Difference between revisions of "Team:Bielefeld-CeBiTec/Primers"
| Line 366: | Line 366: | ||
<tr id="T7-RBS-arsR-fw"> | <tr id="T7-RBS-arsR-fw"> | ||
<td>T7-RBS-arsR-fw</td> | <td>T7-RBS-arsR-fw</td> | ||
| − | <td> | + | <td>AGGGAGATACTAGAGAAAGAGGAGAAATACTAGATGTCATTTCTGTTACC </td> |
</tr> | </tr> | ||
<tr id="arsR-L-sfGFP-rv"> | <tr id="arsR-L-sfGFP-rv"> | ||
<td>arsR-L-sfGFP-rv</td> | <td>arsR-L-sfGFP-rv</td> | ||
| − | <td> | + | <td>CAGCTCTTCGCCTTTACGCATGCCGGTTAACTGCAAATGTTCTTAC</td> |
</tr> | </tr> | ||
<tr id="L-sfGFP-fw"> | <tr id="L-sfGFP-fw"> | ||
<td>L-sfGFP-fw</td> | <td>L-sfGFP-fw</td> | ||
| − | <td> | + | <td>ACCGGCATGCGTAAAGGCGAAGAGCTG</td> |
</tr> | </tr> | ||
<tr id="T7-RBS-arsR-rv"> | <tr id="T7-RBS-arsR-rv"> | ||
<td>T7-RBS-arsR-rv</td> | <td>T7-RBS-arsR-rv</td> | ||
| − | <td> | + | <td>CTAGTATTTCTCCTCTTTCTCTAGTATC</td> |
</tr> | </tr> | ||
<tr id="lacI-sfGFP-fw"> | <tr id="lacI-sfGFP-fw"> | ||
Revision as of 20:46, 21 August 2015
Primers
...
| Name | Sequence (5´ → 3´) | |
|---|---|---|
| Standard primers | ||
| SB-prep-2Ea | ATGAATTCCAGAAATCATCCTTAGCG | |
| SB-prep-3P-1 | GCCGCTGCAGTCCGGCAAAAAA | |
| VF | CCACCTGACGTCTAAGAA | |
| VR | GTATTACCGCCTTTGAGT | |
| Arsenic biosensor | ||
| Ars_1_rev | tatgacttaacgaatgtgtaagtgcagaggCGGATAAAACACATCTGAAAATTC | |
| Ars_2_fwd | cctctgcacttacacattcgttaagtcataGGTACTAGTAGCGGCC | |
| Ars_3_fwd | cattcgttaagtcatatatgtttttgacttGGTACTAGTAGCGGCC | |
| Ars_3_rev | tcaaaaacatatatgacttaacgaatgtgtCGGATAAAACACATCTGAAAATTC | |
| arsRhis_arsR_fwd | tatagggaaagaggagaaatactagagATGTCATTTCTGTTACCCATC | |
| arsRhis_arsR2_r | ctagtattagtggtgatggtgatgatgACTGCAAATGTTCTTACTG | |
| arsRhis_Trp_fwd | CATCATCACCATCACCAC | |
| arsRhis_Trp_rev | CTCTAGTATTTCTCCTCTTTCC | |
| UTR_arsO_rev | ccttcttttttttttttaaagttaaacaaaattatTAAGTCAAAAACATATATGACTTAACG | |
| UTR_noT7_rev | ccttcttttttttttttaaagttaaacaaaattattCTCTAGAAGCGGCCGCGA | |
| CFPS | ||
| Mut_gap_UTR_fwd | AGAATAATCTATGCGTAAAGGCGAAGAG | |
| pBS1C3_RraA_pre | ACAAAGCTCGGAAGTATCGTATTTCATCTCTAGAAGCGGCCGCGAATTCC | |
| pBS1C3_RraA_suf | TCAGAAGATCCGCTGGATATTGAATGATACTAGTAGCGGCCGCTGCAG | |
| pBS1C3_RraB_pre | TTCTTCCAGTTGTTCCGGGTTTGCCATCTCTAGAAGCGGCCGCGAATTCC | |
