACGTTCAGCCATGTCGGCAGCCGTGTCACGTTCGAGGTCCTGGCGGCGAGCTTTGGGCTGGAACAGCCCGCCATCACAAGGATTGGCCTTGTGGTGCATTACCTCGACGTGGGCGGCATCCAGCCGCCAGAGGCCACTGGTATCGAAAGCGTACTGGCCGGTTTGCGGGAAACGGTTGACCACGACGATCAACTATTGGCCATCGCCTCCACTGTGTTCGATGGCTTACTGGCCAGCTTTGAAAAAGGGACCCTCACCGTATGA</p> | ATGAACCTGCTGTCTCCGATCCTGTCTCTGCCGACCGAAAACGCGACCGTCCGGCAACGGACGTGGCGTGCCCTCAAAGCCTCCGGCGCTGCGGTGCTGCGCGACGGCGTGTACCTGATGCCCGACCGCGACGAATGCCGCGCGGTGCTGGATAACTTGGCCTCCGATGTGCGTGAAGGCGGTGGGGTCGCTCATGTGCTGCGTATGGAAGACCCTGAAGGGGTCAACTTCGTCGCATTATTCGACCGCAGCAACGATTTTGCTGCCTTGCTGGTCGATGTCCATCATCTCAGGCAGACGCTGACATTGGATACCGTGCAAGACGTGCTGCGGCAGGTGCGTAAGCTTCGCAAATCCTTTACTACGCTGGTTGAAATCGACTTCTACCCCGGCGAGGCTCAGCGTCAAGCGGACAGTGCGTTGTGTGAACTGGAACAAGCTTGCGCCCGCACGCTGTCGCCGGACGAGCCGCACGCTGTAGAGGGGACTATTACCCGCTTGGATCGTTTGGATTACCAGGCCCGTACCTGGGCCACTCGCGCACGTCCTTGGGTTGATCGGCTCGCCAGCGCATGGCTGATCCGGCGCTTCATCGACCCGCAGGCGCGCATCCTTTGGTTGGCAACCCCTGCGGACTGCCCGCCGGATGCGTTGGGCTTCGACTTCGATGGCGCGACGTTCAGCCATGTCGGCAGCCGTGTCACGTTCGAGGTCCTGGCGGCGAGCTTTGGGCTGGAACAGCCCGCCATCACAAGGATTGGCCTTGTGGTGCATTACCTCGACGTGGGCGGCATCCAGCCGCCAGAGGCCACTGGTATCGAAAGCGTACTGGCCGGTTTGCGGGAAACGGTTGACCACGACGATCAACTATTGGCCATCGCCTCCACTGTGTTCGATGGCTTACTGGCCAGCTTTGAAAAAGGGACCCTCACCGTATGA</p> | ||
| Line 239: | Line 231: | ||
<div class="border-day"> | <div class="border-day"> | ||
<div class="box"> | <div class="box"> | ||
| − | <span class="box-title"> | + | <span class="box-title"><a href="http://parts.igem.org/Part:BBa_K1590006" target="_blank">pChrGFP</a></span> |
<span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew2-6"></span> | <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew2-6"></span> | ||
<div id="collapsiblew2-6" class="collapse box-content sequences"> | <div id="collapsiblew2-6" class="collapse box-content sequences"> | ||
| − | <p>>pChrGFP<br> | + | <p>>BBa_K1590006 (pChrGFP)<br> |
ATTGCTTATTCCTATTGCCATTTGCTTTTATTTTGCAATACGTTTTAGCAACGCACAACACCTTATGGGTGTGCTGCTTCCATTGTGATTGCGCAAATGTCCGTTTTTGCAATCTACTCAAGACTTTATTTCGTAGATCTTATCTCATTATTGTAGTAACATCTACGACTACTAGACACAGAGGAACAGGTATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGCGATGTTAATGGGCAAAAATTCTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGGAAGCTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGCGTATGGTCTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTACAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAAATGGAATACAACTATAACTCACATAATGTATACATCATGGCAGACAAACCAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTAAAGATGGAAGCGTTCAATTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCCAAAGATCCCAACGAAAAGAGAGATCACATGATCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAA</p> | ATTGCTTATTCCTATTGCCATTTGCTTTTATTTTGCAATACGTTTTAGCAACGCACAACACCTTATGGGTGTGCTGCTTCCATTGTGATTGCGCAAATGTCCGTTTTTGCAATCTACTCAAGACTTTATTTCGTAGATCTTATCTCATTATTGTAGTAACATCTACGACTACTAGACACAGAGGAACAGGTATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGCGATGTTAATGGGCAAAAATTCTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGGAAGCTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGCGTATGGTCTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTACAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAAATGGAATACAACTATAACTCACATAATGTATACATCATGGCAGACAAACCAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTAAAGATGGAAGCGTTCAATTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCCAAAGATCCCAACGAAAAGAGAGATCACATGATCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAA</p> | ||
| Line 249: | Line 241: | ||
</div> | </div> | ||
</div> | </div> | ||
| − | </div> | + | </div> |
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | |||
| + | <!-- Fingerprints --> | ||
| + | <div class="row"> | ||
| + | <div class="border-week"> | ||
| + | <div class="box"> | ||
| + | <div class="labtitle">Sequences for the Fingerprint-aging device</div> | ||
| + | <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew3"></span> | ||
| + | <div class="box-content"> | ||
| + | |||
| + | </div> | ||
| + | <div id="collapsiblew3" class="collapse week-content"> | ||
| + | |||
| + | <!-- when copying for next week, make sure to change labtitle, span, collapsible in last line. In each separate day change span and following div--> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | <div class="row"> | ||
| + | <div class="border-day"> | ||
| + | <div class="box"> | ||
| + | <span class="box-title"><a href="http://parts.igem.org/Part:BBa_K1590007" target="_blank">Lanosterol Synthase</a></span> | ||
| + | <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew3-1"></span> | ||
| + | <div id="collapsiblew3-1" class="collapse box-content sequences"> | ||
| + | |||
| + | <p>>BBa_K1590007 (Lanosterol_synthase)<br> | ||
| + | GCGCGAATTCGCGGCCGCTTCTAGATGACCGAAGGAACCTGCCTGCGTCGTCGTGGTGGTCCGTACAAAACCGAACCGGCGACCGACCTGGGTCGTTGGCGTCTGAACTGCGAACGTGGTCGTCAGACCTGGACCTACCTACAGGACGAACGTGCGGGTCGTGAACAGACCGGTCTGGAAGCGTACGCGCTGGGTCTGGACACCAAAAACTACTTCAAAGACCTGCCGAAAGCGCACACCGCGTTCGAAGGTGCGCTGAACGGTATGACCTTCTACGTTGGTCTACAGGCGGAAGACGGTCACTGGACCGGTGACTACGGTGGTCCGCTGTTCCTGCTGCCGGGTCTGCTGATCACCTGCCACGTTGCGCGTATCCCGCTGCCAGCTGGTTACCGAGAGGAAATTGTCCGATATCTACGATCAGTACAACTGCCGGACGGTGGTTGGGGTCTGCACATCGAAGACAAATCTACCGTTTTCGGAACCGCGCTGAACTACGTTTCTCTGCGTATCCTGGGTGTTGGTCCGGACGACCCGGACCTGGTTCGTGCGCGTAACATCCTGCACAAAAAAGGTGGTGCGGTTGCGATCCCGTCTTGGGGTAAATTCTGGCTGGCGGTTCTGAACGTTTACTCTTGGGAAGGTCTGAACACCCTGTTCCCGGAAATGTGGCTGTTCCCGGACTGGGCGCCGGCGCACCCGTCTACCCTGTGGTGCCACTGCCGTCAGGTTTACCTGCCGATGTCTTACTGCTACGCGGTTCGTCTGTCTGCGGCGGAAGACCCGCTGGTTCAGTCTCTGCGTCAGGAACTGTACGTTGAAGACTTCGCGTCTATAGATTGGCTAGCACAACGTAACAATGTTGCTCCAGATGAACTGTACACCCCGCACTCTTGGCTGCTGCGTGTTGTTTACGCGCTGCTGAACCTGTACGAACACCACCACTCTGCGCACCTGCGTCAGCGTGCGGTTCAGAAACTGTACGAACACATCGTTGCGGACGACCGTTTCACCAAATCTATCTCTATCGGTCCGATCTCTAAAACCATCAACATGCTGGTTCGTTGGTACGTTGACGGTCCGGCGTCTACCGCGTTCCAGGAACACGTTTCTCGTATCCCGGACTACCTGTGGATGGGTCTGGACGGTATGAAAATGCAGGGCACCAACGGTTCTCAGATATGGGACACCGCGTTCGCGATCCAGGCGCTGCTGGAAGCGGGTGGTCACCACCGTCCGGAGTTCTCTTCTTGCCTACAGAAAGCGCACGAGTTCCTGCGTCTGTCTCAGGTTCCAGATAATCCACCTGATTACCAGAAATACTACCGTCAGATGCGTAAAGGTGGTTTCTCTTTCTCTACCCTGGACTGCGGTTGGATCGTTTCTGACTGCACCGCGGAAGCGCTGAAAGCGGTTCTGCTGCTACAGGAAAAATGCCCGCACGTTACCGAACACATCCCGCGTGAACGTCTGTGCGACGCGGTTGCGGTTCTGCTGAACATGCGTAACCCGGACGGTGGTTTCGCGACCTACGAAACCAAACGTGGTGGTCACCTGCTGGAACTGCTGAACCCGTCTGAAGTTTTCGGTGACATCATGATCGACTACACCTATGTTGAATGCACCAGTGCGGTGATGCAGGCATTAAAATACTTCCACAAGCGCTTTCCGGAACACCGCGCAGCGGAAATCCGCGAAACGTTGACCCAAGGTCTGGAGTTCTGCCGTCGTCAGCAGCGTGCGGACGGTTCTTGGGAAGGTTCTTGGGGTGTTTGCTTCACCTACGGCACCTGGTTCGGTCTGGAAGCGTTCGCGTGCATGGGTCAGACCTACCGTGACGGAACCGCGTGCGCGGAAGTTTCTCGTGCGTGCGACTTCCTGCTGTCTCGTCAGATGGCGGACGGTGGTTGGGGTGAAGACTTCGAATCTTGCGAAGAACGTCGTTACCTACAGTCTGCGCAGTCTCAGATCCACAACACCTGCTGGGCGATGATGGGTCTGATGGCGGTTCGTCACCCGGACATCGAAGCGCAGGAACGTGGTGTTCGTTGCCTGCTGGAAAAACAGCTGCCGAACGGTGACTGGCCGCAGGAAAACATCGCGGGTGTTTTCAACAAATCTTGCGCGATCTCTTACACCTCTTACCGTAACATCTTCCCGATCTGGGCGCTGGGTCGTTTCTCTCAGCTGTACCCGGAACGTGCGCTGGCGGGTCACCCGTAATAATACTAGTAGCGGCCGCTGCAGGCGC</p> | ||
| + | |||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
</div> | </div> | ||
| Line 258: | Line 286: | ||
</div> | </div> | ||
| + | |||
| + | <!-- Nasal Mucus --> | ||
| + | <div class="row"> | ||
| + | <div class="border-week"> | ||
| + | <div class="box"> | ||
| + | <div class="labtitle">Sequences for the Nasal mucus detector</div> | ||
| + | <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew4"></span> | ||
| + | <div class="box-content"> | ||
| + | |||
| + | </div> | ||
| + | <div id="collapsiblew4" class="collapse week-content"> | ||
| + | |||
| + | <!-- when copying for next week, make sure to change labtitle, span, collapsible in last line. In each separate day change span and following div--> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | <div class="row"> | ||
| + | <div class="border-day"> | ||
| + | <div class="box"> | ||
| + | <span class="box-title"><a href="http://parts.igem.org/Part:BBa_K1590008" target="_blank">Odorant Binding Protein</a></span> | ||
| + | <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew4-1"></span> | ||
| + | <div id="collapsiblew4-1" class="collapse box-content sequences"> | ||
| + | |||
| + | <p>>BBa_K1590008 (Odorant Binding Protein 2A)<br> | ||
| + | ATGCTGTCTTTCACCCTGGAAGAAGAAGACATCACCGGCACCTGGTACGTTAAAGCGATGGTTGTTGACAAAGACTTCCCGGAAGACCGTCGTCCGCGTAAAGTTTCTCCGGTTAAAGTTACCGCGCTGGGTGGTGGTAACCTGGAAGCGACCTTCACCTTCATGCGTGAAGACCGTTGCATCCAGAAAAAAATCCTGATGCGTAAAACCGAAGAACCGGGTAAATTCTCTGCGTACGGTGGTCGTAAACTGATCTACCTGCAAGAACTGCCGGGCACCGACGACTACGTTTTCTACTGCAAAGACCAGCGTCGTGGTGGTCTGCGTTACATGGGTAAACTGGTTGGTCGTAACCCGAACACCAACCTGGAAGCGCTGGAAGAATTTAAAAAACTGGTTCAGCACAAAGGTCTGTCTGAAGAAGACATCTTCATGCCGCTGCAAACCGGTTCTTGCGTTCTGGAACAC</p> | ||
| + | |||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | |||
| + | <div class="row"> | ||
| + | <div class="border-day"> | ||
| + | <div class="box"> | ||
| + | <span class="box-title"><a href="http://parts.igem.org/Part:BBa_K1590009" target="_blank">Odorant Binding Protein - Barrel</a></span> | ||
| + | <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew4-2"></span> | ||
| + | <div id="collapsiblew4-2" class="collapse box-content sequences"> | ||
| + | |||
| + | <p>>BBa_K1590009 (Odorant Binding Protein 2A - Barrel)<br> | ||
| + | ATGCTGTCTTTCACCCTGGAAGAAGAAGACATCACCGGCACCTGGTACGTTAAAGCGATGGTTGTTGACAAAGACTTCCCGGAAGACCGTCGTCCGCGTAAAGTTTCTCCGGTTAAAGTTACCGCGCTGGGTGGTGGTAACCTGGAAGCGACCTTCACCTTCATGCGTGAAGACCGTTGCATCCAGAAAAAAATCCTGATGCGTAAAACCGAAGAACCGGGTAAATTCTCTGCGTACGGTGGTCGTAAACTGATCTACCTGCAAGAACTGCCGGGCACCGACGACTACGTTTTCTACTGCAAAGACCAGCGTCGTGGTGGTCTGCGTTACATGGGTAAACTGGTTGGTCGTAAC</p> | ||
| + | |||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | |||
| + | <div class="row"> | ||
| + | <div class="border-day"> | ||
| + | <div class="box"> | ||
| + | <span class="box-title"><a href="http://parts.igem.org/Part:BBa_K1590010" target="_blank">Odorant Binding Protein - Helix</a></span> | ||
| + | <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew4-3"></span> | ||
| + | <div id="collapsiblew4-3" class="collapse box-content sequences"> | ||
| + | |||
| + | <p>>BBa_K1590010 (Odorant Binding Protein 2A - Helix)<br> | ||
| + | CCGAACACCAACCTGGAAGCGCTGGAAGAATTTAAAAAACTGGTTCAGCACAAAGGTCTGTCTGAAGAAGACATCTTCATGCCGCTGCAAACCGGTTCTTGCGTTCTGGAACAC</p> | ||
| + | |||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | |||
| + | |||
| + | <!-- Saliva --> | ||
| + | <div class="row"> | ||