| pBS1C3_RraB_suf | GAAGACGATGACGGAGTTCGCCACTAATACTAGTAGCGGCCGCTGCAG | |
| pZ9_pT7_rne_fwd | gtccgcaacctgttgagtaaCTGCAGGAAGGGGATCCG | |
| pZ9_pT7_rne_rev | tagtatttctcctctttctctagtatctcCCTATAGTGAGTCGTATTAC | |
| rne_qPCR_fwd1 | GTGCTCGGCATAAGCCGAAG | |
| rne_qPCR_fwd2 | GCATAAGCCGAAGATATCG | |
| rne_qPCR_fwd3 | CCGAAGATATCGGTAGAGTT | |
| rne_qPCR_fwd4 | CTGCGCTACGGTAGCGAAAC | |
| rne_qPCR_rev2 | TTTGCGCCTCGTTATCATCC | |
| rne_to_pZ9_fwd | GATACTAGAGAAAGAGGAGAAATACTAGATGAAAAGAATGTTAATCAACGCAACTC | |
| rne_to_pZ9_rev | CCTGCAGTTACTCAACAGGTTGCGGACGCGCAG | |
| Rra_purif_fwd | TCAGAAGATCCGCTGGATATTGAATGATACTAGAGCCAGGCATCAAA | |
| RraA_fwd | ATGAAATACGATACTTCCGAGCTTTG | |
| RraA_purif_rev | ACAAAGCTCGGAAGTATCGTATTTCATGGCGCCCTGAAAATACAG | |
| RraA_rev | TCATTCAATATCCAGCGGATCTTC | |
| RraB_fwd | ATGGCAAACCCGGAACAACTG | |
| RraB_purif_fwd | GAAGACGATGACGGAGTTCGCCACTAATACTAGAGCCAGGCATCAAA | |
| RraB_purif_rev | TTCTTCCAGTTGTTCCGGGTTTGCCATGGCGCCCTGAAAATACAG | |
| RraB_rev | TTAGTGGCGAACTCCGTCATC | |
| SYFP_only_fwd | ATGGTTAGCAAGGGCGAAGA | |
| SYFP_only_rev | TTATTATTTATACAGCTCATCCATACC | |
| UTR_fwd | GTTTAACTTTAAAAAAAAAAAAGAAGGAGATATACATATGCGTAAAGGCGAAGAGCTGTTCAC | |
| UTR_rev | CCTTCTTTTTTTTTTTTAAAGTTAAACAAAATTATTTCTCCCTATAGTGAGTCGTATTACTCTAGAAG | |
| UTR_RFP_fwd | GTTTAACTTTAAAAAAAAAAAAGAAGGAGAATAATCTATGGCTTCCTCCGAAGACGTTATC | |
| UTR_SYFP_fwd | GTTTAACTTTAAAAAAAAAAAAGAAGGAGAATAATCTATGGTTAGCAAGGGCGAAGAAC | |
| Date rape drugs | ||
| BlcR_fwd | ATGGGCCAAAGGGGCCAAGT | |
| BlcR_rev | CTAGTCTTTCTGCGATCGGTACCC | |
| pSB1C3_BlcR_fwd | cttgggtaccgatcgcagaaagactagTACTAGTAGCGGCCGCTGCAG | |
| psB_BlcRfwd_new" | CTTGGGTACCGATCGCAGAAAGACTAATACTAGTAGCGGCCGCTGCAG | |
| pSB1C3_BlcR_rev | ttgacacacttggcccctttggcccatctagtaTTTCTCCTCTTTCTCTAGTAGCTAGCACAATAC | |
| BlcC_rev | TTACGCGTAAAATTCGGGAGCCTTC | |
| BlcC_rev_new | TTAGTCTTTCTGCGATCGGTACCC | |
| bclR-rv" | GTCTTTCTGCGATCGGTACCC | |
| blcR-CBD-fw | AAGCTTGGGTACCGATCGCAGAAAGACCCCGGCGCCAATCCGCCCGGT | |
| pro_Blc_fwd | ATGCACCTTCCTTGACACACT | |
| pro_Blc_rev | CATCGACCTCTCCCGCATTAG | |
| K516030proB_rev | gggccaagtgtgtcaaggaaggtgcatCTCTAGAAGCGGCCGCGAATTCC | |