| + | <div class="border-week"> | ||
| + | <div class="box"> | ||
| + | <div class="labtitle">Sequences for the Saliva Detector</div> | ||
| + | <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew5"></span> | ||
| + | <div class="box-content"> | ||
| + | |||
| + | </div> | ||
| + | <div id="collapsiblew5" class="collapse week-content"> | ||
| + | |||
| + | <!-- when copying for next week, make sure to change labtitle, span, collapsible in last line. In each separate day change span and following div--> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | <div class="row"> | ||
| + | <div class="border-day"> | ||
| + | <div class="box"> | ||
| + | <span class="box-title"><a href="http://parts.igem.org/Part:BBa_K1590011" target="_blank">Lactoferrin Binding Protein A</a></span> | ||
| + | <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew5-1"></span> | ||
| + | <div id="collapsiblew5-1" class="collapse box-content sequences"> | ||
| + | |||
| + | <p>>BBa_K1590011 (Lactoferrin Binding Protein A)<br> | ||
| + | ATGAATAAGAAACACGGTTTTCCGCTGACTCTGACTGCCTTGGCCATTGCAACCGCTTTTCCGGCTTATGCTGCCCAAGCGGGGGGGGCGACACCTGATGCCGCGCAGACCCAATCCCTGAAAGAGATTACCGTCCGTGCCGCCAAAGTGGGACGGCGATCGAAAGAGGCGACCGGTTTGGGCAAAATCGTCAAAACGTCGGAAACGTTGAACAAAGAACAGGTACTCGGTATCCGCGACCTGACGCGCTACGATCCGGGCGTGGCGGTTGTCGAACAGGGCAACGGCGCGAGCGGCGGCTACTCGATACGCGGCGTGGATAAAAACCGTGTGGCGGTTTCGGTCGACGGTGTTGCCCAAATACAGGCGTTTACCGTGCAGGGATCGTTGAGCGGATACGGCGGACGCGGCGGCAGCGGTGCAATCAACGAAATCGAATATGAAAACATCAGCACGGTGGAAATCGACAAAGGTGCCGGTTCGTCCGATCACGGCAGCGGCGCACTCGGCGGCGCGGTCGCCTTCCGCACCAAAGAGGCGGCAGACCTGATTTCAGACGGCAAAAGTTGGGGGATACAGGCAAAAACCGCCTACGGCAGTAAAAACCGCCAATTTATGAAGTCGCTCGGCGCGGGGTTCAGCAAAGACGGTTGGGAAGGGCTGTTAATCCGAACCGAACGCCAAGGGCGGGAAACGCGCCCGCACGGCGATATTGCGGACGGGGTGGAATACGGCATAGACCGTTTGGACGCGTTCCGTCAGACATACGATATTAAACGCAAGACAAGAGAGCCATTTTTCTCAGTAGAGGGCGAGCGTGAATCCAAGCCCGTGGCAAAATTGGCGGGCTACGGGAAATATTTGAACAACCAGCTCAACCGCTGGGTAAAAGAACGTATTGAACAAAATCAGCCTTTAAGTGCTGAAGAAGAGGCGCAGGTGCGGGAGGCGCAGGCGCGCCACGAAAATCTGTCCGCCCAAGCTTACACGGGCGGCGGCAGGATATTGCCCGATCCGATGGATTACCGCAGCGGCTCTTGGCTTGCCAAGCTGGGCTACCGCTTCGGCGGCAGGCATTATGTCGGCGGCGTGTTTGAGGATACCAAACAGCGTTACGATATCCGCGATATGACGGAAAAACAGTATTACGGTACGGACGAGGCGGAAAAGTTTAGAGACAAGAGCGGGGTGTACGACGGCGACGATTTCCGCGACGGCTTGTATTTTGTGCCGAATATAGAAGAGTGGAAGGGCGATAAAAATTTGGTCAGGGGCATAGGTTTGAAATATTCCCGCACCAAATTTATTGACGAACATCACCGCCGCCGCCGTATGGGTTTGCTGTATCGTTATGAAAACGAAGCGTATTCTGACAATTGGGCGGATAAGGCGGTGTTGTCGTTTGACAAACAGGGCGTGGCAACCGATAACAACACGCTGAAGCTGAATTGCGCCGTGTATCCTGCTGTGGACAAATCCTGCCGCGCGTCGGCGGACAAACCGTATTCCTACGACAGCAGCGACCGTTTCCACTACCGCGAACAGCACAATGTTTTGAATGCCTCGTTTGAGAAATCGCTGAAAAACAAATGGACGAAACACCATCTGACTTTGGGCTTCGGTTACGATGCTTCCAAAGCGATTTCCCGCCCCGAACAGCTTTCCCACAATGCGGCAAGGATTTCGGAATCCACGGGATTCGATGAAAACAATCAAGATAAGTATCTTTTGGGTAAGCCCGAAGTCGTCGAAGGGTCGGTCTGCGGCTACATCGAAACCCTGCGTTCCCGCAAATGCGTGCCAAGAAAAATCAACGGCAGCAATATCCATATTTCTTTGAACGACCGTTTTTCAATCGGCAAATATTTCGATTTCAGCTTGGGCGGCAGGTACGACCGGAAAAACTTCACCACGTCGGAAGAACTCGTCCGCAGCGGGCGGTATGTTGACCGTTCGTGGAACAGCGGCATCTTGTTCAAACCGAACCGGCATTTTTCCGTGTCTTACCGTGCCTCCAGCGGCTTCAGAACGCCCTCATTCCAAGAACTTTTCGGGATAGACATTTATCACGATTATCCGAAAGGCTGGCAGCGTCCCGCCCTGAAATCGGAAAAGGCAGCCAACCGGGAAATCGGTTTGCAGTGGAAGGGCGATTTCGGCTTTTTGGAAATCAGCAGCTTCCGCAACCGTTATACCGATATGATTGCCGTTGCCGATCACAAAACCAAATTGCCGAATCAGGCAGGACAATTGACAGAGATTGATATACGCGATTATTACAATGCCCAAAATATGTCGCTTCAAGGCGTTAATATATTGGGAAAAATCGACTGGAACGGCGTGTATGGCAAACTGCCCGAAGGTTTGTACACCACATTGGCGTACAACCGCATCAAACCGAAATCGGTATCCAACCGGCCGGGACTGTCCCTCCGCAGCTATGCTTTGGATGCGGTACAGCCGTCGCGTTATGTTTTGGGGTTCGGATACGACCAGCCTGAGGGGAAATGGGGCGCAAACATTATGCTGACCTATTCCAAAGGGAAAAACCCTGACGAGCTTGCTTATCTGGCAGGCGATCAAAAACGATATTCGACAAAAAGAGCGTCGTCTTCTTGGTCGACGGCAGACGTTTCCGCCTATCTGAATCTGAAAAAACGGCTGACCTTGAGGGCGGCTATCTACAATATCGGCAACTACCGCTACGTTACTTGGGAATCCTTGCGCCAGACTGCGGAAAGCACGGCAAACCGGCACGGCGGCGACAGCAACTATGGAAGGTATGCCGCACCGGGCAGGAACTTCAGTCTCGCGCTCGAAATGAAGTTTTAA</p> | ||
| + | |||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | |||
| + | |||
| + | <!-- Saliva --> | ||
| + | <div class="row"> | ||
| + | <div class="border-week"> | ||
| + | <div class="box"> | ||
| + | <div class="labtitle">Sequences for the Saliva Detector</div> | ||
| + | <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew6"></span> | ||
| + | <div class="box-content"> | ||
| + | |||
| + | </div> | ||
| + | <div id="collapsiblew6" class="collapse week-content"> | ||
| + | |||
| + | <!-- when copying for next week, make sure to change labtitle, span, collapsible in last line. In each separate day change span and following div--> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | <div class="row"> | ||
| + | <div class="border-day"> | ||
| + | <div class="box"> | ||
| + | <span class="box-title"><a href="http://parts.igem.org/Part:BBa_K1590012" target="_blank">PotD (Spermidine Binding Protein)</a></span> | ||
| + | <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew6-1"></span> | ||
| + | <div id="collapsiblew6-1" class="collapse box-content sequences"> | ||
| + | |||
| + | <p>>BBa_K1590012 (PotD)<br> | ||
| + | ATGAAAAAATGGTCACGCCACCTGCTCGCGGCGGGTGCTCTGGCACTGGGCATGAGCGCCGCTCACGCCGATGACAACAACACGCTGTATTTCTACAACTGGACCGAGTACGTGCCGCCAGGACTGCTTGAACAGTTCACCAAAGAAACCGGTATTAAGGTTATCTATTCGACTTACGAGTCGAACGAAACCATGTACGCCAAGCTGAAAACATACAAAGACGGTGCCTATGATCTGGTGGTTCCTTCAACCTATTACGTTGATAAAATGCGTAAAGAAGGGATGATCCAGAAGATCGACAAGTCGAAGTTAACAAACTTCAGCAATCTCGATCCAGACATGCTCAACAAGCCTTTTGACCCGAATAACGACTACTCCATTCCGTATATCTGGGGTGCGACGGCGATTGGTGTTAACGGTGATGCGGTGGATCCGAAATCTGTCACCAGCTGGGCCGATCTGTGGAAGCCAGAGTACAAAGGCAGCCTGCTGTTGACCGACGATGCCCGTGAAGTGTTCCAGATGGCGCTGCGTAAGCTGGGCTACTCCGGTAACACCACCGATCCGAAAGAGATTGAAGCTGCATATAACGAGCTGAAAAAACTGATGCCAAACGTGGCAGCCTTTAACTCCGATAACCCGGCGAACCCGTACATGGAAGGCGAAGTTAACCTCGGCATGATCTGGAACGGTTCTGCTTTCGTTGCACGCCAGGCGGGTACGCCAATTGACGTGGTGTGGCCGAAAGAAGGCGGCATTTTCTGGATGGACAGCCTGGCGATCCCGGCAAATGCCAAAAACAAAGAAGGCGCGCTGAAATTGATCAACTTCCTGCTGCGCCCGGATGTGGCAAAACAGGTTGCTGAAACTATCGGTTATCCAACGCCAAACCTTGCGGCGCGTAAGCTGTTAAGTCCAGAAGTGGCGAACGATAAAACACTCTACCCGGATGCTGAAACCATTAAAAATGGCGAATGGCAGAATGACGTTGGCGCAGCCAGCAGCATTTATGAAGAGTATTATCAGAAGCTGAAAGCAGGACGTTAA</p> | ||
| + | |||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | |||
| + | <div class="row"> | ||
| + | <div class="border-day"> | ||
| + | <div class="box"> | ||
| + | <span class="box-title"><a href="http://parts.igem.org/Part:BBa_K1590013" target="_blank">Spermine Binding Protein</a></span> | ||
| + | <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew6-2"></span> | ||
| + | <div id="collapsiblew6-2" class="collapse box-content sequences"> | ||
| + | |||
| + | <p>>BBa_K1590013 (Spermine Binding Protein)<br> | ||
| + | ATGCTGCTGCTGCTGACCCTGGCGTTCCTGGCGTCTCCGACCTGCCGTGCGCAGAACGTTCTGGGTAACGCGGCGGGTAAATACTTCTACGTTCAGGGTGAAGACCAGGGTCAGCTGAAAGGTATGCGTATCTTCCTGTCTGTTTTCAAATTCATCAAAGGTTTCCAGCTCCAGTTCGGTTCTAACTGGACCGACGTTTACGGCACCCGTTCTGACAACTTCATCGACTTCCTGCTGGAAGACGGTGAACACGTTATCAAAGTTGAAGGTTCTGCGGTTATCTGCCTGACCTCTCTGACCTTCACCACCAACAAAGGTCGTGTTGCGACCTTCGGTGTTCGTCGTGGTCGTTACTTCTCTGACACCGGTGGTTCTGACAAACACCTGGTTACCGTTAACGGTATGCACGCGCCGGGTCTGTGCGTTCGTGGTATCGGTTTCAAATGGGGTAACATCAACGCGAACGGTAACGACCACTACAACAACAAAGAAGACAAAGCGGACAACAAAGACGCGGACAACAAAGACGCTGACAATAAAGACGATGGCGATGAGGATGATGATGGAAACGATGACGATGATCAGAAGGACGAATCTTAA</p> | ||
| + | |||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | |||
| + | |||
| + | <!-- Vectors --> | ||
| + | <div class="row"> | ||
| + | <div class="border-week"> | ||
| + | <div class="box"> | ||
| + | <div class="labtitle">Vectors used throughout the project</div> | ||
| + | <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew7"></span> | ||
| + | <div class="box-content"> | ||
| + | |||
| + | </div> | ||
| + | <div id="collapsiblew7" class="collapse week-content"> | ||
| + | |||
| + | <!-- when copying for next week, make sure to change labtitle, span, collapsible in last line. In each separate day change span and following div--> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | <div class="row"> | ||
| + | <div class="border-day"> | ||
| + | <div class="box"> | ||
| + | <span class="box-title"><a href="http://parts.igem.org/Part:pSB1C3" target="_blank">pSB1C3</a></span> | ||
| + | <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew7-1"></span> | ||
| + | <div id="collapsiblew7-1" class="collapse box-content sequences"> | ||
| + | |||
| + | <p></p> | ||
| + | |||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | |||
| + | <div class="row"> | ||
| + | <div class="border-day"> | ||
| + | <div class="box"> | ||
| + | <span class="box-title">pQE-80L</span> <!--Title of collapsible box--> | ||
| + | <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew7-2"></span> | ||
| + | <div id="collapsiblew7-2" class="collapse box-content sequences"> | ||
| + | |||
| + | <p></p> | ||
| + | |||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | |||
| + | <div class="row"> | ||
| + | <div class="border-day"> | ||
| + | <div class="box"> | ||
| + | <span class="box-title">pUT18</span> <!--Title of collapsible box--> | ||
| + | <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew7-3"></span> | ||
| + | <div id="collapsiblew7-3" class="collapse box-content sequences"> | ||
| + | |||
| + | <p></p> | ||
| + | |||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | |||
| + | <div class="row"> | ||
| + | <div class="border-day"> | ||
| + | <div class="box"> | ||
| + | <span class="box-title">pT25</span> <!--Title of collapsible box--> | ||
| + | <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew7-4"></span> | ||
| + | <div id="collapsiblew7-4" class="collapse box-content sequences"> | ||
| + | |||
| + | <p></p> | ||
| + | |||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | |||
| + | <div class="row"> | ||
| + | <div class="border-day"> | ||
| + | <div class="box"> | ||
| + | <span class="box-title">pUniprom</span> <!--Title of collapsible box--> | ||
| + | <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#collapsiblew7-5"></span> | ||
| + | <div id="collapsiblew7-5" class="collapse box-content sequences"> | ||
| + | |||
| + | <p></p> | ||
| + | |||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | |||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
Revision as of 18:23, 20 August 2015
Sequences and Parts
Detecting Blood
The biobricks in this section are designed for the detection of blood. All sequences are synthetically optimised for expression in our chassis.