| K516030proB_fwd | tgaactctaatgcgggagaggtcgATGGCTTCCTCCGAAGACGTTAT | |
| BlcB_mut_fwd | CCTTTCGAGTTCCGCACCGTTCCCT | |
| BlcB_mut_rev" | AGGGAACGGTGCGGAACTCGAAAGG | |
| BlcR-L-sfGFP-rv | TCGCCTTTACGCATGCCGGTGTCTTTCTGCGATCGGTACCC | |
| BlcR-T7-RBS-fw | TAGAGAAAGAGGAGAAATACTAGATGGGCCAAAG | |
| K60_BlcPshort_r | GAGTGAACTATGGCTCTAGTAGCTAGCACAATACC | |
| K60_BlcPshort_f" | GCTAGCTACTAGAGCCATAGTTCACTCTAATGATTC | |
| bclR-rv | GTCTTTCTGCGATCGGTACCC | |
| pSB1C3blcOnewfw | AAGAAGGCTCCCGAATTTTACGCGTAATACTAGTAGCGGCCGCTGCAG | |
| gBlocks | ||
| GABA_B_pSB1C3_f | ataagaaaataccgacagggggatattcacTACTAGTAGCGGCCGCTGC | |
| GABA_B_pSB1C3_r | cataaaaaaatcccagtcacaggtgattgaCTCTAGAAGCGGCCGCGA | |
| GABA_R_pSB1C3_f | tagttttcaaccctttcagcggaggagccatcgTACTAGTAGCGGCCGCTGC | |
| GABA_R_pSB1C3_r | ctgtggattaacacaccacctactttttaaCTCTAGAAGCGGCCGCGA | |
| Trp_sy_pSB1C3_f | aaagaagttcatcatcaccatcaccactaaTACTAGTAGCGGCCGCTGC | |
| Trp_sy_pSB1C3_r | tctcctctttccctatagtgagtcgtattaCTCTAGAAGCGGCCGCGA | |
| PRIA | ||
| T7-RBS-arsR-fw | AGGGAGATACTAGAGAAAGAGGAGAAATACTAGATGTCATTTCTGTTACC | |
| arsR-L-sfGFP-rv | CAGCTCTTCGCCTTTACGCATGCCGGTTAACTGCAAATGTTCTTAC | |
| L-sfGFP-fw | ACCGGCATGCGTAAAGGCGAAGAGCTG | |
| T7-RBS-arsR-rv | CTAGTATTTCTCCTCTTTCTCTAGTATC | |
| lacI-sfGFP-fw | ... | |
| T7-RBS-lacI-rv | ... | |
| C3-lacI-His-fw | ... | |
| UberHis-arsR-fw | ... | |
| arsR-UberHis_rv | ... | |
| BlcR-T7-RBS-fw | ... | |
| BlcR-L-sfGFP-rv | ... | |
| arsO-fw | ACATTCGTTAAGTCATATATGTTTTTGACTTA | |
| arsO-rv | TGTAAGCAATTCAGTATATACAAAAACTGAAT | |
| SuffixR-fw | CTGCAGCGGCCGCTACTAGTA | |
| lacO-klein | ATTTGCTTTGTGAGCGGATAACAAT | |
| lacO-klein | TGTGGAATTGTGAGCGGATAACAATTACG | |
| lacI-CBD-fw | ... | |
| lacI-CBD-Vektor-rv | ... | |
| EndHis-Vektor-lacI-fw | ... | |
| lacI-rv | ... | |
| arsR-dCBD-fw | ... | |
| CBD-eHisTerm-rv | ... | |
| arsR-rv | ... | |
| bclR-CBD-fw | ... | |
| CBD-eGFPTerm-rv | ... | |
| eGFP-dTerm-fw | ... | |
| bclR-rv | ... | |
| Split primers | ||
| Cm_fwd | CGGCATCAGCACCTTGTC | |
| Cm_rev | TATACGCAAGGCGACAAG | |
The gene-specific part of the primer is written in uppercase letters, while lowercase letters denote an overhang.