>BBa_K1590000 (Haemoglobin A)
ATGGTTCTGTCTCCGGCGGACAAAACCAACGTTAAAGCGGCGTGGGGTAAAGTTGGTGCGCACGCGGGTGAATACGGTGCGGAAGCGCTGGAACGTATGTTCCTGTCTTTCCCGACCACCAAAACCTACTTCCCGCACTTCGACCTGTCTCACGGTTCTGCGCAGGTTAAAGGTCACGGTAAAAAAGTTGCGGACGCGCTGACCAACGCGGTTGCGCACGTTGACGACATGCCGAACGCGCTGTCTGCGCTGTCTGACCTGCACGCGCACAAACTGCGTGTTGACCCGGTTAACTTCAAACTGCTGTCTCACTGCCTGCTGGTTACCCTGGCGGCGCACCTGCCGGCGGAGTTCACCCCGGCGGTTCACGCGTCTCTGGACAAATTCCTGGCGTCTGTTTCTACCGTTCTGACCTCTAAATACCGTTAA
>BBa_K1590001 (Haemoglobin B)
ATGGTTCACCTGACCCCGGAAGAAAAATCTGCGGTTACCGCGCTGTGGGGTAAAGTTAACGTTGACGAAGTTGGTGGTGAAGCGCTGGGTCGTCTGCTGGTTGTTTACCCGTGGACCCAGCGTTTCTTCGAATCTTTCGGTGACCTGTCTACCCCGGACGCGGTTATGGGTAACCCGAAAGTTAAAGCGCACGGTAAAAAAGTTCTGGGTGCGTTCTCTGACGGTCTGGCGCACCTGGACAACCTGAAAGGCACCTTCGCGACCCTGTCTGAACTGCACTGCGACAAACTGCACGTTGACCCGGAAAACTTCCGTCTGCTGGGTAACGTTCTGGTTTGCGTTCTGGCGCACCACTTCGGTAAAGAGTTCACCCCGCCGGTTCAGGCGGCGTACCAGAAAGTTGTTGCGGGTGTTGCGAACGCGCTGGCGCACAAATACCACTAA
>BBa_K1590002 (Haptoglobin)
ATGTCTGCGCTGGGTGCGGTTATCGCGCTGCTGCTGTGGGGTCAGCTGTTCGCGGTTGACTCTGGTAACGACGTTACCGACATCGCGGACGACGGTTGCCCGAAACCGCCGGAAATCGCGCACGGTTACGTTGAACACTCTGTTCGTTACCAGTGCAAAAACTACTACAAACTGCGTACCGAAGGTGACGGTGTTTACACCCTGAACGACAAAAAACAGTGGATCAACAAAGCAGTAGGCGACAAACTGCCGGAATGCGAAGCCGATGACGGCTGTCCGAAACCACCGGAGATCGCTCATGGCTACGTCGAACACTCGGTGCGTTATCAGTGCAAAAACTACTACAAACTGCGCACGGAAGGAGACGGGGTGTACACTCTTAATAACGAGAAACAGTGGATTAACAAAGCGGTTGGCGATAAGCTGCCGGAATGTGAGGCAGTCTGCGGTAAACCGAAAAACCCGGCGAACCCGGTTCAGCGTATCTTGGGCGGGCACTTAGATGCGAAAGGTTCATTCCCGTGGCAGGCCAAAATGGTTTCCCACCACAATCTCACGACAGGGGCGACCCTGATCAACGAGCAGTGGTTGCTGACCACTGCTAAAAATCTGTTTCTGAACCATAGCGAAAATGCGACGGCGAAAGACATCGCGCCGACCCTGACCCTGTACGTTGGTAAAAAACAGCTGGTTGAAATCGAAAAAGTTGTTCTGCACCCGAACTACTCTCAGGTTGACATCGGTCTGATCAAACTGAAACAGAAAGTTTCTGTTAACGAACGTGTTATGCCGATCTGCCTGCCGTCTAAAGACTACGCGGAAGTTGGTCGTGTTGGTTACGTTTCTGGTTGGGGTCGTAACGCGAACTTCAAATTCACCGACCACCTGAAATACGTTATGCTGCCGGTTGCGGACCAGGACCAGTGCATCCGTCACTACGAAGGTTCTACCGTTCCGGAAAAAAAAACCCCGAAATCTCCGGTTGGTGTTCAGCCGATCCTGAACGAACACACCTTCTGCGCGGGTATGTCTAAATACCAGGAAGACACCTGCTACGGTGACGCGGGTTCTGCGTTCGCGGTTCACGACCTGGAAGAAGACACCTGGTACGCGACCGGTATCCTGTCTTTCGACAAATCTTGCGCGGTTGCGGAATACGGTGTTTACGTTAAAGTTACCTCTATCCAGGACTGGGTTCAGAAAACCATCGCGGAAAACTAA
Detecting Chromate
This set of biobricks is based on an operon of ochrobactrum tritici, conferring resistance against Cr(VI). The Beijing institute of Biotechnology already submitted a biobrick based on this operon in 2013, BBa_K1058008 (See Figure 1). We used this brick, broke it down to its constituent parts, and reassembled it in a different way (See Figure 2).
by BIT2013.
These 2 biobricks are transformed into one cell in order to rebuild a chromate sensor.
Recommended reading:
- Branco R et al. The Chromate-Inducible chrBACF Operon from the Transposable Element TnOtChr Confers Resistance to Chromium(VI) and Superoxide. Journal of Bacteriology. 2008;190(21):6996-7003.
- Branco R et al. Highly Sensitive, Highly Specific Whole-Cell Bioreporters for the Detection of Chromate in Environmental Samples. PloS one. 2013;8(1):e54005.
>BBa_K1058008
ATTGCTTATTCCTATTGCCATTTGCTTTTATTTTGCAATACGTTTTAGCAACGCACAACACCTTATGGGTGTGCTGCTTCCATTGTGATTGCGCAAATGTCCGTTTTTGCAATCTACTCAAGACTTTATTTCGTAGATCTTATCTCATTATTGTAGTAACATCTACGACATGAATTTGCTTTCGCCTATCCTTTCCTTGCCTACTGAAAACGCGACCGTCCGGCAACGGACGTGGCGTGCCCTCAAAGCCTCCGGCGCTGCGGTGCTGCGCGACGGCGTGTACCTGATGCCCGACCGCGACGAATGCCGCGCGGTGCTGGATAACTTGGCCTCCGATGTGCGTGAAGGCGGTGGGGTCGCTCATGTGCTGCGTATGGAAGACCCTGAAGGGGTCAACTTCGTCGCATTATTCGACCGCAGCAACGATTTTGCTGCCTTGCTGGTCGATGTCCATCATCTCAGGCAGACGCTGACATTGGATACCGTGCAAGACGTGCTGCGGCAGGTGCGTAAGCTTCGCAAATCCTTTACTACGCTGGTTGAAATCGACTTCTACCCCGGCGAGGCTCAGCGTCAAGCGGACAGTGCGTTGTGTGAACTGGAACAAGCTTGCGCCCGCACGCTGTCGCCGGACGAGCCGCACGCTGTAGAGGGGACTATTACCCGCTTGGATCGTTTGGATTACCAGGCCCGTACCTGGGCCACTCGCGCACGTCCTTGGGTTGATCGGCTCGCCAGCGCATGGCTGATCCGGCGCTTCATCGACCCGCAGGCGCGCATCCTTTGGTTGGCAACCCCTGCGGACTGCCCGCCGGATGCGTTGGGCTTCGACTTCGATGGCGCGACGTTCAGCCATGTCGGCAGCCGTGTCACGTTCGAGGTCCTGGCGGCGAGCTTTGGGCTGGAACAGCCCGCCATCACAAGGATTGGCCTTGTGGTGCATTACCTCGACGTGGGCGGCATCCAGCCGCCAGAGGCCACTGGTATCGAAAGCGTACTGGCCGGTTTGCGGGAAACGGTTGACCACGACGATCAACTATTGGCCATCGCCTCCACTGTGTTCGATGGCTTACTGGCCAGCTTTGAAAAAGGGACCCTCACCGTATGAGGATCCGAAGGAGATATACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTA
>BBa_K1590003 (pChr)
ATTGCTTATTCCTATTGCCATTTGCTTTTATTTTGCAATACGTTTTAGCAACGCACAACACCTTATGGGTGTGCTGCTTCCATTGTGATTGCGCAAATGTCCGTTTTTGCAATCTACTCAAGACTTTATTTCGTAGATCTTATCTCATTATTGTAGTAACATCTACGAC
>BBa_K1590004 (ChrB)
ATGAATTTGCTTTCGCCTATCCTTTCCTTGCCTACTGAAAACGCGACCGTCCGGCAACGGACGTGGCGTGCCCTCAAAGCCTCCGGCGCTGCGGTGCTGCGCGACGGCGTGTACCTGATGCCCGACCGCGACGAATGCCGCGCGGTGCTGGATAACTTGGCCTCCGATGTGCGTGAAGGCGGTGGGGTCGCTCATGTGCTGCGTATGGAAGACCCTGAAGGGGTCAACTTCGTCGCATTATTCGACCGCAGCAACGATTTTGCTGCCTTGCTGGTCGATGTCCATCATCTCAGGCAGACGCTGACATTGGATACCGTGCAAGACGTGCTGCGGCAGGTGCGTAAGCTTCGCAAATCCTTTACTACGCTGGTTGAAATCGACTTCTACCCCGGCGAGGCTCAGCGTCAAGCGGACAGTGCGTTGTGTGAACTGGAACAAGCTTGCGCCCGCACGCTGTCGCCGGACGAGCCGCACGCTGTAGAGGGGACTATTACCCGCTTGGATCGTTTGGATTACCAGGCCCGTACCTGGGCCACTCGCGCACGTCCTTGGGTTGATCGGCTCGCCAGCGCATGGCTGATCCGGCGCTTCATCGACCCGCAGGCGCGCATCCTTTGGTTGGCAACCCCTGCGGACTGCCCGCCGGATGCGTTGGGCTTCGACTTCGATGGCGCGACGTTCAGCCATGTCGGCAGCCGTGTCACGTTCGAGGTCCTGGCGGCGAGCTTTGGGCTGGAACAGCCCGCCATCACAAGGATTGGCCTTGTGGTGCATTACCTCGACGTGGGCGGCATCCAGCCGCCAGAGGCCACTGGTATCGAAAGCGTACTGGCCGGTTTGCGGGAAACGGTTGACCACGACGATCAACTATTGGCCATCGCCTCCACTGTGTTCGATGGCTTACTGGCCAGCTTTGAAAAAGGGACCCTCACCGTATGA
>BBa_K1590005 (Partially optimised ChrB)
ATGAACCTGCTGTCTCCGATCCTGTCTCTGCCGACCGAAAACGCGACCGTCCGGCAACGGACGTGGCGTGCCCTCAAAGCCTCCGGCGCTGCGGTGCTGCGCGACGGCGTGTACCTGATGCCCGACCGCGACGAATGCCGCGCGGTGCTGGATAACTTGGCCTCCGATGTGCGTGAAGGCGGTGGGGTCGCTCATGTGCTGCGTATGGAAGACCCTGAAGGGGTCAACTTCGTCGCATTATTCGACCGCAGCAACGATTTTGCTGCCTTGCTGGTCGATGTCCATCATCTCAGGCAGACGCTGACATTGGATACCGTGCAAGACGTGCTGCGGCAGGTGCGTAAGCTTCGCAAATCCTTTACTACGCTGGTTGAAATCGACTTCTACCCCGGCGAGGCTCAGCGTCAAGCGGACAGTGCGTTGTGTGAACTGGAACAAGCTTGCGCCCGCACGCTGTCGCCGGACGAGCCGCACGCTGTAGAGGGGACTATTACCCGCTTGGATCGTTTGGATTACCAGGCCCGTACCTGGGCCACTCGCGCACGTCCTTGGGTTGATCGGCTCGCCAGCGCATGGCTGATCCGGCGCTTCATCGACCCGCAGGCGCGCATCCTTTGGTTGGCAACCCCTGCGGACTGCCCGCCGGATGCGTTGGGCTTCGACTTCGATGGCGCGACGTTCAGCCATGTCGGCAGCCGTGTCACGTTCGAGGTCCTGGCGGCGAGCTTTGGGCTGGAACAGCCCGCCATCACAAGGATTGGCCTTGTGGTGCATTACCTCGACGTGGGCGGCATCCAGCCGCCAGAGGCCACTGGTATCGAAAGCGTACTGGCCGGTTTGCGGGAAACGGTTGACCACGACGATCAACTATTGGCCATCGCCTCCACTGTGTTCGATGGCTTACTGGCCAGCTTTGAAAAAGGGACCCTCACCGTATGA
>GFPmut2
ATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGCGATGTTAATGGGCAAAAATTCTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGGAAGCTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGCGTATGGTCTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTACAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAAATGGAATACAACTATAACTCACATAATGTATACATCATGGCAGACAAACCAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTAAAGATGGAAGCGTTCAATTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCCAAAGATCCCAACGAAAAGAGAGATCACATGATCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAA
>BBa_K1590006 (pChrGFP)
ATTGCTTATTCCTATTGCCATTTGCTTTTATTTTGCAATACGTTTTAGCAACGCACAACACCTTATGGGTGTGCTGCTTCCATTGTGATTGCGCAAATGTCCGTTTTTGCAATCTACTCAAGACTTTATTTCGTAGATCTTATCTCATTATTGTAGTAACATCTACGACTACTAGACACAGAGGAACAGGTATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGCGATGTTAATGGGCAAAAATTCTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGGAAGCTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGCGTATGGTCTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTACAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAAATGGAATACAACTATAACTCACATAATGTATACATCATGGCAGACAAACCAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTAAAGATGGAAGCGTTCAATTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCCAAAGATCCCAACGAAAAGAGAGATCACATGATCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAA
>BBa_K1590007 (Lanosterol_synthase)
GCGCGAATTCGCGGCCGCTTCTAGATGACCGAAGGAACCTGCCTGCGTCGTCGTGGTGGTCCGTACAAAACCGAACCGGCGACCGACCTGGGTCGTTGGCGTCTGAACTGCGAACGTGGTCGTCAGACCTGGACCTACCTACAGGACGAACGTGCGGGTCGTGAACAGACCGGTCTGGAAGCGTACGCGCTGGGTCTGGACACCAAAAACTACTTCAAAGACCTGCCGAAAGCGCACACCGCGTTCGAAGGTGCGCTGAACGGTATGACCTTCTACGTTGGTCTACAGGCGGAAGACGGTCACTGGACCGGTGACTACGGTGGTCCGCTGTTCCTGCTGCCGGGTCTGCTGATCACCTGCCACGTTGCGCGTATCCCGCTGCCAGCTGGTTACCGAGAGGAAATTGTCCGATATCTACGATCAGTACAACTGCCGGACGGTGGTTGGGGTCTGCACATCGAAGACAAATCTACCGTTTTCGGAACCGCGCTGAACTACGTTTCTCTGCGTATCCTGGGTGTTGGTCCGGACGACCCGGACCTGGTTCGTGCGCGTAACATCCTGCACAAAAAAGGTGGTGCGGTTGCGATCCCGTCTTGGGGTAAATTCTGGCTGGCGGTTCTGAACGTTTACTCTTGGGAAGGTCTGAACACCCTGTTCCCGGAAATGTGGCTGTTCCCGGACTGGGCGCCGGCGCACCCGTCTACCCTGTGGTGCCACTGCCGTCAGGTTTACCTGCCGATGTCTTACTGCTACGCGGTTCGTCTGTCTGCGGCGGAAGACCCGCTGGTTCAGTCTCTGCGTCAGGAACTGTACGTTGAAGACTTCGCGTCTATAGATTGGCTAGCACAACGTAACAATGTTGCTCCAGATGAACTGTACACCCCGCACTCTTGGCTGCTGCGTGTTGTTTACGCGCTGCTGAACCTGTACGAACACCACCACTCTGCGCACCTGCGTCAGCGTGCGGTTCAGAAACTGTACGAACACATCGTTGCGGACGACCGTTTCACCAAATCTATCTCTATCGGTCCGATCTCTAAAACCATCAACATGCTGGTTCGTTGGTACGTTGACGGTCCGGCGTCTACCGCGTTCCAGGAACACGTTTCTCGTATCCCGGACTACCTGTGGATGGGTCTGGACGGTATGAAAATGCAGGGCACCAACGGTTCTCAGATATGGGACACCGCGTTCGCGATCCAGGCGCTGCTGGAAGCGGGTGGTCACCACCGTCCGGAGTTCTCTTCTTGCCTACAGAAAGCGCACGAGTTCCTGCGTCTGTCTCAGGTTCCAGATAATCCACCTGATTACCAGAAATACTACCGTCAGATGCGTAAAGGTGGTTTCTCTTTCTCTACCCTGGACTGCGGTTGGATCGTTTCTGACTGCACCGCGGAAGCGCTGAAAGCGGTTCTGCTGCTACAGGAAAAATGCCCGCACGTTACCGAACACATCCCGCGTGAACGTCTGTGCGACGCGGTTGCGGTTCTGCTGAACATGCGTAACCCGGACGGTGGTTTCGCGACCTACGAAACCAAACGTGGTGGTCACCTGCTGGAACTGCTGAACCCGTCTGAAGTTTTCGGTGACATCATGATCGACTACACCTATGTTGAATGCACCAGTGCGGTGATGCAGGCATTAAAATACTTCCACAAGCGCTTTCCGGAACACCGCGCAGCGGAAATCCGCGAAACGTTGACCCAAGGTCTGGAGTTCTGCCGTCGTCAGCAGCGTGCGGACGGTTCTTGGGAAGGTTCTTGGGGTGTTTGCTTCACCTACGGCACCTGGTTCGGTCTGGAAGCGTTCGCGTGCATGGGTCAGACCTACCGTGACGGAACCGCGTGCGCGGAAGTTTCTCGTGCGTGCGACTTCCTGCTGTCTCGTCAGATGGCGGACGGTGGTTGGGGTGAAGACTTCGAATCTTGCGAAGAACGTCGTTACCTACAGTCTGCGCAGTCTCAGATCCACAACACCTGCTGGGCGATGATGGGTCTGATGGCGGTTCGTCACCCGGACATCGAAGCGCAGGAACGTGGTGTTCGTTGCCTGCTGGAAAAACAGCTGCCGAACGGTGACTGGCCGCAGGAAAACATCGCGGGTGTTTTCAACAAATCTTGCGCGATCTCTTACACCTCTTACCGTAACATCTTCCCGATCTGGGCGCTGGGTCGTTTCTCTCAGCTGTACCCGGAACGTGCGCTGGCGGGTCACCCGTAATAATACTAGTAGCGGCCGCTGCAGGCGC
>BBa_K1590008 (Odorant Binding Protein 2A)
ATGCTGTCTTTCACCCTGGAAGAAGAAGACATCACCGGCACCTGGTACGTTAAAGCGATGGTTGTTGACAAAGACTTCCCGGAAGACCGTCGTCCGCGTAAAGTTTCTCCGGTTAAAGTTACCGCGCTGGGTGGTGGTAACCTGGAAGCGACCTTCACCTTCATGCGTGAAGACCGTTGCATCCAGAAAAAAATCCTGATGCGTAAAACCGAAGAACCGGGTAAATTCTCTGCGTACGGTGGTCGTAAACTGATCTACCTGCAAGAACTGCCGGGCACCGACGACTACGTTTTCTACTGCAAAGACCAGCGTCGTGGTGGTCTGCGTTACATGGGTAAACTGGTTGGTCGTAACCCGAACACCAACCTGGAAGCGCTGGAAGAATTTAAAAAACTGGTTCAGCACAAAGGTCTGTCTGAAGAAGACATCTTCATGCCGCTGCAAACCGGTTCTTGCGTTCTGGAACAC
>BBa_K1590009 (Odorant Binding Protein 2A - Barrel)
ATGCTGTCTTTCACCCTGGAAGAAGAAGACATCACCGGCACCTGGTACGTTAAAGCGATGGTTGTTGACAAAGACTTCCCGGAAGACCGTCGTCCGCGTAAAGTTTCTCCGGTTAAAGTTACCGCGCTGGGTGGTGGTAACCTGGAAGCGACCTTCACCTTCATGCGTGAAGACCGTTGCATCCAGAAAAAAATCCTGATGCGTAAAACCGAAGAACCGGGTAAATTCTCTGCGTACGGTGGTCGTAAACTGATCTACCTGCAAGAACTGCCGGGCACCGACGACTACGTTTTCTACTGCAAAGACCAGCGTCGTGGTGGTCTGCGTTACATGGGTAAACTGGTTGGTCGTAAC
>BBa_K1590010 (Odorant Binding Protein 2A - Helix)
CCGAACACCAACCTGGAAGCGCTGGAAGAATTTAAAAAACTGGTTCAGCACAAAGGTCTGTCTGAAGAAGACATCTTCATGCCGCTGCAAACCGGTTCTTGCGTTCTGGAACAC
>BBa_K1590011 (Lactoferrin Binding Protein A)
ATGAATAAGAAACACGGTTTTCCGCTGACTCTGACTGCCTTGGCCATTGCAACCGCTTTTCCGGCTTATGCTGCCCAAGCGGGGGGGGCGACACCTGATGCCGCGCAGACCCAATCCCTGAAAGAGATTACCGTCCGTGCCGCCAAAGTGGGACGGCGATCGAAAGAGGCGACCGGTTTGGGCAAAATCGTCAAAACGTCGGAAACGTTGAACAAAGAACAGGTACTCGGTATCCGCGACCTGACGCGCTACGATCCGGGCGTGGCGGTTGTCGAACAGGGCAACGGCGCGAGCGGCGGCTACTCGATACGCGGCGTGGATAAAAACCGTGTGGCGGTTTCGGTCGACGGTGTTGCCCAAATACAGGCGTTTACCGTGCAGGGATCGTTGAGCGGATACGGCGGACGCGGCGGCAGCGGTGCAATCAACGAAATCGAATATGAAAACATCAGCACGGTGGAAATCGACAAAGGTGCCGGTTCGTCCGATCACGGCAGCGGCGCACTCGGCGGCGCGGTCGCCTTCCGCACCAAAGAGGCGGCAGACCTGATTTCAGACGGCAAAAGTTGGGGGATACAGGCAAAAACCGCCTACGGCAGTAAAAACCGCCAATTTATGAAGTCGCTCGGCGCGGGGTTCAGCAAAGACGGTTGGGAAGGGCTGTTAATCCGAACCGAACGCCAAGGGCGGGAAACGCGCCCGCACGGCGATATTGCGGACGGGGTGGAATACGGCATAGACCGTTTGGACGCGTTCCGTCAGACATACGATATTAAACGCAAGACAAGAGAGCCATTTTTCTCAGTAGAGGGCGAGCGTGAATCCAAGCCCGTGGCAAAATTGGCGGGCTACGGGAAATATTTGAACAACCAGCTCAACCGCTGGGTAAAAGAACGTATTGAACAAAATCAGCCTTTAAGTGCTGAAGAAGAGGCGCAGGTGCGGGAGGCGCAGGCGCGCCACGAAAATCTGTCCGCCCAAGCTTACACGGGCGGCGGCAGGATATTGCCCGATCCGATGGATTACCGCAGCGGCTCTTGGCTTGCCAAGCTGGGCTACCGCTTCGGCGGCAGGCATTATGTCGGCGGCGTGTTTGAGGATACCAAACAGCGTTACGATATCCGCGATATGACGGAAAAACAGTATTACGGTACGGACGAGGCGGAAAAGTTTAGAGACAAGAGCGGGGTGTACGACGGCGACGATTTCCGCGACGGCTTGTATTTTGTGCCGAATATAGAAGAGTGGAAGGGCGATAAAAATTTGGTCAGGGGCATAGGTTTGAAATATTCCCGCACCAAATTTATTGACGAACATCACCGCCGCCGCCGTATGGGTTTGCTGTATCGTTATGAAAACGAAGCGTATTCTGACAATTGGGCGGATAAGGCGGTGTTGTCGTTTGACAAACAGGGCGTGGCAACCGATAACAACACGCTGAAGCTGAATTGCGCCGTGTATCCTGCTGTGGACAAATCCTGCCGCGCGTCGGCGGACAAACCGTATTCCTACGACAGCAGCGACCGTTTCCACTACCGCGAACAGCACAATGTTTTGAATGCCTCGTTTGAGAAATCGCTGAAAAACAAATGGACGAAACACCATCTGACTTTGGGCTTCGGTTACGATGCTTCCAAAGCGATTTCCCGCCCCGAACAGCTTTCCCACAATGCGGCAAGGATTTCGGAATCCACGGGATTCGATGAAAACAATCAAGATAAGTATCTTTTGGGTAAGCCCGAAGTCGTCGAAGGGTCGGTCTGCGGCTACATCGAAACCCTGCGTTCCCGCAAATGCGTGCCAAGAAAAATCAACGGCAGCAATATCCATATTTCTTTGAACGACCGTTTTTCAATCGGCAAATATTTCGATTTCAGCTTGGGCGGCAGGTACGACCGGAAAAACTTCACCACGTCGGAAGAACTCGTCCGCAGCGGGCGGTATGTTGACCGTTCGTGGAACAGCGGCATCTTGTTCAAACCGAACCGGCATTTTTCCGTGTCTTACCGTGCCTCCAGCGGCTTCAGAACGCCCTCATTCCAAGAACTTTTCGGGATAGACATTTATCACGATTATCCGAAAGGCTGGCAGCGTCCCGCCCTGAAATCGGAAAAGGCAGCCAACCGGGAAATCGGTTTGCAGTGGAAGGGCGATTTCGGCTTTTTGGAAATCAGCAGCTTCCGCAACCGTTATACCGATATGATTGCCGTTGCCGATCACAAAACCAAATTGCCGAATCAGGCAGGACAATTGACAGAGATTGATATACGCGATTATTACAATGCCCAAAATATGTCGCTTCAAGGCGTTAATATATTGGGAAAAATCGACTGGAACGGCGTGTATGGCAAACTGCCCGAAGGTTTGTACACCACATTGGCGTACAACCGCATCAAACCGAAATCGGTATCCAACCGGCCGGGACTGTCCCTCCGCAGCTATGCTTTGGATGCGGTACAGCCGTCGCGTTATGTTTTGGGGTTCGGATACGACCAGCCTGAGGGGAAATGGGGCGCAAACATTATGCTGACCTATTCCAAAGGGAAAAACCCTGACGAGCTTGCTTATCTGGCAGGCGATCAAAAACGATATTCGACAAAAAGAGCGTCGTCTTCTTGGTCGACGGCAGACGTTTCCGCCTATCTGAATCTGAAAAAACGGCTGACCTTGAGGGCGGCTATCTACAATATCGGCAACTACCGCTACGTTACTTGGGAATCCTTGCGCCAGACTGCGGAAAGCACGGCAAACCGGCACGGCGGCGACAGCAACTATGGAAGGTATGCCGCACCGGGCAGGAACTTCAGTCTCGCGCTCGAAATGAAGTTTTAA
>BBa_K1590012 (PotD)
ATGAAAAAATGGTCACGCCACCTGCTCGCGGCGGGTGCTCTGGCACTGGGCATGAGCGCCGCTCACGCCGATGACAACAACACGCTGTATTTCTACAACTGGACCGAGTACGTGCCGCCAGGACTGCTTGAACAGTTCACCAAAGAAACCGGTATTAAGGTTATCTATTCGACTTACGAGTCGAACGAAACCATGTACGCCAAGCTGAAAACATACAAAGACGGTGCCTATGATCTGGTGGTTCCTTCAACCTATTACGTTGATAAAATGCGTAAAGAAGGGATGATCCAGAAGATCGACAAGTCGAAGTTAACAAACTTCAGCAATCTCGATCCAGACATGCTCAACAAGCCTTTTGACCCGAATAACGACTACTCCATTCCGTATATCTGGGGTGCGACGGCGATTGGTGTTAACGGTGATGCGGTGGATCCGAAATCTGTCACCAGCTGGGCCGATCTGTGGAAGCCAGAGTACAAAGGCAGCCTGCTGTTGACCGACGATGCCCGTGAAGTGTTCCAGATGGCGCTGCGTAAGCTGGGCTACTCCGGTAACACCACCGATCCGAAAGAGATTGAAGCTGCATATAACGAGCTGAAAAAACTGATGCCAAACGTGGCAGCCTTTAACTCCGATAACCCGGCGAACCCGTACATGGAAGGCGAAGTTAACCTCGGCATGATCTGGAACGGTTCTGCTTTCGTTGCACGCCAGGCGGGTACGCCAATTGACGTGGTGTGGCCGAAAGAAGGCGGCATTTTCTGGATGGACAGCCTGGCGATCCCGGCAAATGCCAAAAACAAAGAAGGCGCGCTGAAATTGATCAACTTCCTGCTGCGCCCGGATGTGGCAAAACAGGTTGCTGAAACTATCGGTTATCCAACGCCAAACCTTGCGGCGCGTAAGCTGTTAAGTCCAGAAGTGGCGAACGATAAAACACTCTACCCGGATGCTGAAACCATTAAAAATGGCGAATGGCAGAATGACGTTGGCGCAGCCAGCAGCATTTATGAAGAGTATTATCAGAAGCTGAAAGCAGGACGTTAA
>BBa_K1590013 (Spermine Binding Protein)
ATGCTGCTGCTGCTGACCCTGGCGTTCCTGGCGTCTCCGACCTGCCGTGCGCAGAACGTTCTGGGTAACGCGGCGGGTAAATACTTCTACGTTCAGGGTGAAGACCAGGGTCAGCTGAAAGGTATGCGTATCTTCCTGTCTGTTTTCAAATTCATCAAAGGTTTCCAGCTCCAGTTCGGTTCTAACTGGACCGACGTTTACGGCACCCGTTCTGACAACTTCATCGACTTCCTGCTGGAAGACGGTGAACACGTTATCAAAGTTGAAGGTTCTGCGGTTATCTGCCTGACCTCTCTGACCTTCACCACCAACAAAGGTCGTGTTGCGACCTTCGGTGTTCGTCGTGGTCGTTACTTCTCTGACACCGGTGGTTCTGACAAACACCTGGTTACCGTTAACGGTATGCACGCGCCGGGTCTGTGCGTTCGTGGTATCGGTTTCAAATGGGGTAACATCAACGCGAACGGTAACGACCACTACAACAACAAAGAAGACAAAGCGGACAACAAAGACGCGGACAACAAAGACGCTGACAATAAAGACGATGGCGATGAGGATGATGATGGAAACGATGACGATGATCAGAAGGACGAATCTTAA
